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. 1996 Jan;110(1):89–96. doi: 10.1104/pp.110.1.89

Coordinated Regulation of the Genes Participating in Starch Biosynthesis by the Rice Floury-2 Locus.

T Kawasaki 1, K Mizuno 1, H Shimada 1, H Satoh 1, N Kishimoto 1, S Okumura 1, N Ichikawa 1, T Baba 1
PMCID: PMC157697  PMID: 12226172

Abstract

The recessive floury-2 (flo-2) locus of rice (Oryza sativa L.), which is located on chromosome 4, causes a strong reduction in expression of the gene encoding an isoform of branching enzyme RBE1 in immature seeds 10 d after flowering. Mapping of the RBE1 gene demonstrated the localization on rice chromosome 6, suggesting that the wild-type Floury-2 (Flo-2) gene regulates RBE1 gene expression in trans. However, reduced expression of the genes encoding some other starch-synthesizing enzymes, including another isoform of branching enzyme RBE3 and granule-bound starch synthase, was also found in the flo-2 seeds. In spite of the low level of RBE1 gene expression in the immature seeds of the flo-2 mutants, the RBE1 gene was equally expressed in the leaves of the wild type and flo-2 mutants. Thus, these results imply that the Flo-2 gene may co-regulate expression of some of the genes participating in starch synthesis possibly in a developing seed-specific manner.

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Selected References

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