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. 1985 May;60(2):316–322.

Detection and partial characterization of human B cell colony stimulating activity in synovial fluids of patients with rheumatoid arthritis.

A C Fay, A Trudgett, J D McCrea, F Kirk, J M Thompson, E S Mitchell, M J Boyd, S D Roberts, T A McNeill
PMCID: PMC1577028  PMID: 3874024

Abstract

The joint fluids of 37 patients with rheumatoid arthritis, eight patients with traumatic injuries to their joints, two patients with Reiter's syndrome and three patients with psoriatic arthritis were tested for the presence of B cell colony stimulating activity (B cell CSA). B cell CSA was found in all of the joint fluids from the patients with rheumatoid arthritis but in none of the joint fluids from patients with traumatic injuries to their joints or in the joint fluids from the patients with Reiter's syndrome. A trace of B cell CSA was found in the joint fluid of one of the three patients with psoriatic arthritis. There was a positive correlation (r = 0.796) between the amount of rheumatoid factor present in the joint fluids and the titre of B cell CSA. This correlation was highly significant (P less than 0.001). The B cell CSA was localized to component(s) with molecular weight ranges 115-129 kD and 64-72 kD and an isoelectric point of 6.8. Its activity was sensitive to reduction with 2-mercaptoethanol and to the oxidising action of potassium periodate.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Al-Balaghi S., Ström H., Möller E. B cell differentiation factor in synovial fluid of patients with rheumatoid arthritis. Immunol Rev. 1984 Apr;78:7–23. doi: 10.1111/j.1600-065x.1984.tb00474.x. [DOI] [PubMed] [Google Scholar]
  2. Butler J. L., Ambrus J. L., Jr, Fauci A. S. Characterization of monoclonal B cell growth factor (BCGF) produced by a human T-T hybridoma. J Immunol. 1984 Jul;133(1):251–255. [PubMed] [Google Scholar]
  3. Fauci A. S., Lane H. C., Volkman D. J. Activation and regulation of human immune responses: implications in normal and disease states. Ann Intern Med. 1983 Jul;99(1):61–75. doi: 10.7326/0003-4819-99-1-61. [DOI] [PubMed] [Google Scholar]
  4. Fay A. C., Trudgett A., Thompson J. M., McNeill T. A. Human B cell colony growth from pre-B cells in vitro. Clin Exp Immunol. 1985 Mar;59(3):629–636. [PMC free article] [PubMed] [Google Scholar]
  5. Gordon J., Ley S. C., Melamed M. D., English L. S., Hughes-Jones N. C. Immortalized B lymphocytes produce B-cell growth factor. Nature. 1984 Jul 12;310(5973):145–147. doi: 10.1038/310145a0. [DOI] [PubMed] [Google Scholar]
  6. HEDBERG H. STUDIES ON THE DEPRESSED HEMOLYTIC COMPLEMENT ACTIVITY OF SYNOVIAL FLUID IN ADULT RHEUMATOID ARTHRITIS. Acta Rheumatol Scand. 1963;9:165–193. [PubMed] [Google Scholar]
  7. Moll J. M., Wright V. Psoriatic arthritis. Semin Arthritis Rheum. 1973;3(1):55–78. doi: 10.1016/0049-0172(73)90035-8. [DOI] [PubMed] [Google Scholar]
  8. Munthe E., Natvig J. B. Immunglobulin classes, subclasses and complexes of IgG rheumatoid factor in rheumatoid plasma cells. Clin Exp Immunol. 1972 Sep;12(1):55–70. [PMC free article] [PubMed] [Google Scholar]
  9. Möller E., Ström H., Al-Balaghi S. Role of polyclonal activation in specific immune responses. Relevance for findings of antibody activity in various diseases. Scand J Immunol. 1980;12(3):177–182. doi: 10.1111/j.1365-3083.1980.tb00057.x. [DOI] [PubMed] [Google Scholar]
  10. Theofilopoulos A. N., Dixon F. J. Autoimmune diseases: immunopathology and etiopathogenesis. Am J Pathol. 1982 Sep;108(3):319–365. [PMC free article] [PubMed] [Google Scholar]
  11. Trudgett A., Fay A. C., Thompson J. M., McNeill T. A. Biochemical and physiochemical characteristics of a pre-B cell stimulating factor produced by the plasmacytoma cell line LICR LON HMY2. Clin Exp Immunol. 1985 Mar;59(3):637–643. [PMC free article] [PubMed] [Google Scholar]
  12. Tsoi M. S., Aprile J., Dobbs S., Goehle S., Storb R. Enrichment (and depletion) of human suppressor cells with monoclonal antibodies and immunoglobulin-coated plates. J Immunol Methods. 1982 Sep 30;53(3):293–305. doi: 10.1016/0022-1759(82)90176-4. [DOI] [PubMed] [Google Scholar]
  13. Yoshizaki K., Nakagawa T., Fukunaga K., Kaieda T., Maruyama S., Kishimoto S., Yamamura Y., Kishimoto T. Characterization of human B cell growth factor (BCGF) from cloned T cells or mitogen-stimulated T cells. J Immunol. 1983 Mar;130(3):1241–1246. [PubMed] [Google Scholar]
  14. Zvaifler N. J., Schur P. Reactions of aggregated mercaptoethanol treated gamma globulin with rheumatoid factor--precipitin and complement fixation studies. Arthritis Rheum. 1968 Aug;11(4):523–536. doi: 10.1002/art.1780110402. [DOI] [PubMed] [Google Scholar]
  15. Zvaifler N. J. The immunopathology of joint inflammation in rheumatoid arthritis. Adv Immunol. 1973;16(0):265–336. doi: 10.1016/s0065-2776(08)60299-0. [DOI] [PubMed] [Google Scholar]

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