Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1996 Jan;110(1):155–162. doi: 10.1104/pp.110.1.155

Phytochrome A regulates red-light induction of phototropic enhancement in Arabidopsis.

B M Parks 1, P H Quail 1, R P Hangarter 1
PMCID: PMC157704  PMID: 8587979

Abstract

Phytochrome A (phyA) and phytochrome B photoreceptors have distinct roles in the regulation of plant growth and development. Studies using specific photomorphogenic mutants and transgenic plants overexpressing phytochrome have supported an evolving picture in which phyA and phytochrome B are responsive to continuous far-red and red light, respectively. Photomorphogenic mutants of Arabidopsis thaliana that had been selected for their inability to respond to continuous irradiance conditions were tested for their ability to carry out red-light-induced enhancement of phototropism, which is an inductive phytochrome response. We conclude that phyA is the primary photoreceptor regulating this response and provide evidence suggesting that a common regulatory domain in the phyA polypeptide functions for both high-irradiance and inductive phytochrome responses.

Full Text

The Full Text of this article is available as a PDF (1.3 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boylan M., Douglas N., Quail P. H. Dominant negative suppression of arabidopsis photoresponses by mutant phytochrome A sequences identifies spatially discrete regulatory domains in the photoreceptor. Plant Cell. 1994 Mar;6(3):449–460. doi: 10.1105/tpc.6.3.449. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  3. Clack T., Mathews S., Sharrock R. A. The phytochrome apoprotein family in Arabidopsis is encoded by five genes: the sequences and expression of PHYD and PHYE. Plant Mol Biol. 1994 Jun;25(3):413–427. doi: 10.1007/BF00043870. [DOI] [PubMed] [Google Scholar]
  4. Dehesh K., Franci C., Parks B. M., Seeley K. A., Short T. W., Tepperman J. M., Quail P. H. Arabidopsis HY8 locus encodes phytochrome A. Plant Cell. 1993 Sep;5(9):1081–1088. doi: 10.1105/tpc.5.9.1081. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Feldman L. J., Briggs W. R. Light-regulated gravitropism in seedling roots of maize. Plant Physiol. 1987;83:241–243. doi: 10.1104/pp.83.2.241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kiss J. Z., Hertel R., Sack F. D. Amyloplasts are necessary for full gravitropic sensitivity in roots of Arabidopsis thaliana. Planta. 1989;177:198–206. [PubMed] [Google Scholar]
  7. Liscum E., Briggs W. R. Mutations in the NPH1 locus of Arabidopsis disrupt the perception of phototropic stimuli. Plant Cell. 1995 Apr;7(4):473–485. doi: 10.1105/tpc.7.4.473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Liscum E., Hangarter R. P. Genetic Evidence That the Red-Absorbing Form of Phytochrome B Modulates Gravitropism in Arabidopsis thaliana. Plant Physiol. 1993 Sep;103(1):15–19. doi: 10.1104/pp.103.1.15. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McArthur J. A. Effect of red light on geotropism in pea epicotyls. Plant Physiol. 1979 Jan;63(1):218–220. doi: 10.1104/pp.63.1.218. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nagatani A., Reed J. W., Chory J. Isolation and Initial Characterization of Arabidopsis Mutants That Are Deficient in Phytochrome A. Plant Physiol. 1993 May;102(1):269–277. doi: 10.1104/pp.102.1.269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nick P., Schafer E. Interaction of gravi- and phototropic stimulation in the response of maize (Zea mays L.) coleoptiles. Planta. 1988 Feb;173(2):213–220. doi: 10.1007/BF00403013. [DOI] [PubMed] [Google Scholar]
  12. Parks B. M., Quail P. H. hy8, a new class of arabidopsis long hypocotyl mutants deficient in functional phytochrome A. Plant Cell. 1993 Jan;5(1):39–48. doi: 10.1105/tpc.5.1.39. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Quail P. H., Boylan M. T., Parks B. M., Short T. W., Xu Y., Wagner D. Phytochromes: photosensory perception and signal transduction. Science. 1995 May 5;268(5211):675–680. doi: 10.1126/science.7732376. [DOI] [PubMed] [Google Scholar]
  14. Quail P. H., Briggs W. R., Chory J., Hangarter R. P., Harberd N. P., Kendrick R. E., Koornneef M., Parks B., Sharrock R. A., Schafer E. Spotlight on Phytochrome Nomenclature. Plant Cell. 1994 Apr;6(4):468–471. doi: 10.1105/tpc.6.4.468. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Reed J. W., Nagatani A., Elich T. D., Fagan M., Chory J. Phytochrome A and Phytochrome B Have Overlapping but Distinct Functions in Arabidopsis Development. Plant Physiol. 1994 Apr;104(4):1139–1149. doi: 10.1104/pp.104.4.1139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Reed J. W., Nagpal P., Poole D. S., Furuya M., Chory J. Mutations in the gene for the red/far-red light receptor phytochrome B alter cell elongation and physiological responses throughout Arabidopsis development. Plant Cell. 1993 Feb;5(2):147–157. doi: 10.1105/tpc.5.2.147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Shinomura T., Nagatani A., Chory J., Furuya M. The Induction of Seed Germination in Arabidopsis thaliana Is Regulated Principally by Phytochrome B and Secondarily by Phytochrome A. Plant Physiol. 1994 Feb;104(2):363–371. doi: 10.1104/pp.104.2.363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Somers D. E., Sharrock R. A., Tepperman J. M., Quail P. H. The hy3 Long Hypocotyl Mutant of Arabidopsis Is Deficient in Phytochrome B. Plant Cell. 1991 Dec;3(12):1263–1274. doi: 10.1105/tpc.3.12.1263. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wagner D., Quail P. H. Mutational analysis of phytochrome B identifies a small COOH-terminal-domain region critical for regulatory activity. Proc Natl Acad Sci U S A. 1995 Sep 12;92(19):8596–8600. doi: 10.1073/pnas.92.19.8596. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Whitelam G. C., Johnson E., Peng J., Carol P., Anderson M. L., Cowl J. S., Harberd N. P. Phytochrome A null mutants of Arabidopsis display a wild-type phenotype in white light. Plant Cell. 1993 Jul;5(7):757–768. doi: 10.1105/tpc.5.7.757. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Xu Y., Parks B. M., Short T. W., Quail P. H. Missense mutations define a restricted segment in the C-terminal domain of phytochrome A critical to its regulatory activity. Plant Cell. 1995 Sep;7(9):1433–1443. doi: 10.1105/tpc.7.9.1433. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES