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. 1984 Dec;58(3):566–572.

IgE induces secretion of prostaglandin E2 by human monocytes.

J H Passwell, B Geiger, M Levanon, J Davidsohn, B E Cohen, B Ramot
PMCID: PMC1577113  PMID: 6334580

Abstract

IgE was isolated from a patient with the hyper IgE, recurrent infection syndrome by immunoadsorption on sepharose bound goat anti-human IgE. Addition of this IgE to a monolayer culture of human monocytes resulted in a dose-dependent increase in PGE2 secretion. The addition of F(ab')2 fraction of goat anti-human IgE in the presence of sub-stimulating doses of IgE markedly increased PGE2 secretion; whereas addition of F(ab')2 fragment of irrelevant goat IgG had no effect. Similar activation of monocytes which could be enhanced by anti IgE was observed in the presence of the patient's serum. No such effect was seen in the presence of normal human serum. These results indicate that IgE may activate human monocytes and induce PGE secretion.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baum J., Mowat A. G., Kirk J. A. A simplified method for the measurement of chemotaxis of polymorphonuclear leukocytes from human blood. J Lab Clin Med. 1971 Mar;77(3):501–509. [PubMed] [Google Scholar]
  2. Berger M., Kirkpatrick C. H., Goldsmith P. K., Gallin J. I. IgE antibodies to Staphylococcus aureus and Candida albicans in patients with the syndrome of hyperimmunoglobulin E and recurrent infections. J Immunol. 1980 Dec;125(6):2437–2443. [PubMed] [Google Scholar]
  3. Buckley R. H., Wray B. B., Belmaker E. Z. Extreme hyperimmunoglobulinemia E and undue susceptibility to infection. Pediatrics. 1972 Jan;49(1):59–70. [PubMed] [Google Scholar]
  4. Capron A., Dessaint J. P., Capron M., Joseph M., Torpier G. Effector mechanisms of immunity to schistosomes and their regulation. Immunol Rev. 1982;61:41–66. doi: 10.1111/j.1600-065x.1982.tb00373.x. [DOI] [PubMed] [Google Scholar]
  5. Fischer A., Durandy A., Griscelli C. Role of prostaglandin E2 in the induction of nonspecific T lymphocyte suppressor activity. J Immunol. 1981 Apr;126(4):1452–1455. [PubMed] [Google Scholar]
  6. Fiser P. M., Buckley R. H. Human IgE biosynthesis in vitro: studies with atopic and normal blood mononuclear cells and subpopulations. J Immunol. 1979 Oct;123(4):1788–1794. [PubMed] [Google Scholar]
  7. Flower R. J. Drugs which inhibit prostaglandin biosynthesis. Pharmacol Rev. 1974 Mar;26(1):33–67. [PubMed] [Google Scholar]
  8. Geha R. S., Reinherz E., Leung D., McKee K. T., Jr, Schlossman S., Rosen F. S. Deficiency of suppressor T cells in the hyperimmunoglobulin E syndrome. J Clin Invest. 1981 Sep;68(3):783–791. doi: 10.1172/JCI110315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Goodwin J. S., Messner R. P., Peake G. T. Prostaglandin suppression of mitogen-stimulated lymphocytes in vitro. Changes with mitogen dose and preincubation. J Clin Invest. 1978 Oct;62(4):753–760. doi: 10.1172/JCI109186. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hill H. R., Quie P. G. Raised serum-IgE levels and defective neutrophil chemotaxis in three children with eczema and recurrent bacterial infections. Lancet. 1974 Feb 9;1(7850):183–187. doi: 10.1016/s0140-6736(74)92493-3. [DOI] [PubMed] [Google Scholar]
  11. Ishizaka K., Ishizaka T. Mechanisms of reaginic hypersensitivity and IgE antibody response. Immunol Rev. 1978;41:109–148. doi: 10.1111/j.1600-065x.1978.tb01462.x. [DOI] [PubMed] [Google Scholar]
  12. Joseph M., Tonnel A. B., Capron A., Voisin C. Enzyme release and superoxide anion production by human alveolar macrophages stimulated with immunoglobulin E. Clin Exp Immunol. 1980 May;40(2):416–422. [PMC free article] [PubMed] [Google Scholar]
  13. Levine L., Gjtierrez Cernosek R. M., Van Vunakis H. Specificities of prostaglandins B 1 , F 1 , and F 2 antigen-antibody reactions. J Biol Chem. 1971 Nov 25;246(22):6782–6785. [PubMed] [Google Scholar]
  14. Melewicz F. M., Kline L. E., Cohen A. B., Spiegelberg H. L. Characterization of Fc receptors for IgE on human alveolar macrophages. Clin Exp Immunol. 1982 Aug;49(2):364–370. [PMC free article] [PubMed] [Google Scholar]
  15. Melewicz F. M., Plummer J. M., Spiegelberg H. L. Comparison of the Fc receptors for IgE on human lymphocytes and monocytes. J Immunol. 1982 Aug;129(2):563–569. [PubMed] [Google Scholar]
  16. NISONOFF A., WISSLER F. C., LIPMAN L. N., WOERNLEY D. L. Separation of univalent fragments from the bivalent rabbit antibody molecule by reduction of disulfide bonds. Arch Biochem Biophys. 1960 Aug;89:230–244. doi: 10.1016/0003-9861(60)90049-7. [DOI] [PubMed] [Google Scholar]
  17. Passwell J. H., Dayer J. M., Merler E. Increased prostaglandin production by human monocytes after membrane receptor activation. J Immunol. 1979 Jul;123(1):115–120. [PubMed] [Google Scholar]
  18. Passwell J., Rosen F. S., Merler E. The effect of Fc fragments of IgG on human mononuclear cell responses. Cell Immunol. 1980 Jul 1;52(2):395–403. doi: 10.1016/0008-8749(80)90360-3. [DOI] [PubMed] [Google Scholar]
  19. Quie P. G., White J. G., Holmes B., Good R. A. In vitro bactericidal capacity of human polymorphonuclear leukocytes: diminished activity in chronic granulomatous disease of childhood. J Clin Invest. 1967 Apr;46(4):668–679. doi: 10.1172/JCI105568. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Reinherz E. L., Schlossman S. F. The differentiation and function of human T lymphocytes. Cell. 1980 Apr;19(4):821–827. doi: 10.1016/0092-8674(80)90072-0. [DOI] [PubMed] [Google Scholar]
  21. Schopfer K., Baerlocher K., Price P., Krech U., Quie P. G., Douglas S. D. Staphylococcal IgE antibodies, hyperimmunoglobulinemia E and Staphylococcus aureus infections. N Engl J Med. 1979 Apr 12;300(15):835–838. doi: 10.1056/NEJM197904123001506. [DOI] [PubMed] [Google Scholar]
  22. Shou L., Schwartz S. A., Good R. A. Suppressor cell activity after concanavalin A treatment of lymphocytes from normal donors. J Exp Med. 1976 May 1;143(5):1100–1110. doi: 10.1084/jem.143.5.1100. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Stobo J. D. Immunosuppression in man: suppression by macrophages can be mediated by interactions with regulatory T cells. J Immunol. 1977 Sep;119(3):918–924. [PubMed] [Google Scholar]
  24. Stobo J. D., Kennedy M. S., Goldyne M. E. Prostaglandin E modulation of the mitogenic response of human T cells. Differential response of T-cell subpopulations. J Clin Invest. 1979 Nov;64(5):1188–1203. doi: 10.1172/JCI109572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Vane J. R. Prostaglandins as mediators of inflammation. Adv Prostaglandin Thromboxane Res. 1976;2:791–801. [PubMed] [Google Scholar]

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