Abstract
A late embryogenesis abundant (LEA) protein gene, HVA1, from barley (Hordeum vulgare L.) was introduced into rice suspension cells using the Biolistic-mediated transformation method, and a large number of independent transgenic rice (Oryza sativa L.) plants were generated. Expression of the barley HVA1 gene regulated by the rice actin 1 gene promoter led to high-level, constitutive accumulation of the HVA1 protein in both leaves and roots of transgenic rice plants. Second-generation transgenic rice plants showed significantly increased tolerance to water deficit and salinity. Transgenic rice plants maintained higher growth rates than nontransformed control plants under stress conditions. The increased tolerance was also reflected by delayed development of damage symptoms caused by stress and by improved recovery upon the removal of stress conditions. We also found that the extent of increased stress tolerance correlated with the level of the HVA1 protein accumulated in the transgenic rice plants. Using a transgenic approach, this study provides direct evidence supporting the hypothesis that LEA proteins play an important role in the protection of plants under water-or salt-stress conditions. Thus, LEA genes hold considerable potential for use as molecular tools for genetic crop improvement toward stress tolerance.
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- Blackman S. A., Obendorf R. L., Leopold A. C. Maturation proteins and sugars in desiccation tolerance of developing soybean seeds. Plant Physiol. 1992 Sep;100(1):225–230. doi: 10.1104/pp.100.1.225. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Blackman S. A., Wettlaufer S. H., Obendorf R. L., Leopold A. C. Maturation proteins associated with desiccation tolerance in soybean. Plant Physiol. 1991 Jul;96(3):868–874. doi: 10.1104/pp.96.3.868. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bradford K. J., Chandler P. M. Expression of "Dehydrin-Like" Proteins in Embryos and Seedlings of Zizania palustris and Oryza sativa during Dehydration. Plant Physiol. 1992 Jun;99(2):488–494. doi: 10.1104/pp.99.2.488. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
- Close T. J., Fenton R. D., Moonan F. A view of plant dehydrins using antibodies specific to the carboxy terminal peptide. Plant Mol Biol. 1993 Oct;23(2):279–286. doi: 10.1007/BF00029004. [DOI] [PubMed] [Google Scholar]
- Curry J., Walker-Simmons M. K. Unusual sequence of group 3 LEA (II) mRNA inducible by dehydration stress in wheat. Plant Mol Biol. 1993 Mar;21(5):907–912. doi: 10.1007/BF00027121. [DOI] [PubMed] [Google Scholar]
- Dure L., 3rd A repeating 11-mer amino acid motif and plant desiccation. Plant J. 1993 Mar;3(3):363–369. doi: 10.1046/j.1365-313x.1993.t01-19-00999.x. [DOI] [PubMed] [Google Scholar]
- Epstein E., Norlyn J. D., Rush D. W., Kingsbury R. W., Kelley D. B., Cunningham G. A., Wrona A. F. Saline culture of crops: a genetic approach. Science. 1980 Oct 24;210(4468):399–404. doi: 10.1126/science.210.4468.399. [DOI] [PubMed] [Google Scholar]
- Holmström K. O., Welin B., Mandal A., Kristiansdottir I., Teeri T. H., Lamark T., Strøm A. R., Palva E. T. Production of the Escherichia coli betaine-aldehyde dehydrogenase, an enzyme required for the synthesis of the osmoprotectant glycine betaine, in transgenic plants. Plant J. 1994 Nov;6(5):749–758. doi: 10.1046/j.1365-313x.1994.6050749.x. [DOI] [PubMed] [Google Scholar]
- Hong B., Barg R., Ho T. H. Developmental and organ-specific expression of an ABA- and stress-induced protein in barley. Plant Mol Biol. 1992 Feb;18(4):663–674. doi: 10.1007/BF00020009. [DOI] [PubMed] [Google Scholar]
- Moons A., Bauw G., Prinsen E., Van Montagu M., Van der Straeten D. Molecular and physiological responses to abscisic acid and salts in roots of salt-sensitive and salt-tolerant Indica rice varieties. Plant Physiol. 1995 Jan;107(1):177–186. doi: 10.1104/pp.107.1.177. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mundy J., Chua N. H. Abscisic acid and water-stress induce the expression of a novel rice gene. EMBO J. 1988 Aug;7(8):2279–2286. doi: 10.1002/j.1460-2075.1988.tb03070.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rathinasabapathi B., McCue K. F., Gage D. A., Hanson A. D. Metabolic engineering of glycine betaine synthesis: plant betaine aldehyde dehydrogenases lacking typical transit peptides are targeted to tobacco chloroplasts where they confer betaine aldehyde resistance. Planta. 1994;193(2):155–162. doi: 10.1007/BF00192524. [DOI] [PubMed] [Google Scholar]
- Ried J. L., Walker-Simmons M. K. Group 3 Late Embryogenesis Abundant Proteins in Desiccation-Tolerant Seedlings of Wheat (Triticum aestivum L.). Plant Physiol. 1993 May;102(1):125–131. doi: 10.1104/pp.102.1.125. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Saneoka H., Nagasaka C., Hahn D. T., Yang W. J., Premachandra G. S., Joly R. J., Rhodes D. Salt Tolerance of Glycinebetaine-Deficient and -Containing Maize Lines. Plant Physiol. 1995 Feb;107(2):631–638. doi: 10.1104/pp.107.2.631. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Seffens W. S., Almoguera C., Wilde H. D., Vonder Haar R. A., Thomas T. L. Molecular analysis of a phylogenetically conserved carrot gene: developmental and environmental regulation. Dev Genet. 1990;11(1):65–76. doi: 10.1002/dvg.1020110108. [DOI] [PubMed] [Google Scholar]
- Skriver K., Mundy J. Gene expression in response to abscisic acid and osmotic stress. Plant Cell. 1990 Jun;2(6):503–512. doi: 10.1105/tpc.2.6.503. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Still D. W., Kovach D. A., Bradford K. J. Development of Desiccation Tolerance during Embryogenesis in Rice (Oryza sativa) and Wild Rice (Zizania palustris) (Dehydrin Expression, Abscisic Acid Content, and Sucrose Accumulation). Plant Physiol. 1994 Feb;104(2):431–438. doi: 10.1104/pp.104.2.431. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Straub P. F., Shen Q., Ho T. D. Structure and promoter analysis of an ABA- and stress-regulated barley gene, HVA1. Plant Mol Biol. 1994 Oct;26(2):617–630. doi: 10.1007/BF00013748. [DOI] [PubMed] [Google Scholar]
- Tarczynski M. C., Jensen R. G., Bohnert H. J. Stress protection of transgenic tobacco by production of the osmolyte mannitol. Science. 1993 Jan 22;259(5094):508–510. doi: 10.1126/science.259.5094.508. [DOI] [PubMed] [Google Scholar]