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. 1985 Jun;60(3):546–552.

Monoclonal antibodies to a 28,000 mol. wt protein antigen of Mycobacterium leprae.

D B Young, M J Fohn, S R Khanolkar, T M Buchanan
PMCID: PMC1577202  PMID: 2410167

Abstract

Monoclonal antibodies (MoAb) have been used to analyse a protein antigen from Mycobacterium leprae with a subunit mol. wt of 28,000 daltons. Three different patterns of species specificity were observed with two antibodies being specific for M. leprae, two partially specific, and one broadly cross-reactive amongst all mycobacteria. Competitive binding and sandwich assays demonstrated that the specific and partially specific antibodies recognized closely related regions of the molecule while the cross-reactive antibody recognized a spatially separate epitope on the same polypeptide chain. Identification of specific and cross-reactive epitopes on a single antigenic molecule may be of considerable importance for understanding the functioning of the cell-mediated immune system during leprosy infection and the use of MoAb for such analyses is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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