Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1985 Sep;61(3):556–561.

In vitro correction of the interleukin-2 and interferon-gamma defect in multiple sclerosis.

G Vervliet, L Schandené
PMCID: PMC1577270  PMID: 3935348

Abstract

The effect of phytohaemagglutinin (PHA) and/or phorbol myristic acetate (PMA) on human interferon-gamma (HuIFN-gamma) and interleukin 2 (IL-2) production was measured in peripheral blood leucocytes (PBL) from multiple sclerosis (MS) patients. Seven out of 12 MS patients studied had PBLs which were unable to produce any detectable HuIFN-gamma after stimulation with PHA. The PBL cultures of the same seven patients were also defective for IL-2 production after PHA stimulation. Addition of PMA before and during PHA stimulation resulted in restored IL-2 and HuIFN-gamma production in all otherwise non-responsive cultures. In MS cultures responsive to PHA alone, the addition of PMA resulted in a 10-fold increase of HuIFN-gamma and IL-2 production. Analysis of interleukin 1 (IL-1) production by peripheral blood monocytes (PBM) isolated from the same cultures, revealed that both spontaneous and PMA-induced IL-1 yield was the same for all cultures tested, regardless whether they produced IL-2 and HuIFN-gamma after PHA stimulation or not.

Full text

PDF
556

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Farrar J. J., Benjamin W. R., Hilfiker M. L., Howard M., Farrar W. L., Fuller-Farrar J. The biochemistry, biology, and role of interleukin 2 in the induction of cytotoxic T cell and antibody-forming B cell responses. Immunol Rev. 1982;63:129–166. doi: 10.1111/j.1600-065x.1982.tb00414.x. [DOI] [PubMed] [Google Scholar]
  2. Farrar J. J., Mizel S. B., Fuller-Farrar J., Farrar W. L., Hilfiker M. L. Macrophage-independent activation of helper T cells. I. Production of Interleukin 2. J Immunol. 1980 Aug;125(2):793–798. [PubMed] [Google Scholar]
  3. Farrar W. L., Johnson H. M., Farrar J. J. Regulation of the production of immune interferon and cytotoxic T lymphocytes by interleukin 2. J Immunol. 1981 Mar;126(3):1120–1125. [PubMed] [Google Scholar]
  4. Kung P., Goldstein G., Reinherz E. L., Schlossman S. F. Monoclonal antibodies defining distinctive human T cell surface antigens. Science. 1979 Oct 19;206(4416):347–349. doi: 10.1126/science.314668. [DOI] [PubMed] [Google Scholar]
  5. Kurtzke J. F. Neurologic impairment in multiple sclerosis and the disability status scale. Acta Neurol Scand. 1970;46(4):493–512. doi: 10.1111/j.1600-0404.1970.tb05808.x. [DOI] [PubMed] [Google Scholar]
  6. Kwong C. H., Mueller G. C. Effects of hexachlorocyclohexane isomers on concanavalin A 'capping' in bovine lymphocytes. Biochim Biophys Acta. 1979 Sep 3;586(3):501–511. doi: 10.1016/0304-4165(79)90040-0. [DOI] [PubMed] [Google Scholar]
  7. Linker-Israeli M., Bakke A. C., Kitridou R. C., Gendler S., Gillis S., Horwitz D. A. Defective production of interleukin 1 and interleukin 2 in patients with systemic lupus erythematosus (SLE). J Immunol. 1983 Jun;130(6):2651–2655. [PubMed] [Google Scholar]
  8. Maizel A. L., Mehta S. R., Ford R. J., Lachman L. B. Effect of interleukin 1 on human thymocytes and purified human T cells. J Exp Med. 1981 Feb 1;153(2):470–475. doi: 10.1084/jem.153.2.470. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mizel S. B., Rosenstreich D. L., Oppenheim J. J. Phorbol myristic acetate stimulates LAF production by the macrophage cell line, P388D. Cell Immunol. 1978 Sep 15;40(1):230–235. doi: 10.1016/0008-8749(78)90330-1. [DOI] [PubMed] [Google Scholar]
  10. Neighbour P. A., Miller A. E., Bloom B. R. Interferon responses of leukocytes in multiple sclerosis. Neurology. 1981 May;31(5):561–566. doi: 10.1212/wnl.31.5.561. [DOI] [PubMed] [Google Scholar]
  11. Palacios R., Martinez-Maza O., De Ley M. Production of human immune interferon (Hu IFN-gamma) studied at the single cell level. Origin, evidence for spontaneous secretion and effect of cyclosporin A. Eur J Immunol. 1983 Mar;13(3):221–225. doi: 10.1002/eji.1830130308. [DOI] [PubMed] [Google Scholar]
  12. Palacios R. Mechanism of T cell activation: role and functional relationship of HLA-DR antigens and interleukins. Immunol Rev. 1982;63:73–110. doi: 10.1111/j.1600-065x.1982.tb00412.x. [DOI] [PubMed] [Google Scholar]
  13. Salonen R., Ilonen J., Reunanen M., Nikoskelainen J., Salmi A. PPD-, PWM-, and PHA-induced interferon in stable multiple sclerosis: association with HLA-Dw2 antigen and clinical variables. Ann Neurol. 1982 Mar;11(3):279–284. doi: 10.1002/ana.410110308. [DOI] [PubMed] [Google Scholar]
  14. Santoro T. J., Luger T. A., Ravache E. S., Smolen J. S., Oppenheim J. J., Steinberg A. D. In vitro correction of the interleukin 2 defect of autoimmune mice. Eur J Immunol. 1983 Jul;13(7):601–604. doi: 10.1002/eji.1830130717. [DOI] [PubMed] [Google Scholar]
  15. Vervliet G., Carton H., Meulepas E., Billiau A. Interferon production by cultured peripheral leucocytes of MS patients. Clin Exp Immunol. 1984 Oct;58(1):116–126. [PMC free article] [PubMed] [Google Scholar]
  16. Vervliet G., Claeys H., Van Haver H., Carton H., Vermylen C., Meulepas E., Billiau A. Interferon production and natural killer (NK) activity in leukocyte cultures from multiple sclerosis patients. J Neurol Sci. 1983 Jul;60(1):137–150. doi: 10.1016/0022-510x(83)90133-8. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES