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. 1986 Jan;63(1):105–110.

Responsiveness to live M. tuberculosis, and common antigens, of sonicate-stimulated T cell lines from normal donors.

G A Rook, J Steele, S Barnass, J Mace, J L Stanford
PMCID: PMC1577333  PMID: 3082544

Abstract

Live mycobacteria are more efficient vaccines against mycobacterial disease than killed ones. A possible explanation is the existence of important protective antigens released by live bacilli, which are not present in any significant quantity in dead ones. Conversely, internal mycobacterial antigens may be irrelevant to protection if not released by live bacilli. We show here, using T cell lines derived by limiting dilution from the peripheral blood of normal donors stimulated with sonicated BCG, that a variable percentage of sonicate responsive T cells is unable to respond to live M. tuberculosis. The possibility that such lines have an immunopathological, rather than protective role, is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bahr G. M., Rook G. A., Stanford J. L. Inhibition of the proliferative response of peripheral blood lymphocytes to mycobacterial or fungal antigens by co-stimulation with antigens from various mycobacterial species. Immunology. 1981 Nov;44(3):593–598. [PMC free article] [PubMed] [Google Scholar]
  2. Brown J. A., Stone M. M., Sutherland I. B.C.G. vaccination of children against leprosy in Uganda: results at end of second follow-up. Br Med J. 1968 Jan 6;1(5583):24–27. doi: 10.1136/bmj.1.5583.24. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Comstock G. W., Palmer C. E. Long-term results of BCG vaccination in the southern United States. Am Rev Respir Dis. 1966 Feb;93(2):171–183. doi: 10.1164/arrd.1966.93.2.171. [DOI] [PubMed] [Google Scholar]
  4. Eckels D. D., Lamb J. R., Lake P., Woody J. N., Johnson A. H., Hartzman R. J. Antigen-specific human T-lymphocyte clones. Genetic restriction of influenza virus-specific responses to HLA-D region genes. Hum Immunol. 1982 Jul;4(4):313–324. doi: 10.1016/0198-8859(82)90004-0. [DOI] [PubMed] [Google Scholar]
  5. Edwards M. L., Goodrich J. M., Muller D., Pollack A., Ziegler J. E., Smith D. W. Infection with Mycobacterium avium-intracellulare and the protective effects of Bacille Calmette-Guérin. J Infect Dis. 1982 May;145(5):733–741. doi: 10.1093/infdis/145.2.733. [DOI] [PubMed] [Google Scholar]
  6. Lema E., Stanford J. Skin-test sensitisation by tubercle bacilli and by other mycobacteria in Ethiopian school-children. Tubercle. 1984 Dec;65(4):285–293. doi: 10.1016/0041-3879(84)90038-2. [DOI] [PubMed] [Google Scholar]
  7. Rabin H., Hopkins R. F., 3rd, Ruscetti F. W., Neubauer R. H., Brown R. L., Kawakami T. G. Spontaneous release of a factor with properties of T cell growth factor from a continuous line of primate tumor T cells. J Immunol. 1981 Nov;127(5):1852–1856. [PubMed] [Google Scholar]
  8. Rook G. A., Champion B. R., Steele J., Varey A. M., Stanford J. L. I-A restricted activation by T cell lines of anti-tuberculosis activity in murine macrophages. Clin Exp Immunol. 1985 Feb;59(2):414–420. [PMC free article] [PubMed] [Google Scholar]
  9. Rook G. A. The immunogenicity of killed mycobacteria. Lepr Rev. 1980 Dec;51(4):295–301. doi: 10.5935/0305-7518.19800030. [DOI] [PubMed] [Google Scholar]
  10. WEISS D. W. Vaccination against tuberculosis with nonliving vaccines. I. The problem and its historical background. Am Rev Respir Dis. 1959 Nov;80:676–688. doi: 10.1164/arrd.1959.80.5.676. [DOI] [PubMed] [Google Scholar]
  11. van Oers M. H., Pinkster J., Zeijlemaker W. P. Quantification of antigen-reactive cells among human T lymphocytes. Eur J Immunol. 1978 Jul;8(7):477–484. doi: 10.1002/eji.1830080706. [DOI] [PubMed] [Google Scholar]

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