Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1986 Jan;63(1):111–117.

Cell mediated immunity cross-reactions of mycobacteria: polymorphism of target bacterial antigens.

S Kulkarni, S Hattikudur, R S Kamat
PMCID: PMC1577342  PMID: 3082545

Abstract

Swiss white mice were immunized with different mycobacteria and delayed type hypersensitivity (DTH) responses were studied by the foot-pad swelling technique of Gray and Jennings (1955). Extensive cross-reactions in DTH, outside the limits of Runyon's groups were observed. As a general trend slow growing mycobacteria showed greater cross-reactivity with slow growers than with rapid growers and vice versa. The implied cross-protective significance of DTH cross-reactions was further confirmed by demonstration of the ability of DTH cross-reacting sonicates to generate activated macrophages in M. avium immunized mice. An antiserum was raised against the earlier reported DTH eliciting antigen of M. tuberculosis H37Rv (DTH-H37Rv). The sero-reactivity of anti-DTH-H37Rv against the sonicates of different mycobacteria was studied with the objective of investigating the molecular basis of DTH cross-reactivity. Immunoprecipitation reactions of different mycobacterial sonicates with anti-DTH-H37Rv showed that the antigen was shared by all the mycobacteria tested irrespective of their cross-reactivity in a DTH response. All of the slow growers showed reactions of total identity with DTH-H37Rv. However with rapid growers DTH-H37Rv showed only a partial identity. From these data it was concluded that an antigen participating in DTH response is shared by all mycobacteria and that it is polymorphous, having genus specific and group specific (as slow and rapid grower groups) determinants.

Full text

PDF
111

Images in this article

115Fig. 2
on p.115

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blanden R. V., Mackaness G. B., Collins F. M. Mechanisms of acquired resistance in mouse typhoid. J Exp Med. 1966 Oct 1;124(4):585–600. doi: 10.1084/jem.124.4.585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chaparas S. D., Maloney C. J. An analysis of cross reactions among mycobacteria by in vivo and in vitro assays of cellular hypersensitivity. Am Rev Respir Dis. 1978 May;117(5):897–902. doi: 10.1164/arrd.1978.117.5.897. [DOI] [PubMed] [Google Scholar]
  3. Collins F. M. Immunogenicity of various mycobacteria and the corresponding levels of cross-protection developed between species. Infect Immun. 1971 Dec;4(6):688–696. doi: 10.1128/iai.4.6.688-696.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Collins F. M. Vaccines and cell-mediated immunity. Bacteriol Rev. 1974 Dec;38(4):371–402. doi: 10.1128/br.38.4.371-402.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DOUB L., YOUMANS G. P. Studies in tuberculosis chemotherapy; a simple primary aromatic amines, in vitro and in vivo. Am Rev Tuberc. 1950 Mar;61(3):407–421. [PubMed] [Google Scholar]
  6. GRAY D. F., JENNINGS P. A. Allergy in experimental mouse tuberculosis. Am Rev Tuberc. 1955 Aug;72(2):171–195. doi: 10.1164/artpd.1955.72.2.171. [DOI] [PubMed] [Google Scholar]
  7. Hattikudur S., Kamat R. S. Characterisation of target antigen of cell-mediated immunity in Mycobacterium tuberculosis H37RV. J Med Microbiol. 1984 Aug;18(1):17–25. doi: 10.1099/00222615-18-1-17. [DOI] [PubMed] [Google Scholar]
  8. Lefford M. J., Logie P. S. Induction and suppression of cross-reactive antituberculosis immunity after Mycobacterium lepraemurium infection of mice. Infect Immun. 1981 Mar;31(3):1023–1033. doi: 10.1128/iai.31.3.1023-1033.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. MACKANESS G. B. THE IMMUNOLOGICAL BASIS OF ACQUIRED CELLULAR RESISTANCE. J Exp Med. 1964 Jul 1;120:105–120. doi: 10.1084/jem.120.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. MAGNUSSON M. Specificity of mycobacterial sensitins. I. Studies in guinea pigs with purified "tuberculin" prepared from mammalian and avian tubercle bacilli, Mycobacterium balnei, and other acid-fast bacilli. Am Rev Respir Dis. 1961 Jan;83:57–68. doi: 10.1164/arrd.1961.83.1.57. [DOI] [PubMed] [Google Scholar]
  11. Nair R., Kamat R. S. Effector cell-mediated immune response in mice immunised with Salmonella. J Med Microbiol. 1982 May;15(2):215–221. doi: 10.1099/00222615-15-2-215. [DOI] [PubMed] [Google Scholar]
  12. Palmer C. E., Long M. W. Effects of infection with atypical mycobacteria on BCG vaccination and tuberculosis. Am Rev Respir Dis. 1966 Oct;94(4):553–568. doi: 10.1164/arrd.1966.94.4.553. [DOI] [PubMed] [Google Scholar]
  13. Shepard C. C., van Landingham R., Walker L. L. Searches among mycobacterial cultures for antileprosy vaccines. Infect Immun. 1980 Sep;29(3):1034–1039. doi: 10.1128/iai.29.3.1034-1039.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Stanford J. L., Rook G. A., Convit J., Godal T., Kronvall G., Rees R. J., Walsh G. P. Preliminary taxonomic studies on the leprosy bacillus. Br J Exp Pathol. 1975 Dec;56(6):579–585. [PMC free article] [PubMed] [Google Scholar]
  15. Youmans G. P. Relation between delayed hypersensitivity and immunity in tuberculosis. Am Rev Respir Dis. 1975 Feb;111(2):109–118. doi: 10.1164/arrd.1975.111.2.109. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES