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. 1986 Jan;63(1):179–187.

Human lymphocyte, monocyte and polymorphonuclear leucocyte mediated antibody-dependent cellular cytotoxicity against varicella-zoster virus-infected targets.

T Ihara, M Ito, S E Starr
PMCID: PMC1577353  PMID: 3955882

Abstract

The ability of lymphocytes, monocytes and polymorphonuclear leucocytes (PMN) to mediate antibody-dependent cellular cytotoxicity (ADCC) against varicella-zoster virus (VZV) infected fibroblasts was tested in 51Cr release and single-cell assays. Lymphocytes had the greatest lytic activity, monocytes were intermediate in activity and PMN were the least active. Lymphocyte-mediated ADCC was complete by as early as 4 h, while maximal monocyte and PMN-mediated ADCC required 18 h. In single-cell assays, monocytes formed conjugates with both uninfected and VZV-infected targets, but did not cause lysis. PMN failed to bind or lyse either target. Few lymphocytes formed conjugates with uninfected targets, while a higher percentage bound to VZV-infected targets and caused lysis. In the presence of human antibodies to VZV conjugate formation and lysis of VZV-infected targets was significantly increased with each of the effector-cell populations. Lymphocytes had the highest lytic activity in single-cell assays as well as in 51Cr release assays, and were responsible for most of the ADCC detected in adult peripheral blood against VZV-infected targets.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ackerman S. K., Douglas S. D. Purification of human monocytes on microexudate-coated surfaces. J Immunol. 1978 Apr;120(4):1372–1374. [PubMed] [Google Scholar]
  2. Babbage J., Sigfusson A., Souhami R. L. Antibody-dependent cell-mediated cytotoxicity to Varicella zoster. Clin Exp Immunol. 1984 Oct;58(1):217–222. [PMC free article] [PubMed] [Google Scholar]
  3. Ferrante A., Thong Y. H. Optimal conditions for simultaneous purification of mononuclear and polymorphonuclear leucocytes from human blood by the Hypaque-Ficoll method. J Immunol Methods. 1980;36(2):109–117. doi: 10.1016/0022-1759(80)90036-8. [DOI] [PubMed] [Google Scholar]
  4. Freundlich B., Trinchieri G., Perussia B., Zurier R. B. The cytotoxic effector cells in preparations of adherent mononuclear cells from human peripheral blood. J Immunol. 1984 Mar;132(3):1255–1260. [PubMed] [Google Scholar]
  5. Hashimoto G., Wright P. F., Karzon D. T. Antibody-dependent cell-mediated cytotoxicity against influenza virus-infected cells. J Infect Dis. 1983 Nov;148(5):785–794. doi: 10.1093/infdis/148.5.785. [DOI] [PubMed] [Google Scholar]
  6. Hiserodt J. C., Britvan L. J., Targan S. R. Characterization of the cytolytic reaction mechanism of the human natural killer (NK) lymphocyte: resolution into binding, programming, and killer cell-independent steps. J Immunol. 1982 Oct;129(4):1782–1787. [PubMed] [Google Scholar]
  7. Ihara T., Starr S. E., Ito M., Douglas S. D., Arbeter A. M. Human polymorphonuclear leukocyte-mediated cytotoxicity against varicella-zoster virus-infected fibroblasts. J Virol. 1984 Jul;51(1):110–116. doi: 10.1128/jvi.51.1.110-116.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Itoh K., Tilden A. B., Kumagai K., Balch C. M. Leu-11+ lymphocytes with natural killer (NK) activity are precursors of recombinant interleukin 2 (rIL 2)-induced activated killer (AK) cells. J Immunol. 1985 Feb;134(2):802–807. [PubMed] [Google Scholar]
  9. Kamiya H., Starr S. E., Arbeter A. M., Plotkin S. A. Antibody-dependent cell-mediated cytotoxicity against varicella-zoster virus-infected targets. Infect Immun. 1982 Nov;38(2):554–557. doi: 10.1128/iai.38.2.554-557.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kohl S., Starr S. E., oleske J. M., Shore S. L., Ashman R. B., Nahmias A. J. Human monocyte-macrophage-mediated antibody-dependent cytotoxicity to herpes simplex virus-infected cells. J Immunol. 1977 Mar;118(3):729–735. [PubMed] [Google Scholar]
  11. Kumagai K., Itoh K., Hinuma S., Tada M. Pretreatment of plastic Petri dishes with fetal calf serum. A simple method for macrophage isolation. J Immunol Methods. 1979;29(1):17–25. doi: 10.1016/0022-1759(79)90121-2. [DOI] [PubMed] [Google Scholar]
  12. Neville M. E., Grimm E., Bonavida B. Frequency determination of K cells by a single cell cytotoxic assay. J Immunol Methods. 1980;36(3-4):255–268. doi: 10.1016/0022-1759(80)90131-3. [DOI] [PubMed] [Google Scholar]
  13. Oleske J. M., Ashman R. B., Kohl S., Shore S. L., Starr S. E., Wood P., Nahmias A. J. Human polymorphonuclear leucocytes as mediators of antibody-dependent cellular cytotoxicity to herpes simplex virus-infected cells. Clin Exp Immunol. 1977 Mar;27(3):446–453. [PMC free article] [PubMed] [Google Scholar]
  14. Vadas M. A., Nicola N., Lopez A. F., Metcalf D., Johnson G., Pereira A. Mononuclear cell-mediated enhancement of granulocyte function in man. J Immunol. 1984 Jul;133(1):202–207. [PubMed] [Google Scholar]
  15. Williams V., Gershon A., Brunell P. A. Serologic response to varicella-zoster membrane antigens measured by direct immunofluorescence. J Infect Dis. 1974 Dec;130(6):669–672. doi: 10.1093/infdis/130.6.669. [DOI] [PubMed] [Google Scholar]

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