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. 1986 Feb;63(2):354–358.

Abnormalities of in vitro immunoglobulin production in apparently healthy haemophiliacs: relationship with alterations of T cell subsets and with HTLV-III seropositivity.

R Biagiotti, M G Giudizi, F Almerigogna, M Mazzetti, A Alessi, G F Del Prete, D Rafanelli, M Fiorilli, M Morfini, S Romagnani
PMCID: PMC1577369  PMID: 3009063

Abstract

The pokeweed mitogen (PWM)-induced immunoglobulin (Ig) production by cultures of peripheral blood mononuclear cells (PBMC) was reduced in healthy haemophiliacs treated with commercial factor VIII (or IX) concentrate, whereas the spontaneous IgG synthesis in vitro was enhanced. PWM-induced Ig production was lower in those who had received greater amounts of concentrate, in those with inverted T4/T8 lymphocyte ratios and in those with antibody to HTLV-III. The spontaneous IgG production in vitro was higher in haemophiliacs who had received larger amounts of concentrate, in those with inverted T4/T8 ratio and in those with antibody anti-HTLV-III. However, some patients with normal T4/T8 ratio and some with HTLV-III antibody also had raised spontaneous IgG production.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aiuti F., Rossi P., Sirianni M. C., Carbonari M., Popovic M., Sarngadharan M. G., Contu L., Moroni M., Romagnani S., Gallo R. C. IgM and IgG antibodies to human T cell lymphotropic retrovirus (HTLV-III) in lymphadenopathy syndrome and subjects at risk for AIDS in Italy. Br Med J (Clin Res Ed) 1985 Jul 20;291(6489):165–166. doi: 10.1136/bmj.291.6489.165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ammann A. J., Schiffman G., Abrams D., Volberding P., Ziegler J., Conant M. B-cell immunodeficiency in acquired immune deficiency syndrome. JAMA. 1984 Mar 16;251(11):1447–1449. [PubMed] [Google Scholar]
  3. Barré-Sinoussi F., Chermann J. C., Rey F., Nugeyre M. T., Chamaret S., Gruest J., Dauguet C., Axler-Blin C., Vézinet-Brun F., Rouzioux C. Isolation of a T-lymphotropic retrovirus from a patient at risk for acquired immune deficiency syndrome (AIDS). Science. 1983 May 20;220(4599):868–871. doi: 10.1126/science.6189183. [DOI] [PubMed] [Google Scholar]
  4. Elliott J. L., Hoppes W. L., Platt M. S., Thomas J. G., Patel I. P., Gansar A. The acquired immunodeficiency syndrome and Mycobacterium avium-intracellulare bacteremia in a patient with hemophilia. Ann Intern Med. 1983 Mar;98(3):290–293. doi: 10.7326/0003-4819-98-3-290. [DOI] [PubMed] [Google Scholar]
  5. Lane H. C., Masur H., Edgar L. C., Whalen G., Rook A. H., Fauci A. S. Abnormalities of B-cell activation and immunoregulation in patients with the acquired immunodeficiency syndrome. N Engl J Med. 1983 Aug 25;309(8):453–458. doi: 10.1056/NEJM198308253090803. [DOI] [PubMed] [Google Scholar]
  6. Mannucci P. M., Gringeri A., Ammassari M., Mari D. Abnormalities of lymphocyte subsets are correlated with concentrate consumption in asymptomatic Italian hemophiliacs treated with concentrates made from American plasma. Am J Hematol. 1984 Aug;17(2):167–176. doi: 10.1002/ajh.2830170208. [DOI] [PubMed] [Google Scholar]
  7. Melbye M., Froebel K. S., Madhok R., Biggar R. J., Sarin P. S., Stenbjerg S., Lowe G. D., Forbes C. D., Goedert J. J., Gallo R. C. HTLV-III seropositivity in European haemophiliacs exposed to Factor VIII concentrate imported from the USA. Lancet. 1984 Dec 22;2(8417-8418):1444–1446. doi: 10.1016/s0140-6736(84)91632-5. [DOI] [PubMed] [Google Scholar]
  8. Pahwa S. G., Quilop M. T., Lange M., Pahwa R. N., Grieco M. H. Defective B-lymphocyte function in homosexual men in relation to the acquired immunodeficiency syndrome. Ann Intern Med. 1984 Dec;101(6):757–763. doi: 10.7326/0003-4819-101-6-757. [DOI] [PubMed] [Google Scholar]
  9. Poon M. C., Landay A., Prasthofer E. F., Stagno S. Acquired immunodeficiency syndrome with Pneumocystis carinii pneumonia and Mycobacterium avium-intracellulare infection in a previously healthy patient with classic hemophilia. Clinical, immunologic, and virologic findings. Ann Intern Med. 1983 Mar;98(3):287–290. doi: 10.7326/0003-4819-98-3-287. [DOI] [PubMed] [Google Scholar]
  10. Popovic M., Sarngadharan M. G., Read E., Gallo R. C. Detection, isolation, and continuous production of cytopathic retroviruses (HTLV-III) from patients with AIDS and pre-AIDS. Science. 1984 May 4;224(4648):497–500. doi: 10.1126/science.6200935. [DOI] [PubMed] [Google Scholar]
  11. Romagnani S., Del Prete G. F., Maggi E., Bellesi G., Biti G., Rossi Ferrini P. L., Ricci M. Abnormalities of in vitro immunoglobulin synthesis by peripheral blood lymphocytes from untreated patients with Hodgkin's disease. J Clin Invest. 1983 May;71(5):1375–1382. doi: 10.1172/JCI110890. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Romagnani S., Del Prete G. F., Maggi E., Bosi A., Bernardi F., Ponticelli P., Di Lollo S., Ricci M. Displacement of T lymphocytes with the 'Helper/Inducer' phenotype from peripheral blood to lymphoid organs in untreated patients with Hodgkin's disease. Scand J Haematol. 1983 Oct;31(4):305–314. doi: 10.1111/j.1600-0609.1983.tb00658.x. [DOI] [PubMed] [Google Scholar]
  13. Salahuddin S. Z., Groopman J. E., Markham P. D., Sarngadharan M. G., Redfield R. R., McLane M. F., Essex M., Sliski A., Gallo R. C. HTLV-III in symptom-free seronegative persons. Lancet. 1984 Dec 22;2(8417-8418):1418–1420. doi: 10.1016/s0140-6736(84)91619-2. [DOI] [PubMed] [Google Scholar]
  14. Sarin P. S., Gallo R. C. Human T-lymphotropic retroviruses in adult T-cell leukemia-lymphoma and acquired immune deficiency syndrome. J Clin Immunol. 1984 Nov;4(6):415–423. doi: 10.1007/BF00916570. [DOI] [PubMed] [Google Scholar]

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