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. 1985 Oct;62(1):19–23.

Vaccination with a purified blood-stage malaria antigen in mice: correlation of protection with T cell mediated immunity.

J H Playfair, J B De Souza, R R Freeman, A A Holder
PMCID: PMC1577400  PMID: 2933196

Abstract

A purified 230,000 mol wt protein antigen from the lethal mouse malaria parasite Plasmodium yoelii YM which had previously been shown to be highly effective as a vaccine, was tested for its ability to stimulate specific helper T cells and T cells responsible for delayed hypersensitivity. Strong stimulation was found in both assays, but larger doses were required for delayed hypersensitivity, correlating well with the requirements for protection. It is suggested that T stimulation may be a requirement for effective protection by purified antigens in malaria.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Clark I. A., Virelizier J. L., Carswell E. A., Wood P. R. Possible importance of macrophage-derived mediators in acute malaria. Infect Immun. 1981 Jun;32(3):1058–1066. doi: 10.1128/iai.32.3.1058-1066.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cottrell B. J., Playfair J. H., De Souza B. J. Cell-mediated immunity in mice vaccinated against malaria. Clin Exp Immunol. 1978 Nov;34(2):147–158. [PMC free article] [PubMed] [Google Scholar]
  3. Dockrell H. M., Playfair J. H. Killing of blood-stage murine malaria parasites by hydrogen peroxide. Infect Immun. 1983 Jan;39(1):456–459. doi: 10.1128/iai.39.1.456-459.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Freeman R. R., Holder A. A. Characteristics of the protective response of BALB/c mice immunized with a purified Plasmodium yoelii schizont antigen. Clin Exp Immunol. 1983 Dec;54(3):609–616. [PMC free article] [PubMed] [Google Scholar]
  5. Freeman R. R., Trejdosiewicz A. J., Cross G. A. Protective monoclonal antibodies recognising stage-specific merozoite antigens of a rodent malaria parasite. Nature. 1980 Mar 27;284(5754):366–368. doi: 10.1038/284366a0. [DOI] [PubMed] [Google Scholar]
  6. Goodman J. W., Sercarz E. E. The complexity of structures involved in T-cell activation. Annu Rev Immunol. 1983;1:465–498. doi: 10.1146/annurev.iy.01.040183.002341. [DOI] [PubMed] [Google Scholar]
  7. Grun J. L., Weidanz W. P. Antibody-independent immunity to reinfection malaria in B-cell-deficient mice. Infect Immun. 1983 Sep;41(3):1197–1204. doi: 10.1128/iai.41.3.1197-1204.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Holder A. A., Freeman R. R. Characterization of a high molecular weight protective antigen of Plasmodium yoelii. Parasitology. 1984 Apr;88(Pt 2):211–219. [PubMed] [Google Scholar]
  9. Holder A. A., Freeman R. R. Immunization against blood-stage rodent malaria using purified parasite antigens. Nature. 1981 Nov 26;294(5839):361–364. doi: 10.1038/294361a0. [DOI] [PubMed] [Google Scholar]
  10. Holder A. A., Freeman R. R., Newbold C. I. Serological cross-reaction between high molecular weight proteins synthesized in blood schizonts of Plasmodium yoelii, Plasmodium chabaudi and Plasmodium falciparum. Mol Biochem Parasitol. 1983 Nov;9(3):191–196. doi: 10.1016/0166-6851(83)90096-8. [DOI] [PubMed] [Google Scholar]
  11. Kaufmann S. H., Hahn H. Biological functions of t cell lines with specificity for the intracellular bacterium Listeria monocytogenes in vitro and in vivo. J Exp Med. 1982 Jun 1;155(6):1754–1765. doi: 10.1084/jem.155.6.1754. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Majarian W. R., Daly T. M., Weidanz W. P., Long C. A. Passive immunization against murine malaria with an IgG3 monoclonal antibody. J Immunol. 1984 Jun;132(6):3131–3137. [PubMed] [Google Scholar]
  13. Ockenhouse C. F., Shear H. L. Oxidative killing of the intraerythrocytic malaria parasite Plasmodium yoelii by activated macrophages. J Immunol. 1984 Jan;132(1):424–431. [PubMed] [Google Scholar]
  14. Playfair J. H., De Souza J. B., Dockrell H. M., Agomo P. U., Taverne J. Cell-mediated immunity in the liver of mice vaccinated against malaria. Nature. 1979 Dec 13;282(5740):731–734. doi: 10.1038/282731a0. [DOI] [PubMed] [Google Scholar]
  15. Taverne J., Dockrell H. M., Playfair J. H. Endotoxin-induced serum factor kills malarial parasites in vitro. Infect Immun. 1981 Jul;33(1):83–89. doi: 10.1128/iai.33.1.83-89.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Taverne J., Dockrell H. M., Playfair J. H. Killing of the malarial parasite Plasmodium yoelii in vitro by cells of myeloid origin. Parasite Immunol. 1982 Mar;4(2):77–91. doi: 10.1111/j.1365-3024.1982.tb00421.x. [DOI] [PubMed] [Google Scholar]
  17. Weidanz W. P., Grun J. L. Antibody-independent mechanisms in the development of acquired immunity to malaria. Adv Exp Med Biol. 1983;162:409–423. doi: 10.1007/978-1-4684-4481-0_37. [DOI] [PubMed] [Google Scholar]

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