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. 1986 Mar;63(3):680–686.

Elution of antispectrin antibodies from red cells in homozygous beta-thalassaemia.

E Wiener, N C Hughes-Jones, W T Irish, S N Wickramasinghe
PMCID: PMC1577562  PMID: 3708907

Abstract

It is well-established that autoantibodies against red cell membrane antigens, such as spectrin are present in plasma of humans and animals. It is shown here that antispectrin IgG antibodies can be eluted from the red cells of patients with beta-thalassaemia intermedia. It is suggested that these antibodies could play a part in the increased rate of destruction of red cells in homozygous beta-thalassemia, either by reacting with spectrin which is abnormally exposed on the red cell surface or by a cross-reaction with a different membrane component. No rise was found in serum antispectrin levels in beta-thalassaemia; anti-red cell membrane IgG levels were slightly raised, but there was considerable overlap between the levels in patients and in normals. No autoantibody could be found in eluates from cells of normal subjects or patients with sickle cell anaemia.

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Selected References

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  1. Branch D. R., Hian A. L., Petz L. D. A new elution procedure using chloroform, a nonflammable organic solvent. Vox Sang. 1982 Jan;42(1):46–53. doi: 10.1111/j.1423-0410.1982.tb01068.x. [DOI] [PubMed] [Google Scholar]
  2. Cox K. O., Baddams H., Evans A. Studies of the antigenicity and immunogenicity of bromelain-pretreated red blood cells. Aust J Exp Biol Med Sci. 1977 Feb;55(1):27–37. doi: 10.1038/icb.1977.3. [DOI] [PubMed] [Google Scholar]
  3. Dighiero G., Lymberi P., Mazié J. C., Rouyre S., Butler-Browne G. S., Whalen R. G., Avrameas S. Murine hybridomas secreting natural monoclonal antibodies reacting with self antigens. J Immunol. 1983 Nov;131(5):2267–2272. [PubMed] [Google Scholar]
  4. Guilbert B., Dighiero G., Avrameas S. Naturally occurring antibodies against nine common antigens in human sera. I. Detection, isolation and characterization. J Immunol. 1982 Jun;128(6):2779–2787. [PubMed] [Google Scholar]
  5. HUGHES-JONES N. C., GARDNER B., TELFORD R. COMPARISON OF VARIOUS METHODS OF DISSOCIATION OF ANTI-D, USING 131I-LABELLED ANTIBODY. Vox Sang. 1963 Sep-Oct;8:531–536. doi: 10.1111/j.1423-0410.1963.tb04180.x. [DOI] [PubMed] [Google Scholar]
  6. Hellström I., Hellström K. E. Can "blocking" serum factors protect against autoimmunity? Nature. 1972 Dec 22;240(5382):471–473. doi: 10.1038/240471a0. [DOI] [PubMed] [Google Scholar]
  7. Kahane I., Shifter A., Rachmilewitz E. A. Cross-linking of red blood cell membrane proteins induced by oxidative stress in beta thalassemia. FEBS Lett. 1978 Jan 15;85(2):267–270. doi: 10.1016/0014-5793(78)80470-0. [DOI] [PubMed] [Google Scholar]
  8. Kay M. M. Localization of senescent cell antigen on band 3. Proc Natl Acad Sci U S A. 1984 Sep;81(18):5753–5757. doi: 10.1073/pnas.81.18.5753. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lutz H. U., Wipf G. Naturally occurring autoantibodies to skeletal proteins from human red blood cells. J Immunol. 1982 Apr;128(4):1695–1699. [PubMed] [Google Scholar]
  10. Marchesi V. T. The red cell membrane skeleton: recent progress. Blood. 1983 Jan;61(1):1–11. [PubMed] [Google Scholar]
  11. Martin W. J., Martin S. E. Thymus reactive IgM autoantibodies in normal mouse sera. Nature. 1975 Apr 24;254(5502):716–718. doi: 10.1038/254716a0. [DOI] [PubMed] [Google Scholar]
  12. Melamed M. D., Gordon J., Ley S. J., Edgar D., Hughes-Jones N. C. Senescence of a human lymphoblastoid clone producing anti-Rhesus(D). Eur J Immunol. 1985 Jul;15(7):742–746. doi: 10.1002/eji.1830150720. [DOI] [PubMed] [Google Scholar]
  13. Mollison P. L., Hughes-Jones N. C. Clearance of Rh-positive red cells by low concentrations of Rh antibody. Immunology. 1967 Jan;12(1):63–73. [PMC free article] [PubMed] [Google Scholar]
  14. Poncet P., Kocher H. P., Pages J., Jaton J. C., Bussard A. E. Monoclonal autoantibodies against mouse red blood cells: a family of structurally restricted molecules. Mol Immunol. 1985 May;22(5):541–551. doi: 10.1016/0161-5890(85)90177-4. [DOI] [PubMed] [Google Scholar]
  15. Rogentine G. N., Jr, Plocinik B. A. Carbohydrate inhibition studies of the naturally occurring human antibody to neuraminidase-treated human lymphocytes. J Immunol. 1974 Sep;113(3):848–858. [PubMed] [Google Scholar]
  16. Shotton D. M., Burke B. E., Branton D. The molecular structure of human erythrocyte spectrin. Biophysical and electron microscopic studies. J Mol Biol. 1979 Jun 25;131(2):303–329. doi: 10.1016/0022-2836(79)90078-0. [DOI] [PubMed] [Google Scholar]
  17. Steck T. L., Weinstein R. S., Straus J. H., Wallach D. F. Inside-out red cell membrane vesicles: preparation and purification. Science. 1970 Apr 10;168(3928):255–257. doi: 10.1126/science.168.3928.255. [DOI] [PubMed] [Google Scholar]
  18. Wahlgren M., Berzins K., Perlmann P., Björkman A. Characterization of the humoral immune response in Plasmodium falciparum malaria. I. Estimation of antibodies to P. falciparum or human erythrocytes by means of microELISA. Clin Exp Immunol. 1983 Oct;54(1):127–134. [PMC free article] [PubMed] [Google Scholar]
  19. Wiener E. A possible role for antibodies against spectrin in the interaction between erythroblasts and macrophages in vitro. Br J Exp Pathol. 1985 Feb;66(1):17–25. [PMC free article] [PubMed] [Google Scholar]
  20. Wiener E., Wickramasinghe S. N. Impaired protein synthesis in erythroblasts enhances their phagocytosis by macrophages. Br J Haematol. 1983 Jan;53(1):117–124. doi: 10.1111/j.1365-2141.1983.tb01993.x. [DOI] [PubMed] [Google Scholar]
  21. Wiener E., Wickramasinghe S. N. Interaction between erythroblasts and macrophages in vitro: effect of neuraminidase-treatment of erythroblasts and the role of serum factors. Br J Haematol. 1983 Oct;55(2):369–378. doi: 10.1111/j.1365-2141.1983.tb01258.x. [DOI] [PubMed] [Google Scholar]
  22. Winchester R. J., Fu S. M., Winfield J. B., Kunkel H. G. Immunofluorescent studies on antibodies directed to a buried membrane structure present in lymphocytes and erythrocytes. J Immunol. 1975 Jan;114(1 Pt 2):410–414. [PubMed] [Google Scholar]

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