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. 1996 Mar;110(3):979–986. doi: 10.1104/pp.110.3.979

Activation of Phospholipase A by Plant Defense Elicitors.

S Chandra 1, P F Heinstein 1, P S Low 1
PMCID: PMC157798  PMID: 12226235

Abstract

Participation of phospholipase A (PLase A) in plant signal transduction has been documented for auxin stimulation of growth but not for elicitation of any plant defense response. In this paper, we report two independent assays for monitoring PLase A induction in plant cells and have used these assays to evaluate whether transduction of defense-related signals might require PLase A activation. Oligogalacturonic acid, a potent elicitor of the soybean (Glycine max) H2O2 burst, was unable to stimulate endogenous PLase A, suggesting that PLase A activation is not an obligate intermediate in the oligogalacturonic acid-induced burst pathway. In contrast, harpin and an extract from the pathogenic fungus Verticillium dahliae both stimulated the oxidative burst and promoted a rapid increase in PLase A activity. To evaluate the possible role of this inducible PLase A activity in transducing the oxidative burst, we tested the effect of chlorpromazine-HCl, a PLase A inhibitor on elicitor-stimulated burst activity. Pretreatment with chloropromazine was found to inhibit the H2O2 burst triggered by V. dahliae extract at the same concentration at which it blocked PLase A activation. In contrast, neither the harpin- nor oligogalacturonic acid-induced burst was altered by addition of chlorpromazine. These data suggest that PLase A stimulation may be important in certain elicitor-induced oxidative bursts (e.g. V. dahliae) and that other elicitors such as oligogalacturonic acid and harpin must operate through independent signaling intermediates to activate the same defense response.

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Selected References

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  1. Apostol I., Heinstein P. F., Low P. S. Rapid Stimulation of an Oxidative Burst during Elicitation of Cultured Plant Cells : Role in Defense and Signal Transduction. Plant Physiol. 1989 May;90(1):109–116. doi: 10.1104/pp.90.1.109. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Axelrod J. Receptor-mediated activation of phospholipase A2 and arachidonic acid release in signal transduction. Biochem Soc Trans. 1990 Aug;18(4):503–507. doi: 10.1042/bst0180503. [DOI] [PubMed] [Google Scholar]
  3. Baker C. J., Orlandi E. W., Mock N. M. Harpin, An Elicitor of the Hypersensitive Response in Tobacco Caused by Erwinia amylovora, Elicits Active Oxygen Production in Suspension Cells. Plant Physiol. 1993 Aug;102(4):1341–1344. doi: 10.1104/pp.102.4.1341. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Blumenkrantz N., Asboe-Hansen G. New method for quantitative determination of uronic acids. Anal Biochem. 1973 Aug;54(2):484–489. doi: 10.1016/0003-2697(73)90377-1. [DOI] [PubMed] [Google Scholar]
  5. Bostock R. M., Kuc J. A., Laine R. A. Eicosapentaenoic and Arachidonic Acids from Phytophthora infestans Elicit Fungitoxic Sesquiterpenes in the Potato. Science. 1981 Apr 3;212(4490):67–69. doi: 10.1126/science.212.4490.67. [DOI] [PubMed] [Google Scholar]
  6. Choi D., Ward B. L., Bostock R. M. Differential induction and suppression of potato 3-hydroxy-3-methylglutaryl coenzyme A reductase genes in response to Phytophthora infestans and to its elicitor arachidonic acid. Plant Cell. 1992 Oct;4(10):1333–1344. doi: 10.1105/tpc.4.10.1333. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Creelman R. A., Tierney M. L., Mullet J. E. Jasmonic acid/methyl jasmonate accumulate in wounded soybean hypocotyls and modulate wound gene expression. Proc Natl Acad Sci U S A. 1992 Jun 1;89(11):4938–4941. doi: 10.1073/pnas.89.11.4938. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dennis E. A., Rhee S. G., Billah M. M., Hannun Y. A. Role of phospholipase in generating lipid second messengers in signal transduction. FASEB J. 1991 Apr;5(7):2068–2077. doi: 10.1096/fasebj.5.7.1901288. [DOI] [PubMed] [Google Scholar]
  9. Farmer E. E., Ryan C. A. Octadecanoid Precursors of Jasmonic Acid Activate the Synthesis of Wound-Inducible Proteinase Inhibitors. Plant Cell. 1992 Feb;4(2):129–134. doi: 10.1105/tpc.4.2.129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gil J., Higgins T., Rozengurt E. Mastoparan, a novel mitogen for Swiss 3T3 cells, stimulates pertussis toxin-sensitive arachidonic acid release without inositol phosphate accumulation. J Cell Biol. 1991 May;113(4):943–950. doi: 10.1083/jcb.113.4.943. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gross W., Yang W., Boss W. F. Release of carrot plasma membrane-associated phosphatidylinositol kinase by phospholipase A2 and activation by a 70 kDa protein. Biochim Biophys Acta. 1992 Feb 19;1134(1):73–80. doi: 10.1016/0167-4889(92)90029-b. [DOI] [PubMed] [Google Scholar]
  12. Gundlach H., Müller M. J., Kutchan T. M., Zenk M. H. Jasmonic acid is a signal transducer in elicitor-induced plant cell cultures. Proc Natl Acad Sci U S A. 1992 Mar 15;89(6):2389–2393. doi: 10.1073/pnas.89.6.2389. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hamberg M., Gardner H. W. Oxylipin pathway to jasmonates: biochemistry and biological significance. Biochim Biophys Acta. 1992 Nov 11;1165(1):1–18. doi: 10.1016/0005-2760(92)90069-8. [DOI] [PubMed] [Google Scholar]
  14. Higashijima T., Burnier J., Ross E. M. Regulation of Gi and Go by mastoparan, related amphiphilic peptides, and hydrophobic amines. Mechanism and structural determinants of activity. J Biol Chem. 1990 Aug 25;265(24):14176–14186. [PubMed] [Google Scholar]
  15. Kauss H., Jeblick W. Influence of Free Fatty Acids, Lysophosphatidylcholine, Platelet-Activating Factor, Acylcarnitine, and Echinocandin B on 1,3-beta-d-Glucan Synthase and Callose Synthesis. Plant Physiol. 1986 Jan;80(1):7–13. doi: 10.1104/pp.80.1.7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kuehl F. A., Jr, Egan R. W. Prostaglandins, arachidonic acid, and inflammation. Science. 1980 Nov 28;210(4473):978–984. doi: 10.1126/science.6254151. [DOI] [PubMed] [Google Scholar]
  17. Legendre L., Rueter S., Heinstein P. F., Low P. S. Characterization of the Oligogalacturonide-Induced Oxidative Burst in Cultured Soybean (Glycine max) Cells. Plant Physiol. 1993 May;102(1):233–240. doi: 10.1104/pp.102.1.233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Legendre L., Yueh Y. G., Crain R., Haddock N., Heinstein P. F., Low P. S. Phospholipase C activation during elicitation of the oxidative burst in cultured plant cells. J Biol Chem. 1993 Nov 25;268(33):24559–24563. [PubMed] [Google Scholar]
  19. Liscovitch M., Cantley L. C. Lipid second messengers. Cell. 1994 May 6;77(3):329–334. doi: 10.1016/0092-8674(94)90148-1. [DOI] [PubMed] [Google Scholar]
  20. Low P. S., Heinstein P. F. Elicitor stimulation of the defense response in cultured plant cells monitored by fluorescent dyes. Arch Biochem Biophys. 1986 Sep;249(2):472–479. doi: 10.1016/0003-9861(86)90024-x. [DOI] [PubMed] [Google Scholar]
  21. Martiny-Baron G., Scherer G. F. Phospholipid-stimulated protein kinase in plants. J Biol Chem. 1989 Oct 25;264(30):18052–18059. [PubMed] [Google Scholar]
  22. Matton D. P., Brisson N. Cloning, expression, and sequence conservation of pathogenesis-related gene transcripts of potato. Mol Plant Microbe Interact. 1989 Nov-Dec;2(6):325–331. doi: 10.1094/mpmi-2-325. [DOI] [PubMed] [Google Scholar]
  23. Morré D. J., Brightman A. O. NADH oxidase of plasma membranes. J Bioenerg Biomembr. 1991 Jun;23(3):469–489. doi: 10.1007/BF00771015. [DOI] [PubMed] [Google Scholar]
  24. Oishi K., Raynor R. L., Charp P. A., Kuo J. F. Regulation of protein kinase C by lysophospholipids. Potential role in signal transduction. J Biol Chem. 1988 May 15;263(14):6865–6871. [PubMed] [Google Scholar]
  25. Scherer G. F., André B. A rapid response to a plant hormone: auxin stimulates phospholipase A2 in vivo and in vitro. Biochem Biophys Res Commun. 1989 Aug 30;163(1):111–117. doi: 10.1016/0006-291x(89)92106-2. [DOI] [PubMed] [Google Scholar]
  26. Scherer G. F., Morré D. J. In vitro stimulation by 2,4-dichlorophenoxyacetic acid of an ATPase and inhibition of phosphatidate phosphatase of plant membranes. Biochem Biophys Res Commun. 1978 Sep 14;84(1):238–247. doi: 10.1016/0006-291x(78)90288-7. [DOI] [PubMed] [Google Scholar]
  27. Scheuer W. Phospholipase A2--regulation and inhibition. Klin Wochenschr. 1989 Feb 1;67(3):153–159. doi: 10.1007/BF01711343. [DOI] [PubMed] [Google Scholar]
  28. Sharp J. K., Valent B., Albersheim P. Purification and partial characterization of a beta-glucan fragment that elicits phytoalexin accumulation in soybean. J Biol Chem. 1984 Sep 25;259(18):11312–11320. [PubMed] [Google Scholar]
  29. Smith W. L., Marnett L. J., DeWitt D. L. Prostaglandin and thromboxane biosynthesis. Pharmacol Ther. 1991;49(3):153–179. doi: 10.1016/0163-7258(91)90054-p. [DOI] [PubMed] [Google Scholar]

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