Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1996 May;111(1):159–168. doi: 10.1104/pp.111.1.159

A thiol protease and an anionic peroxidase are induced by lowering cytokinins during callus growth in Petunia.

C Tournaire 1, S Kushnir 1, G Bauw 1, D Inzé 1, B Teyssendier de la Serve 1, J P Renaudin 1
PMCID: PMC157822  PMID: 8685263

Abstract

We previously identified a group of proteins that increase early in Petunia hybrida calli subcultured on a low-cytokinin medium, unlike the calli subcultured on a high-cytokinin medium. The calli on the low-cytokinin medium do not regenerate (J.-P. Renaudin, C. Tournaire, B, Teyssendier de la Serve [1991] Physiol Plant 82: 48-56). Two of these proteins, P21 and P17, have been identified by peptide sequencing and cloned. P21 is highly homologous to a group of thiol proteases, including barely aleurain, rice oryzain gamma, Arabidopsis SAG2, and mammalian cathepsin H. P17 is highly homologous to a group of anionic peroxidases from potato and tomato. A study of their expression in two P. hybrida lines, PC6 and St40 which differ in their ability to regenerate, showed that the genes for P21 and P17 are differentially expressed depending on the type and the age of the organ, with the highest expression in senescing leaves and in aged calli. The data are in favor of these genes being associated with an early step of senescence, which may be due, in part, to a reduction in total cytokinin. The two Petunia lines are, thus, functionally different concerning the action of cytokinin in two developmental phenomena: in vitro organogenesis and senescence.

Full Text

The Full Text of this article is available as a PDF (2.3 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abeles F. B., Dunn L. J., Morgens P., Callahan A., Dinterman R. E., Schmidt J. Induction of 33-kD and 60-kD Peroxidases during Ethylene-Induced Senescence of Cucumber Cotyledons. Plant Physiol. 1988 Jul;87(3):609–615. doi: 10.1104/pp.87.3.609. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Auer C. A., Cohen J. D. Identification of a benzyladenine disaccharide conjugate produced during shoot organogenesis in Petunia leaf explants. Plant Physiol. 1993 Jun;102(2):541–545. doi: 10.1104/pp.102.2.541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Auer C. A., Cohen J. D., Laloue M., Cooke T. J. Comparison of Benzyl Adenine Metabolism in Two Petunia hybrida Lines Differing in Shoot Organogenesis. Plant Physiol. 1992 Mar;98(3):1035–1041. doi: 10.1104/pp.98.3.1035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Baker E. N. Structure of actinidin, after refinement at 1.7 A resolution. J Mol Biol. 1980 Aug 25;141(4):441–484. doi: 10.1016/0022-2836(80)90255-7. [DOI] [PubMed] [Google Scholar]
  5. Benton W. D., Davis R. W. Screening lambdagt recombinant clones by hybridization to single plaques in situ. Science. 1977 Apr 8;196(4286):180–182. doi: 10.1126/science.322279. [DOI] [PubMed] [Google Scholar]
  6. Church G. M., Gilbert W. Genomic sequencing. Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991–1995. doi: 10.1073/pnas.81.7.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Corpet F. Multiple sequence alignment with hierarchical clustering. Nucleic Acids Res. 1988 Nov 25;16(22):10881–10890. doi: 10.1093/nar/16.22.10881. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Crowell D. N., Amasino R. M. Induction of Specific mRNAs in Cultured Soybean Cells during Cytokinin or Auxin Starvation. Plant Physiol. 1991 Mar;95(3):711–715. doi: 10.1104/pp.95.3.711. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Crowell D. N., John M. E., Russell D., Amasino R. M. Characterization of a stress-induced, developmentally regulated gene family from soybean. Plant Mol Biol. 1992 Feb;18(3):459–466. doi: 10.1007/BF00040662. [DOI] [PubMed] [Google Scholar]
  10. Davis R. W., Thomas M., Cameron J., St John T. P., Scherer S., Padgett R. A. Rapid DNA isolations for enzymatic and hybridization analysis. Methods Enzymol. 1980;65(1):404–411. doi: 10.1016/s0076-6879(80)65051-4. [DOI] [PubMed] [Google Scholar]
  11. Estruch J. J., Prinsen E., VAN Onckelen H., Schell J., Spena A. Viviparous leaves produced by somatic activation of an inactive cytokinin-synthesizing gene. Science. 1991 Nov 29;254(5036):1364–1367. doi: 10.1126/science.254.5036.1364. [DOI] [PubMed] [Google Scholar]
  12. Fuchs R., Machleidt W., Gassen H. G. Molecular cloning and sequencing of a cDNA coding for mature human kidney cathepsin H. Biol Chem Hoppe Seyler. 1988 Jun;369(6):469–475. doi: 10.1515/bchm3.1988.369.1.469. [DOI] [PubMed] [Google Scholar]
  13. Hensel L. L., Grbić V., Baumgarten D. A., Bleecker A. B. Developmental and age-related processes that influence the longevity and senescence of photosynthetic tissues in arabidopsis. Plant Cell. 1993 May;5(5):553–564. doi: 10.1105/tpc.5.5.553. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Holwerda B. C., Galvin N. J., Baranski T. J., Rogers J. C. In Vitro Processing of Aleurain, a Barley Vacuolar Thiol Protease. Plant Cell. 1990 Nov;2(11):1091–1106. doi: 10.1105/tpc.2.11.1091. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Holwerda B. C., Padgett H. S., Rogers J. C. Proaleurain vacuolar targeting is mediated by short contiguous peptide interactions. Plant Cell. 1992 Mar;4(3):307–318. doi: 10.1105/tpc.4.3.307. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Li Y., Hagen G., Guilfoyle T. J. Altered morphology in transgenic tobacco plants that overproduce cytokinins in specific tissues and organs. Dev Biol. 1992 Oct;153(2):386–395. doi: 10.1016/0012-1606(92)90123-x. [DOI] [PubMed] [Google Scholar]
  17. Lütcke H. A., Chow K. C., Mickel F. S., Moss K. A., Kern H. F., Scheele G. A. Selection of AUG initiation codons differs in plants and animals. EMBO J. 1987 Jan;6(1):43–48. doi: 10.1002/j.1460-2075.1987.tb04716.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Marchuk D., Drumm M., Saulino A., Collins F. S. Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products. Nucleic Acids Res. 1991 Mar 11;19(5):1154–1154. doi: 10.1093/nar/19.5.1154. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Ritonja A., Popović T., Kotnik M., Machleidt W., Turk V. Amino acid sequences of the human kidney cathepsins H and L. FEBS Lett. 1988 Feb 15;228(2):341–345. doi: 10.1016/0014-5793(88)80028-0. [DOI] [PubMed] [Google Scholar]
  20. Roberts E., Kolattukudy P. E. Molecular cloning, nucleotide sequence, and abscisic acid induction of a suberization-associated highly anionic peroxidase. Mol Gen Genet. 1989 Jun;217(2-3):223–232. doi: 10.1007/BF02464885. [DOI] [PubMed] [Google Scholar]
  21. Rogers J. C., Dean D., Heck G. R. Aleurain: a barley thiol protease closely related to mammalian cathepsin H. Proc Natl Acad Sci U S A. 1985 Oct;82(19):6512–6516. doi: 10.1073/pnas.82.19.6512. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. SKOOG F., MILLER C. O. Chemical regulation of growth and organ formation in plant tissues cultured in vitro. Symp Soc Exp Biol. 1957;11:118–130. [PubMed] [Google Scholar]
  23. Smart C. M., Scofield S. R., Bevan M. W., Dyer T. A. Delayed Leaf Senescence in Tobacco Plants Transformed with tmr, a Gene for Cytokinin Production in Agrobacterium. Plant Cell. 1991 Jul;3(7):647–656. doi: 10.1105/tpc.3.7.647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Teramoto H., Momotani E., Takeba G., Tsuji H. Isolation of a cDNA clone for a cytokinin-repressed gene in excised cucumber cotyledons. Planta. 1994;193(4):573–579. doi: 10.1007/BF02411564. [DOI] [PubMed] [Google Scholar]
  25. Teyssendier de la Serve B., Jouanneau J. P. Preferential incorporation of an exogenous cytokinin, N6-benzyladenine, into 18S and 25S ribosomal RNA of tobacco cells in suspension culture. Biochimie. 1979;61(8):913–922. doi: 10.1016/s0300-9084(79)80241-2. [DOI] [PubMed] [Google Scholar]
  26. Watanabe H., Abe K., Emori Y., Hosoyama H., Arai S. Molecular cloning and gibberellin-induced expression of multiple cysteine proteinases of rice seeds (oryzains). J Biol Chem. 1991 Sep 5;266(25):16897–16902. [PubMed] [Google Scholar]
  27. Watillon B., Kettmann R., Boxus P., Burny A. Characterization of Two Gene Transcripts Modulated by Cytokinins in Micropropagated Apple (Malus domestica [L.] Borkh) Plantlets. Plant Physiol. 1991 Jun;96(2):479–484. doi: 10.1104/pp.96.2.479. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES