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. 1996 May;111(1):301–312. doi: 10.1104/pp.111.1.301

Effects of COR6.6 and COR15am polypeptides encoded by COR (cold-regulated) genes of Arabidopsis thaliana on dehydration-induced phase transitions of phospholipid membranes.

M S Webb 1, S J Gilmour 1, M F Thomashow 1, P L Steponkus 1
PMCID: PMC157838  PMID: 8685270

Abstract

Cold acclimation of Arabidopsis thaliana includes the expression of cold-regulated (COR) genes and the accumulation of COR polypeptides. The hydration characteristics of two COR polypeptides, COR6.6 and COR15am, have been determined and their effects on the dehydration-induced liquid crystalline-to-gel and lamellar-to-hexagonal II phase transitions in phospholipid mixtures have been examined. After dehydration at osmotic pressures between 8 and 150 MPa, the water content of the COR polypeptides was less than that of bovine serum albumin, with COr15am the least hydrated: bovine serum albumin > COR6.6 > COR15am. Neither COR6.6 nor COR15am altered the dehydration-induced gel lamellar --> fluid lamellar phase transition temperature of either dipalmitoylphosphatidylcholine or dioleoylphosphatidylcholine (DOPC). In multilamellar vesicles of dioleoylphosphatidylethanolamine:DOPC (1:1, mol:mol) prepared by either freeze-thaw or reverse-phase evaporation methods, neither COR6.6, COR15am, nor bovine serum albumin altered the incidence of the dehydration-induced formation of the inverted hexagonal phase as a function of osmotic pressure. However, a specific ultrastructural alteration--the formation of a striated surface morphology in the lamellar domains--was observed in mixtures of dioleoylphosphatidylethanolamine:DOPC that were dehydrated in the presence of COR15am. Nevertheless, neither COR6.6 nor COR15am appears to participate in a specific protein-phospholipid interaction that alters the dehydration-induced phase behavior of phospholipid vesicles.

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Selected References

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  1. Gilmour S. J., Lin C., Thomashow M. F. Purification and properties of Arabidopsis thaliana COR (cold-regulated) gene polypeptides COR15am and COR6.6 expressed in Escherichia coli. Plant Physiol. 1996 May;111(1):293–299. doi: 10.1104/pp.111.1.293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Hincha D. K., DeVries A. L., Schmitt J. M. Cryotoxicity of antifreeze proteins and glycoproteins to spinach thylakoid membranes--comparison with cryotoxic sugar acids. Biochim Biophys Acta. 1993 Mar 14;1146(2):258–264. doi: 10.1016/0005-2736(93)90364-6. [DOI] [PubMed] [Google Scholar]
  3. Luna E. J., McConnell H. M. Multiple phase equilibria in binary mixtures of phospholipids. Biochim Biophys Acta. 1978 Jun 2;509(3):462–473. doi: 10.1016/0005-2736(78)90240-7. [DOI] [PubMed] [Google Scholar]
  4. Luna E. J., McConnell H. M. The intermediate monoclinic phase of phosphatidylcholines. Biochim Biophys Acta. 1977 May 2;466(3):381–392. doi: 10.1016/0005-2736(77)90331-5. [DOI] [PubMed] [Google Scholar]
  5. Mayer L. D., Hope M. J., Cullis P. R., Janoff A. S. Solute distributions and trapping efficiencies observed in freeze-thawed multilamellar vesicles. Biochim Biophys Acta. 1985 Jul 11;817(1):193–196. doi: 10.1016/0005-2736(85)90084-7. [DOI] [PubMed] [Google Scholar]
  6. Szoka F., Jr, Papahadjopoulos D. Procedure for preparation of liposomes with large internal aqueous space and high capture by reverse-phase evaporation. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4194–4198. doi: 10.1073/pnas.75.9.4194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Uemura M., Joseph R. A., Steponkus P. L. Cold Acclimation of Arabidopsis thaliana (Effect on Plasma Membrane Lipid Composition and Freeze-Induced Lesions). Plant Physiol. 1995 Sep;109(1):15–30. doi: 10.1104/pp.109.1.15. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Volger H. G., Heber U. Cryoprotective leaf proteins. Biochim Biophys Acta. 1975 Dec 15;412(2):335–349. doi: 10.1016/0005-2795(75)90048-3. [DOI] [PubMed] [Google Scholar]
  9. Webb M. S., Hui S. W., Steponkus P. L. Dehydration-induced lamellar-to-hexagonal-II phase transitions in DOPE/DOPC mixtures. Biochim Biophys Acta. 1993 Jan 18;1145(1):93–104. doi: 10.1016/0005-2736(93)90385-d. [DOI] [PubMed] [Google Scholar]
  10. Webb M. S., Steponkus P. L. Freeze-Induced Membrane Ultrastructural Alterations in Rye (Secale cereale) Leaves. Plant Physiol. 1993 Mar;101(3):955–963. doi: 10.1104/pp.101.3.955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Webb M. S., Uemura M., Steponkus P. L. A Comparison of Freezing Injury in Oat and Rye: Two Cereals at the Extremes of Freezing Tolerance. Plant Physiol. 1994 Feb;104(2):467–478. doi: 10.1104/pp.104.2.467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Zasadzinski J. A. Effect of stereoconfiguration on ripple phases (P beta') of dipalmitoylphosphatidylcholine. Biochim Biophys Acta. 1988 Dec 22;946(2):235–243. doi: 10.1016/0005-2736(88)90398-7. [DOI] [PubMed] [Google Scholar]

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