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. 1996 Jun;111(2):515–524. doi: 10.1104/pp.111.2.515

Heat-Shock Response in Heat-Tolerant and Nontolerant Variants of Agrostis palustris Huds.

S Y Park 1, R Shivaji 1, J V Krans 1, D S Luthe 1
PMCID: PMC157862  PMID: 12226306

Abstract

The heat-shock response in heat-tolerant variants (SB) and non-tolerant variants (NSB) of creeping bentgrass (Agrostis palustris Huds.) was investigated. Both variants were derived from callus initiated from a single seed of the cultivar Penncross. SB and NSB synthesized heat-shock proteins (HSPs) of 97, 83, 70, 40, 25, and 18 kD. There were no major differences between SB and NSB in the time or temperature required to induce the heat-shock response. When the HSPs synthesized by SB and NSB were analyzed by two-dimensional gel electrophoresis, it was apparent that SB synthesized two to three additional members of the HSP27 family, which were smaller (25 kD) and more basic than those synthesized by NSB. Analysis of F1 progeny of NSB x SB indicated that 7 of the 20 progeny did not synthesize the additional HSP25 polypeptides. These progeny were significantly less heat tolerant than progeny that did synthesize the additional HSP25 polypeptides. The X2 test of independence (X2 = 22.45, P < 0.001) indicated that heat tolerance and the presence of the additional HSP25 polypeptides are linked traits.

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Selected References

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  1. Baszczynski C. L., Walden D. B., Atkinson B. G. Regulation of gene expression in corn (Zea Mays L.) by heat shock. Can J Biochem. 1982 May;60(5):569–579. doi: 10.1139/o82-070. [DOI] [PubMed] [Google Scholar]
  2. Boyer J. S. Plant productivity and environment. Science. 1982 Oct 29;218(4571):443–448. doi: 10.1126/science.218.4571.443. [DOI] [PubMed] [Google Scholar]
  3. Fender S. E., O'connell M. A. Expression of the Heat Shock Response in a Tomato Interspecific Hybrid Is Not Intermediate between the Two Parental Responses. Plant Physiol. 1990 Jul;93(3):1140–1146. doi: 10.1104/pp.93.3.1140. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fish L. E., Jagendorf A. T. High rates of protein synthesis by isolated chloroplasts. Plant Physiol. 1982 Oct;70(4):1107–1114. doi: 10.1104/pp.70.4.1107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Helm K. W., Abernethy R. H. Heat Shock Proteins and Their mRNAs in Dry and Early Imbibing Embryos of Wheat. Plant Physiol. 1990 Aug;93(4):1626–1633. doi: 10.1104/pp.93.4.1626. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hernandez L. D., Vierling E. Expression of Low Molecular Weight Heat-Shock Proteins under Field Conditions. Plant Physiol. 1993 Apr;101(4):1209–1216. doi: 10.1104/pp.101.4.1209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hurkman W. J., Tanaka C. K. Solubilization of plant membrane proteins for analysis by two-dimensional gel electrophoresis. Plant Physiol. 1986 Jul;81(3):802–806. doi: 10.1104/pp.81.3.802. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hwang C. H., Zimmerman J. L. The Heat Shock Response of Carrot : Protein Variations between Cultured Cell Lines. Plant Physiol. 1989 Oct;91(2):552–558. doi: 10.1104/pp.91.2.552. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jakob U., Gaestel M., Engel K., Buchner J. Small heat shock proteins are molecular chaperones. J Biol Chem. 1993 Jan 25;268(3):1517–1520. [PubMed] [Google Scholar]
  10. Kimpel J. A., Key J. L. Presence of Heat Shock mRNAs in Field Crown Soybeans. Plant Physiol. 1985 Nov;79(3):672–678. doi: 10.1104/pp.79.3.672. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lee G. J., Pokala N., Vierling E. Structure and in vitro molecular chaperone activity of cytosolic small heat shock proteins from pea. J Biol Chem. 1995 May 5;270(18):10432–10438. doi: 10.1074/jbc.270.18.10432. [DOI] [PubMed] [Google Scholar]
  12. Lin C. Y., Roberts J. K., Key J. L. Acquisition of Thermotolerance in Soybean Seedlings : Synthesis and Accumulation of Heat Shock Proteins and their Cellular Localization. Plant Physiol. 1984 Jan;74(1):152–160. doi: 10.1104/pp.74.1.152. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  14. Osteryoung K. W., Vierling E. Dynamics of small heat shock protein distribution within the chloroplasts of higher plants. J Biol Chem. 1994 Nov 18;269(46):28676–28682. [PubMed] [Google Scholar]
  15. Smeekens S., Bauerle C., Hageman J., Keegstra K., Weisbeek P. The role of the transit peptide in the routing of precursors toward different chloroplast compartments. Cell. 1986 Aug 1;46(3):365–375. doi: 10.1016/0092-8674(86)90657-4. [DOI] [PubMed] [Google Scholar]
  16. Vierling E., Key J. L. Ribulose 1,5-Bisphosphate Carboxylase Synthesis during Heat Shock. Plant Physiol. 1985 May;78(1):155–162. doi: 10.1104/pp.78.1.155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Zhu J. K., Shi J., Bressan R. A., Hasegawa P. M. Expression of an Atriplex nummularia gene encoding a protein homologous to the bacterial molecular chaperone DnaJ. Plant Cell. 1993 Mar;5(3):341–349. doi: 10.1105/tpc.5.3.341. [DOI] [PMC free article] [PubMed] [Google Scholar]

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