Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1996 Jun;111(2):645–649. doi: 10.1104/pp.111.2.645

PIP1 Aquaporins Are Concentrated in Plasmalemmasomes of Arabidopsis thaliana Mesophyll.

D G Robinson 1, H Sieber 1, W Kammerloher 1, A R Schaffner 1
PMCID: PMC157878  PMID: 12226318

Abstract

The PIP1 subfamily of water channel proteins (aquaporins) constitute about 1% of the plasma membrane (PM) proteins from Arabidopsis thaliana leaves. Immunogold electron microscopy has confirmed their localization at the PM of mesophyll cells. Very high labeling density at PM invaginations known as plasmalemmasomes was observed. Therefore, we suggest that these subcellular structures are involved in water transport between the apoplast and the vacuole.

Full Text

The Full Text of this article is available as a PDF (1.3 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Daniels M. J., Mirkov T. E., Chrispeels M. J. The plasma membrane of Arabidopsis thaliana contains a mercury-insensitive aquaporin that is a homolog of the tonoplast water channel protein TIP. Plant Physiol. 1994 Dec;106(4):1325–1333. doi: 10.1104/pp.106.4.1325. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Herman E. M., Lamb C. J. Arabinogalactan-rich glycoproteins are localized on the cell surface and in intravacuolar multivesicular bodies. Plant Physiol. 1992 Jan;98(1):264–272. doi: 10.1104/pp.98.1.264. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hoh B., Hinz G., Jeong B. K., Robinson D. G. Protein storage vacuoles form de novo during pea cotyledon development. J Cell Sci. 1995 Jan;108(Pt 1):299–310. doi: 10.1242/jcs.108.1.299. [DOI] [PubMed] [Google Scholar]
  4. Kaldenhoff R., Kölling A., Meyers J., Karmann U., Ruppel G., Richter G. The blue light-responsive AthH2 gene of Arabidopsis thaliana is primarily expressed in expanding as well as in differentiating cells and encodes a putative channel protein of the plasmalemma. Plant J. 1995 Jan;7(1):87–95. doi: 10.1046/j.1365-313x.1995.07010087.x. [DOI] [PubMed] [Google Scholar]
  5. Knepper M. A. The aquaporin family of molecular water channels. Proc Natl Acad Sci U S A. 1994 Jul 5;91(14):6255–6258. doi: 10.1073/pnas.91.14.6255. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Maurel C., Reizer J., Schroeder J. I., Chrispeels M. J. The vacuolar membrane protein gamma-TIP creates water specific channels in Xenopus oocytes. EMBO J. 1993 Jun;12(6):2241–2247. doi: 10.1002/j.1460-2075.1993.tb05877.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Nielsen S., DiGiovanni S. R., Christensen E. I., Knepper M. A., Harris H. W. Cellular and subcellular immunolocalization of vasopressin-regulated water channel in rat kidney. Proc Natl Acad Sci U S A. 1993 Dec 15;90(24):11663–11667. doi: 10.1073/pnas.90.24.11663. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Nielsen S., Smith B. L., Christensen E. I., Knepper M. A., Agre P. CHIP28 water channels are localized in constitutively water-permeable segments of the nephron. J Cell Biol. 1993 Jan;120(2):371–383. doi: 10.1083/jcb.120.2.371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Opperman C. H., Taylor C. G., Conkling M. A. Root-knot nematode--directed expression of a plant root--specific gene. Science. 1994 Jan 14;263(5144):221–223. doi: 10.1126/science.263.5144.221. [DOI] [PubMed] [Google Scholar]
  10. Preston G. M., Carroll T. P., Guggino W. B., Agre P. Appearance of water channels in Xenopus oocytes expressing red cell CHIP28 protein. Science. 1992 Apr 17;256(5055):385–387. doi: 10.1126/science.256.5055.385. [DOI] [PubMed] [Google Scholar]
  11. Riederer B. M. Antigen preservation tests for immunocytochemical detection of cytoskeletal proteins: influence of aldehyde fixatives. J Histochem Cytochem. 1989 May;37(5):675–681. doi: 10.1177/37.5.2495321. [DOI] [PubMed] [Google Scholar]
  12. Sabolić I., Katsura T., Verbavatz J. M., Brown D. The AQP2 water channel: effect of vasopressin treatment, microtubule disruption, and distribution in neonatal rats. J Membr Biol. 1995 Feb;143(3):165–175. doi: 10.1007/BF00233445. [DOI] [PubMed] [Google Scholar]
  13. Yamada S., Katsuhara M., Kelly W. B., Michalowski C. B., Bohnert H. J. A family of transcripts encoding water channel proteins: tissue-specific expression in the common ice plant. Plant Cell. 1995 Aug;7(8):1129–1142. doi: 10.1105/tpc.7.8.1129. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES