Abstract
NaCl-induced plasma membrane H+-ATPase gene expression, which occurs in roots and fully expanded leaves of the halophyte Atriplex nummularia L. (X. Niu, M.L. Narasimhan, R.A. Salzman, R.A. Bressan, P.M. Hasegawa [1993] Plant Physiol 103: 713-718), has been differentially localized to specific tissues using in situ RNA hybridization techniques. Twenty-four-hour exposure of plants to 400 mM NaCl resulted in substantial accumulation of H+ pump message in the epidermis of the root tip and the endodermis of the root elongation/differentiation zone. In expanded leaves, NaCl induction of plasma membrane H+-ATPase message accumulation was localized to bundle-sheath cells. Ultrastructural analyses indicated that significant cytological adaptations in root cells included plasmolysis that is accompanied by plasma membrane invaginations, formation of Hechtian strands and vesiculation, and vacuolation. These results identify specific tissues that are involved in the regulation of Na+ and Cl- uptake into different organs of the halophyte A. nummularia and provide evidence of the intercellular and interorgan coordination that occurs in the mediation of NaCl adaptation.
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Selected References
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- Binzel M. L., Hess F. D., Bressan R. A., Hasegawa P. M. Intracellular compartmentation of ions in salt adapted tobacco cells. Plant Physiol. 1988 Feb;86(2):607–614. doi: 10.1104/pp.86.2.607. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Braun Y., Hassidim M., Lerner H. R., Reinhold L. Studies on H-Translocating ATPases in Plants of Varying Resistance to Salinity : I. Salinity during Growth Modulates the Proton Pump in the Halophyte Atriplex nummularia. Plant Physiol. 1986 Aug;81(4):1050–1056. doi: 10.1104/pp.81.4.1050. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Niu X., Bressan R. A., Hasegawa P. M., Pardo J. M. Ion Homeostasis in NaCl Stress Environments. Plant Physiol. 1995 Nov;109(3):735–742. doi: 10.1104/pp.109.3.735. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Niu X., Zhu J. K., Narasimhan M. L., Bressan R. A., Hasegawa P. M. Plasma-membrane H(+)-ATPase gene expression is regulated by NaCl in cells of the halophyte Atriplex nummularia L. Planta. 1993;190(4):433–438. doi: 10.1007/BF00224780. [DOI] [PubMed] [Google Scholar]
- Pak J. Y., Fukuhara T., Nitta T. Discrete subcellular localization of membrane-bound ATPase activity in marine angiosperms and marine algae. Planta. 1995;196(1):15–22. doi: 10.1007/BF00193212. [DOI] [PubMed] [Google Scholar]