Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1967 Jul;2(4):377–398.

The production of immunoglobulins by human peripheral blood lymphocytes in vitro

Carolyn S Ripps, K Hirschhorn
PMCID: PMC1578853  PMID: 4167746

Abstract

We have demonstrated that the peripheral blood lymphocytes of normal humans show an increased production of immunoglobulins in vitro after non-specific stimulation by phytohaemagglutinin (PHA). The methods used include immunofluorescence and immune co-precipitation. Specific antigens to which the donor has been sensitized produce a similar effect in a smaller number of cells. The cells of persons with defects of immunoglobulin production can be stimulated by PHA to enlarge and divide, but their immunoglobulin content is abnormal and reflects the pattern of the circulating serum globulins. Preliminary results suggest that a portion of the immunoglobulin produced after either specific or non-specific stimulation of cells from sensitized donors behaves like specific antibody.

Full text

PDF
377

Images in this article

380Fig. 1
on p.380
384-1p384-1-a
on p.384-1

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ATTARDI G., COHN M., HORIBATA K., LENNOX E. S. Symposium on the biology of cells modified by viruses or antigens. II. On the analysis of antibody synthesis at the cellular level. Bacteriol Rev. 1959 Dec;23(4):213–223. doi: 10.1128/br.23.4.213-223.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ATTARDI G., COHN M., HORIBITA K., LENNOX E. S. ANTIBODY FORMATION BY RABBIT LYMPH NODE CELLS. V. CELLULAR HETEROGENEITY IN THE PRODUCTION OF ANTIBODY TO T5. J Immunol. 1964 Jul;93:94–95. [PubMed] [Google Scholar]
  3. BACH F., HIRSCHHORN K. GAMMA-GLOBULIN PRODUCTION BY HUMAN LYMPHOCYTES IN VITRO. Exp Cell Res. 1963 Dec;32:592–595. doi: 10.1016/0014-4827(63)90197-6. [DOI] [PubMed] [Google Scholar]
  4. BRITTIN G. M., TANAKA Y., BRECHER G. Intranuclear inclusions in multiple myeloma and macroglobulinemia. Blood. 1963 Mar;21:335–351. [PubMed] [Google Scholar]
  5. BUCKTON K. E., JACOBS P. A., COURT BROWN W. M., DOLL R. A study of the chromosome damage persisting after x-ray therapy for ankylosing spondylitis. Lancet. 1962 Oct 6;2(7258):676–682. doi: 10.1016/s0140-6736(62)90500-7. [DOI] [PubMed] [Google Scholar]
  6. Bernier G. M., Cebra J. J. Frequency distribution of alpha, gamma, kappa and lambda polypeptide chains in human lymphoid tissues. J Immunol. 1965 Aug;95(2):246–253. [PubMed] [Google Scholar]
  7. CHIAPPINO G., PERNIS B. DEMONSTRATION WITH IMMUNOFLUORESCENCE OF 19S MACROGLOBULINS AND 7S GAMMA GLOBULINS IN DIFFERENT CELLS OF THE HUMAN SPLEEN. Pathol Microbiol (Basel) 1964;27:8–15. doi: 10.1159/000161448. [DOI] [PubMed] [Google Scholar]
  8. CRUMPTON M. J., WILKINSON J. M. AMINO ACID COMPOSITIONS OF HUMAN AND RABBIT GAMMA-GLOBULINS AND OF THE FRAGMENTS PRODUCED BY REDUCTION. Biochem J. 1963 Aug;88:228–234. doi: 10.1042/bj0880228. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. FELLNER M. J., BAER R. L. ANAPHYLACTIC REACTION TO TETRACYCLINE IN A PENICILLIN-ALLERGIC PATIENT: IMMUNOLOGIC STUDIES. JAMA. 1965 Jun 14;192:997–998. doi: 10.1001/jama.1965.03080240067023. [DOI] [PubMed] [Google Scholar]
  10. FORBES I. J. SPECIFIC AND NON-SPECIFIC STIMULATION OF ANTIBODY SYNTHESIS BY HUMAN LEUCOCYTES IN VITRO. Lancet. 1965 Jan 23;1(7378):198–199. doi: 10.1016/s0140-6736(65)90979-7. [DOI] [PubMed] [Google Scholar]
  11. Forbes I. J., Turner K. J. Synthesis of protein by human lymphocytes in vitro. I. Clinical studies. Australas Ann Med. 1965 Nov;14(4):304–310. doi: 10.1111/imj.1965.14.4.304. [DOI] [PubMed] [Google Scholar]
  12. Gamble C. N., Cutting H. O. The production of gamma G-globulin by lymphocytes in chronic lymphocytic leukemia. Immunocytologic investigation of a case. Blood. 1966 Feb;27(2):187–198. [PubMed] [Google Scholar]
  13. Gell P. G., Sell S. Studies on rabbit lymphocytes in vitro. II. Induction of blast transformation with antisera to six IgG allotypes and summation with mixtures of antisera to different allotypes. J Exp Med. 1965 Oct 1;122(4):813–821. doi: 10.1084/jem.122.4.813. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. HULLIGER L., SORKIN E. Synthesis of antibodies by blood leucocytes of the rabbit. Nature. 1963 Apr 20;198:299–299. doi: 10.1038/198299a0. [DOI] [PubMed] [Google Scholar]
  15. Hiramoto R. N., Hamlin M. Detection of two antibodies in single plasma cells by the paired fluorescence technique. J Immunol. 1965 Aug;95(2):214–224. [PubMed] [Google Scholar]
  16. KEARNEY R., HALLIDAY W. J. ENUMERATION OF ANTIBODY-FORMING CELLS IN THE PERIPHERAL BLOOD OF IMMUNIZED RABBITS. J Immunol. 1965 Jul;95:109–112. [PubMed] [Google Scholar]
  17. LANDY M., SANDERSON R. P., BERNSTEIN M. T., JACKSON A. L. ANTIBODY PRODUCTION BY LEUCOCYTES IN PERIPHERAL BLOOD. Nature. 1964 Dec 26;204:1320–1321. doi: 10.1038/2041320a0. [DOI] [PubMed] [Google Scholar]
  18. LYON M. F. Sex chromatin and gene action in the mammalian X-chromosome. Am J Hum Genet. 1962 Jun;14:135–148. [PMC free article] [PubMed] [Google Scholar]
  19. Levine B. B., Fellner M. J., Levytska V. Benzylpenicilloyl specific serum antibodies to penicillin in man. I. Development of a sensitive hemagglutination assay method and haptenic specificities of antibodies. J Immunol. 1966 Apr;96(4):707–718. [PubMed] [Google Scholar]
  20. Levine B. B., Fellner M. J., Levytska V., Franklin E. C., Alisberg N. Benzylpenicilloyl-specific serum antibodies to penicillin in man. II. Sensitivity of the hemagglutination assay method, molecular classes of the antibodies detected, and antibody titers of randomly selected patients. J Immunol. 1966 Apr;96(4):719–726. [PubMed] [Google Scholar]
  21. MELLORS R. C., KORNGOLD L. THE CELLULAR ORIGIN OF HUMAN IMMUNOGLOBULINS (GAMMA-2, GAMMA-1M, GAMMA-1A). J Exp Med. 1963 Sep 1;118:387–396. doi: 10.1084/jem.118.3.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Marinkovich V. A., Baluda M. A. In vitro synthesis of gamma M-like globulin by various chick embryonic cells. Immunology. 1966 Apr;10(4):383–397. [PMC free article] [PubMed] [Google Scholar]
  23. Möller G. 19S antibody production against soluble lipopolysaccharide antigens by individual lymphoid cells in vitro. Nature. 1965 Sep 11;207(5002):1166–1168. doi: 10.1038/2071166a0. [DOI] [PubMed] [Google Scholar]
  24. NORMAN A., SASAKI M. S., OTTOMAN R. E., FINGERHUT A. G. LYMPHOCYTE LIFETIME IN WOMEN. Science. 1965 Feb 12;147(3659):745–745. doi: 10.1126/science.147.3659.745. [DOI] [PubMed] [Google Scholar]
  25. NOSSAL G. J., MAKELA O. Elaboration of antibodies by single cells. Annu Rev Microbiol. 1962;16:53–74. doi: 10.1146/annurev.mi.16.100162.000413. [DOI] [PubMed] [Google Scholar]
  26. Nowell P. C. Unstable chromosome changes in tuberculin-stimulated leukocyte cultures from irradiated patients. Evidence for immunologically committed, long-lived lymphocytes in human blood. Blood. 1965 Dec;26(6):798–804. [PubMed] [Google Scholar]
  27. PERNIS B., CHIAPPINO G. IDENTIFICATION IN HUMAN LYMPHOID TISSUES OF CELLS THAT PRODUCE GROUP 1 OR GROUP 2 GAMMA-GLOBULINS. Immunology. 1964 Sep;7:500–506. [PMC free article] [PubMed] [Google Scholar]
  28. Parenti F., Franceschini P., Forti G., Cepellini R. The effect of phytohaemagglutinin on the metabolism and gamma-globulin synthesis of human lymphocytes. Biochim Biophys Acta. 1966 Jul 20;123(1):181–187. doi: 10.1016/0005-2787(66)90171-7. [DOI] [PubMed] [Google Scholar]
  29. Pernis B., Chiappino G., Kelus A. S., Gell P. G. Cellular localization of immunoglobulins with different allotypic specificities in rabbit lymphoid tissues. J Exp Med. 1965 Nov 1;122(5):853–876. doi: 10.1084/jem.122.5.853. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Pogo B. G., Allfrey V. G., Mirsky A. E. RNA synthesis and histone acetylation during the course of gene activation in lymphocytes. Proc Natl Acad Sci U S A. 1966 Apr;55(4):805–812. doi: 10.1073/pnas.55.4.805. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Sell S., Rowe D. S., Gell P. G. Studies on rabbit lymphocytes in vitro. 3. Proteins, RNA, and DNA synthesis by lymphocyte cultures after stimulation with phytohaemagglutinin, with staphylococcal filtrate, with antiallotype serum, and with heterologous antiserum to rabbit whole serum. J Exp Med. 1965 Oct 1;122(4):823–839. doi: 10.1084/jem.122.4.823. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Turner K. J., Forbes I. J. Synthesis of proteins by human leukocytes in vitro. II. Chemical characterization. J Immunol. 1966 Jun;96(6):926–935. [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES