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. 1968 Mar;3(3):213–226.

Particles resembling murine leukaemia virus in New Zealand Black mice

Patricia R Prosser
PMCID: PMC1578886  PMID: 4298059

Abstract

Particles which morphologically resembled murine leukaemia virus were detected by electron microscopy in the tissues (spleen, thymus, inguinal lymph nodes, bone marrow or pancreas) but not in serum or plasma pellets of untreated conventional New Zealand Black (NZB) mice aged 1–82 weeks. They were also found in the corresponding tissues of NZB mice which had been thymectomized shortly after birth. The presence of similar particles in the spleen, thymus or pancreas of conventional NZB embryos and, additionally, in the lymph nodes of NZB mice which had originally been introduced into a germ-free environment by Caesarian section and fostering on germ-free mice of another strain, suggests that the virus is transmitted `vertically' through the germ cells or placenta. Preliminary investigations showed similar particles in the organs of conventional F1 (NZB × NZW) hybrid and New Zealand White (NZW) mice.

Large numbers of particles also resembling murine leukaemia virus were found in the spleen and in plasma or serum pellets of young conventional NZB mice which had developed reticulum cell neoplasia following serial passage of lymphoid cell suspensions from ageing conventional NZB donors.

The possible relationship of these particles to the autoimmune reactions and malignant changes which occur spontaneously in conventional NZB mice is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BERNHARD W. The detection and study of tumor viruses with the electron microscope. Cancer Res. 1960 Jun;20:712–727. [PubMed] [Google Scholar]
  2. BIELSCHOWSKY M., BIELSCHOWSKY F. OBSERVATIONS ON NZB/B1 MICE; DIFFERENTIAL FERTILITY IN RECIPROCAL CROSSES AND THE TRANSMISSION OF THE AUTO-IMMUNE HAEMOLYTIC ANAEMIA TO NZB/B1 X NZC/B1 HYBRIDS. Aust J Exp Biol Med Sci. 1964 Aug;42:561–568. [PubMed] [Google Scholar]
  3. Chapman A. L., Bopp W., Brightwell S., Nielson A., Werder A. Distribution of virus-like particles in the lymphatic tissues of "nonleukemic" CFW-w conventional mice. Proc Soc Exp Biol Med. 1966 Dec;123(3):742–746. doi: 10.3181/00379727-123-31592. [DOI] [PubMed] [Google Scholar]
  4. DALTON A. J., HAGUENAU F., MOLONEY J. B. Morphology of particles associated with murine leukemia as revealed by negative staining: preliminary report. J Natl Cancer Inst. 1962 Dec;29:1177–1179. [PubMed] [Google Scholar]
  5. East J., De Sousa M. A., Prosser P. R., Jaquet H. Malignant changes in New Zealand black mice. Clin Exp Immunol. 1967 Jul;2(4):427–443. [PMC free article] [PubMed] [Google Scholar]
  6. East J., De Sousa M. A. The thymus autoimmunity and malignancy in New Zealand black mice. Natl Cancer Inst Monogr. 1966 Sep;22:605–616. [PubMed] [Google Scholar]
  7. East J., Prosser P. R. Autoimmunity and malignancy in New Zealand black mice. Proc R Soc Med. 1967 Aug;60(8):823–825. [PMC free article] [PubMed] [Google Scholar]
  8. East J., Prosser P. R., Holborow E. J., Jaquet H. Autoimmune reactions and virus-like particles in germ-free NZB mice. Lancet. 1967 Apr 8;1(7493):755–757. doi: 10.1016/s0140-6736(67)91368-2. [DOI] [PubMed] [Google Scholar]
  9. East J., de Sousa M. A., Parrott D. M. Immunopathology of New Zealand black (NZB) mice. Transplantation. 1965 Nov;3(6):711–729. doi: 10.1097/00007890-196511000-00003. [DOI] [PubMed] [Google Scholar]
  10. Feldman D. G., Gross L. Electron microscopic study of the distribution of the mouse leukemia virus (gross) in organs of mice and rats with virus-induced leukemia. Cancer Res. 1966 Mar;26(3):412–426. [PubMed] [Google Scholar]
  11. HELYER B. J., HOWIE J. B. Renal disease associated with positive lupus erythematosus tests in a cross-bred strain of mice. Nature. 1963 Jan 12;197:197–197. doi: 10.1038/197197a0. [DOI] [PubMed] [Google Scholar]
  12. HELYER B. J., HOWIE J. B. Spontaneous auto-immune disease in NZB/BL mice. Br J Haematol. 1963 Apr;9:119–131. doi: 10.1111/j.1365-2141.1963.tb05450.x. [DOI] [PubMed] [Google Scholar]
  13. HOLMES M. C., BURNET F. M. THE NATURAL HISTORY OF AUTOIMMUNE DISEASE IN NZB MICE. A COMPARISON WITH THE PATTERN OF HUMAN AUTOIMMUNE MANIFESTATIONS. Ann Intern Med. 1963 Sep;59:265–276. doi: 10.7326/0003-4819-59-3-265. [DOI] [PubMed] [Google Scholar]
  14. Hicks J. D., Burnet F. M. Renal lesions in the "auto-immune" mouse strains NZB and F1 NZBxNZW. J Pathol Bacteriol. 1966 Apr;91(2):467–476. doi: 10.1002/path.1700910221. [DOI] [PubMed] [Google Scholar]
  15. Hollman K. H., Verley J. M. Elaboration de particules virales de type B dans le thymus de la souris. Etude au microscope électronique. Z Zellforsch Mikrosk Anat. 1967;78(1):47–53. [PubMed] [Google Scholar]
  16. Hollmann K. H., Verley J. M. Présence de particules virales dans les organes de la souris NZB. C R Acad Sci Hebd Seances Acad Sci D. 1967 Feb 6;264(6):871–873. [PubMed] [Google Scholar]
  17. KARNOVSKY M. J. Simple methods for "staining with lead" at high pH in electron microscopy. J Biophys Biochem Cytol. 1961 Dec;11:729–732. doi: 10.1083/jcb.11.3.729. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kajima M., Pollard M. Detection of viruslike particles in germ-free mice. J Bacteriol. 1965 Nov;90(5):1448–1454. doi: 10.1128/jb.90.5.1448-1454.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. MELLORS R. C. AUTOIMMUNE DISEASE IN NZB/BL MICE. I. PATHOLOGY AND PATHOGENESIS OF A MODEL SYSTEM OF SPONTANEOUS GLOMERULONEPHRITIS. J Exp Med. 1965 Jul 1;122:25–40. doi: 10.1084/jem.122.1.25. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Mellors R. C. Autoimmune disease in NZB/BL mice. 3. Induction of membranous glomerulonephritis in young mice by the transplantation of spleen cells from old mice. J Exp Med. 1966 Jun 1;123(6):1025–1034. doi: 10.1084/jem.123.6.1025. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Mellors R. C., Huang C. Y. Immunopathology of NZB/BL mice. V. Viruslike (filtrable) agent separable from lymphoma cells and identifiable by electron microscopy. J Exp Med. 1966 Dec 1;124(6):1031–1038. doi: 10.1084/jem.124.6.1031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Mellors R. C., Huang C. Y. Immunopathology of NZB/BL mice. VI. Virus separable from spleen and pathogenic for Swiss mice. J Exp Med. 1967 Jul 1;126(1):53–62. doi: 10.1084/jem.126.1.53. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. NORINS L. C., HOLMES M. C. GLOBULINS OF NZB MOUSE ERYTHROCYTES. J Immunol. 1964 Dec;93:897–901. [PubMed] [Google Scholar]
  24. PALADE G. E. A study of fixation for electron microscopy. J Exp Med. 1952 Mar;95(3):285–298. doi: 10.1084/jem.95.3.285. [DOI] [PMC free article] [PubMed] [Google Scholar]

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