Abstract
Hypocotyls from etiolated cucumber (Cucumis sativus L.) seedlings were gently abraded at their epidermal surface, and cut segments were used to study the rapid and transient elicitation of H2O2 by ergosterol, chitosan, mastoparan, and a polymeric fungal elicitor. Freshly abraded segments were only barely competent for any H2O2 production, but they developed this competence subsequent to abrasion. This process was enhanced by 2,6-dichloroisonicotinic acid and salicylic acid, which induced acquired resistance to fungal penetration in the epidermal cells. Enhancement of competence induction by salicylic acid was also evident for spontaneous H2O2 production and differed in degree for the various elicitors, indicating that mainly the enzyme complex producing H2O2, but also other components of the elicitation system, improved. Ergosterol, chitosan, and fungal elicitor also rendered the segments refractory to a second stimulation by the same compound, whereas mastoparan was inactive in this respect. The four elicitors also differed markedly in their ability to diminish or enhance H2O2 production by a second treatment with a different elicitor, indicating that several sites of the H2O2 elicitation system are subject to short-term regulation.
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Selected References
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- Chen Z., Klessig D. F. Identification of a soluble salicylic acid-binding protein that may function in signal transduction in the plant disease-resistance response. Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):8179–8183. doi: 10.1073/pnas.88.18.8179. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Conrath U., Chen Z., Ricigliano J. R., Klessig D. F. Two inducers of plant defense responses, 2,6-dichloroisonicotinec acid and salicylic acid, inhibit catalase activity in tobacco. Proc Natl Acad Sci U S A. 1995 Aug 1;92(16):7143–7147. doi: 10.1073/pnas.92.16.7143. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fauth M., Merten A., Hahn M. G., Jeblick W., Kauss H. Competence for Elicitation of H2O2 in Hypocotyls of Cucumber Is Induced by Breaching the Cuticle and Is Enhanced by Salicylic Acid. Plant Physiol. 1996 Feb;110(2):347–354. doi: 10.1104/pp.110.2.347. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Granado J., Felix G., Boller T. Perception of Fungal Sterols in Plants (Subnanomolar Concentrations of Ergosterol Elicit Extracellular Alkalinization in Tomato Cells). Plant Physiol. 1995 Feb;107(2):485–490. doi: 10.1104/pp.107.2.485. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hadwiger L. A., Ogawa T., Kuyama H. Chitosan polymer sizes effective in inducing phytoalexin accumulation and fungal suppression are verified with synthesized oligomers. Mol Plant Microbe Interact. 1994 Jul-Aug;7(4):531–533. doi: 10.1094/mpmi-7-0531. [DOI] [PubMed] [Google Scholar]
- Kauss H., Jeblick W. Pretreatment of Parsley Suspension Cultures with Salicylic Acid Enhances Spontaneous and Elicited Production of H2O2. Plant Physiol. 1995 Jul;108(3):1171–1178. doi: 10.1104/pp.108.3.1171. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Klessig D. F., Malamy J. The salicylic acid signal in plants. Plant Mol Biol. 1994 Dec;26(5):1439–1458. doi: 10.1007/BF00016484. [DOI] [PubMed] [Google Scholar]
- Kogel K. H., Beckhove U., Dreschers J., Munch S., Romme Y. Acquired Resistance in Barley (The Resistance Mechanism Induced by 2,6-Dichloroisonicotinic Acid Is a Phenocopy of a Genetically Based Mechanism Governing Race-Specific Powdery Mildew Resistance). Plant Physiol. 1994 Dec;106(4):1269–1277. doi: 10.1104/pp.106.4.1269. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kolattukudy P. E., Rogers L. M., Li D., Hwang C. S., Flaishman M. A. Surface signaling in pathogenesis. Proc Natl Acad Sci U S A. 1995 May 9;92(10):4080–4087. doi: 10.1073/pnas.92.10.4080. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mehdy M. C. Active Oxygen Species in Plant Defense against Pathogens. Plant Physiol. 1994 Jun;105(2):467–472. doi: 10.1104/pp.105.2.467. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ryals J., Uknes S., Ward E. Systemic Acquired Resistance. Plant Physiol. 1994 Apr;104(4):1109–1112. doi: 10.1104/pp.104.4.1109. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vogelstein B., Kinzler K. W. Has the breast cancer gene been found? Cell. 1994 Oct 7;79(1):1–3. doi: 10.1016/0092-8674(94)90393-x. [DOI] [PubMed] [Google Scholar]