Abstract
In this paper the preparation and examination of pure antisera against immunoglobulins is described. The results of serological studies obtained with pure anti-IgG, anti-IgM and anti-IgA sera as well as an anti-complement serum and the blood of four patients with acquired haemolytic anaemia are discussed. The presence of incomplete warm autoantibodies belonging to the G, M and A classes of immunoglobulins was demonstrated. Further, evidence was obtained that warm haemolysins are a separate antibody category independent of the presence of incomplete warm autoantibodies.
A new serological classification of the autoimmune haemolytic anaemias based on these findings is given.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Gerbal A., Homberg J. C., Rochant H., Liberge G., Delarue F., Salmon C. Nouvelle classification immunologique des anémies hémolytiques avec auto-anticorps. Nouv Rev Fr Hematol. 1967 May-Jun;7(3):401–406. [PubMed] [Google Scholar]
- Jeannet M. Observations sur la spécificité du test de Coombs direct dans les anémies hémolytiques "auto-immunes". Schweiz Med Wochenschr. 1965 Oct 23;95(43):1442–1444. [PubMed] [Google Scholar]
- ROTHFIELD N. F., FRANGIONE B., FRANKLIN E. C. SLOWLY SEDIMENTING MERCAPTOETHANOL-RESISTANT ANTINUCLEAR FACTORS RELATED ANTIGENICALLY TO M IMMUNOGLOBULINS (GAMMA-1M-GLOBULIN) IN PATIENTS WITH SYSTEMIC LUPUS ERYTHEMATOSUS. J Clin Invest. 1965 Jan;44:62–72. doi: 10.1172/JCI105127. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RUBIN H. Antibody elution from red blood cells. J Clin Pathol. 1963 Jan;16:70–73. doi: 10.1136/jcp.16.1.70. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stobo J. D., Tomasi T. B. A Low Molecular Weight Immunoglobulin Antigenically Related to 19 S IgM. J Clin Invest. 1967 Aug;46(8):1329–1337. doi: 10.1172/JCI105625. [DOI] [PMC free article] [PubMed] [Google Scholar]