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. 1968 Nov;3(9):877–888.

An assay for adjuvanticity

D W Dresser
PMCID: PMC1579011  PMID: 4179956

Abstract

Adult mice injected with an adequate amount of a non-immunogenic antigen progress to a specific state of immunological paralysis, unless a substance with `extrinsic' adjuvanticity is injected before the induction of paralysis is completed. Consequently incipiently paralysed mice can be used to assay substances for adjuvanticity. Conventional adjuvants such as Freund's adjuvant and pertussis possess adjuvanticity; other substances with varying degrees of adjuvanticity are listed in the tables. It has been shown that the adjuvanticity effect of an injection of pertussis lasts for only a few days, although the effect of such an injection of pertussis on phagocytosis of carbon particles does not reach a maximum until 2 weeks after the injection. The dose-effectiveness of alum precipitated (highly phagocytosable) bovine γ-globulin was greatly increased by the intraperitoneal injection of pertussis. The evidence is considered to be incompatible with increased phagocytosis being either an essential factor in the role of pertussis as a conventional adjuvant, or in the adjuvanticity effect of pertussis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allison A. C., Harington J. S., Birbeck M. An examination of the cytotoxic effects of silica on macrophages. J Exp Med. 1966 Aug 1;124(2):141–154. doi: 10.1084/jem.124.2.141. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DRESSER D. W. Acquired immunological tolerance to a fraction of bovine gamma globulin. Immunology. 1961 Jan;4:13–23. [PMC free article] [PubMed] [Google Scholar]
  3. DRESSER D. W. Effectiveness of lipid and lipidophilic substances as adjuvants. Nature. 1961 Sep 16;191:1169–1171. doi: 10.1038/1911169a0. [DOI] [PubMed] [Google Scholar]
  4. DRESSER D. W. SPECIFIC INHIBITION OF ANTIBODY PRODUCTION. III. APPARENT CHANGES IN THE HALF-LIFE OF BOVINE GAMMA GLOBULIN IN PARALYSED MICE. Immunology. 1963 Jul;6:345–355. [PMC free article] [PubMed] [Google Scholar]
  5. DRESSER D. W. Specific inhibition of antibody production. I. Protein-over loading paralysis. Immunology. 1962 Jan;5:161–168. [PMC free article] [PubMed] [Google Scholar]
  6. DRESSER D. W. Specific inhibition of antibody production. II. Paralysis induced in adult mice by small quantities of protein antigen. Immunology. 1962 May;5:378–388. [PMC free article] [PubMed] [Google Scholar]
  7. Dresser D. W. Adjuvanticity of vitamin A. Nature. 1968 Feb 10;217(5128):527–529. doi: 10.1038/217527a0. [DOI] [PubMed] [Google Scholar]
  8. Dresser D. W. Specific inhibition of antibody production. IV. Standardization of the antigen-elimination test; immunological paralysis of mice previously immunized. Immunology. 1965 Sep;9(3):261–273. [PMC free article] [PubMed] [Google Scholar]
  9. Gall D. The adjuvant activity of aliphatic nitrogenous bases. Immunology. 1966 Oct;11(4):369–386. [PMC free article] [PubMed] [Google Scholar]
  10. Jansz A., Flad H. D., Koffler D., Miescher P. A. The effect of vitamin A on experimental immune thyroiditis. Int Arch Allergy Appl Immunol. 1967;31(1):69–77. doi: 10.1159/000229855. [DOI] [PubMed] [Google Scholar]
  11. MERRITT K., JOHNSON A. G. STUDIES ON THE ADJUVANT ACTION OF BACTERIAL ENDOTOXINS ON ANTIBODY FORMATION. VI. ENHANCEMENT OF ANTIBODY FORMATION BY NUCLEIC ACIDS. J Immunol. 1965 Mar;94:416–422. [PubMed] [Google Scholar]
  12. MORSE S. I. STUDIES ON THE LYMPHOCYTOSIS INDUCED IN MICE BY BORDETELLA PERTUSSIS. J Exp Med. 1965 Jan 1;121:49–68. doi: 10.1084/jem.121.1.49. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McFARLANE A. S. Efficient trace-labelling of proteins with iodine. Nature. 1958 Jul 5;182(4627):53–53. doi: 10.1038/182053a0. [DOI] [PubMed] [Google Scholar]
  14. Morse S. I., Riester S. K. Studies on the leukocytosis and lymphocytosis induced by Bordetella pertussis. I. Radioautographic analysis of the circulating cells in mice undergoing pertussis-induced hyperleukocytosis. J Exp Med. 1967 Mar 1;125(3):401–408. doi: 10.1084/jem.125.3.401. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Morse S. I., Riester S. K. Studies on the leukocytosis and lymphocytosis induced by Bordetella pertussis. II. The effect of pertussis vaccine on the thoracic duct lymph and lymphocytes of mice. J Exp Med. 1967 Apr 1;125(4):619–628. doi: 10.1084/jem.125.4.619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Pieroni R. E., Levine L. Adjuvant principle of pertussis vaccine in the mouse. Nature. 1966 Sep 24;211(5056):1419–1420. doi: 10.1038/2111419a0. [DOI] [PubMed] [Google Scholar]

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