Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1969 Jan;4(1):55–63.

Apparent dissociation of antigen responsiveness and antibody production by human small lymphocytes in culture

M J Simons, M G Fitzgerald
PMCID: PMC1579049  PMID: 5786813

Abstract

Human peripheral blood small lymphocytes include cells which carry specific immunological memory for previously experienced antigens. Even in the absence of other cell types, these memory cells respond to antigen by DNA synthesis, blast transformation and mitosis. Utilizing the technique of bacterial adherence, evidence of antibody production by leucocytes from immunized individuals was readily obtained, whereas no evidence of antibody production by antigen stimulated lymphoblastoid cells was found.

Full text

PDF
55

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adler F. L., Fishman M., Dray S. Antibody formation initiated in vitro. 3. Antibody formation and allotypic specificity directed by ribonucleic acid from peritoneal exudate cells. J Immunol. 1966 Oct;97(4):554–558. [PubMed] [Google Scholar]
  2. BACH F., HIRSCHHORN K. GAMMA-GLOBULIN PRODUCTION BY HUMAN LYMPHOCYTES IN VITRO. Exp Cell Res. 1963 Dec;32:592–595. doi: 10.1016/0014-4827(63)90197-6. [DOI] [PubMed] [Google Scholar]
  3. BALFOUR B. M., COOPER E. H., ALPEN E. L. MORPHOLOGICAL AND KINETIC STUDIES ON ANTIBODY-PRODUCING CELLS IN RAT LYMPH NODES. Immunology. 1965 Mar;8:230–244. [PMC free article] [PubMed] [Google Scholar]
  4. BARTFELD H., JULIAR J. F. "IMMUNOLOGICAL ORGANISATION" AND ACTIVITY OF HUMAN PERIPHERAL WHITE-BLOOD-CELL CULTURES. Lancet. 1964 Oct 10;2(7363):767–769. doi: 10.1016/s0140-6736(64)90555-0. [DOI] [PubMed] [Google Scholar]
  5. COWLING D. C., QUAGLINO D., DAVIDSON E. CHANGES INDUCED BY TUBERCULIN IN LEUCOCYTE CULTURES. Lancet. 1963 Nov 23;2(7317):1091–1094. doi: 10.1016/s0140-6736(63)92860-5. [DOI] [PubMed] [Google Scholar]
  6. COWLING D. C., QUAGLINO D. EFFECT OF SOME ANTIGENS ON LEUCOCYTE CULTURES. J Pathol Bacteriol. 1965 Jan;89:63–70. doi: 10.1002/path.1700890108. [DOI] [PubMed] [Google Scholar]
  7. Caron G. A. Lymphocyte transformation induced by different doses of tuberculin as measured by tritiated thymidine uptake. Int Arch Allergy Appl Immunol. 1966;30(4):331–334. doi: 10.1159/000229817. [DOI] [PubMed] [Google Scholar]
  8. Coulson A. S., Chalmers D. G. Response of human blood lymphocytes to tuberculin PPD in tissue culture. Immunology. 1967 Apr;12(4):417–429. [PMC free article] [PubMed] [Google Scholar]
  9. DUTTON R. W., EADY J. D. AN IN VITRO SYSTEM FOR THE STUDY OF THE MECHANISM OF ANTIGENIC STIMULATION IN THE SECONDARY RESPONSE. Immunology. 1964 Jan;7:40–53. [PMC free article] [PubMed] [Google Scholar]
  10. Davies A. J., Leuchars E., Wallis V., Koller P. C. The mitotic response of thymus-derived cells to antigenic stimulus. Transplantation. 1966 Jul;4(4):438–451. doi: 10.1097/00007890-196607000-00008. [DOI] [PubMed] [Google Scholar]
  11. Diener E., Armstrong W. D. Induction of antibody formation and tolerance in vitro to a purified protein antigen. Lancet. 1967 Dec 16;2(7529):1281–1285. doi: 10.1016/s0140-6736(67)90394-7. [DOI] [PubMed] [Google Scholar]
  12. FORBES I. J. SPECIFIC AND NON-SPECIFIC STIMULATION OF ANTIBODY SYNTHESIS BY HUMAN LEUCOCYTES IN VITRO. Lancet. 1965 Jan 23;1(7378):198–199. doi: 10.1016/s0140-6736(65)90979-7. [DOI] [PubMed] [Google Scholar]
  13. HIRSCHHORN K., BACH F., KOLODNY R. L., FIRSCHEIN I. L., HASHEM N. IMMUNE RESPONSE AND MITOSIS OF HUMAN PERIPHERAL BLOOD LYMPHOCYTES IN VITRO. Science. 1963 Nov 29;142(3596):1185–1187. doi: 10.1126/science.142.3596.1185. [DOI] [PubMed] [Google Scholar]
  14. Hartog M., Cline M. J., Grodsky G. M. The response of human leucocyte cultures to stimulation by tuberculin and phytohaemagglutinin measured by the uptake of radioactive thymidine. Clin Exp Immunol. 1967 Mar;2(2):217–228. [PMC free article] [PubMed] [Google Scholar]
  15. LING N. R., SPICER E., JAMES K., WILLIAMSON N. THE ACTIVATION OF HUMAN PERIPHERAL LYMPHOCYTES BY PRODUCTS OF STAPHYLOCOCCI. Br J Haematol. 1965 Jul;11:421–431. doi: 10.1111/j.1365-2141.1965.tb06604.x. [DOI] [PubMed] [Google Scholar]
  16. Lobb N., Curtain C. C., Kidson C. Regulatory genes controlling the synthesis of heavy chains of human immunoglobulin-G. Nature. 1967 May 20;214(5090):783–785. doi: 10.1038/214783a0. [DOI] [PubMed] [Google Scholar]
  17. MACLAURIN B. P. ROSETTE FORMATION IN LYMPHOCYTE CULTURES. Lancet. 1965 Jun 12;1(7398):1278–1278. doi: 10.1016/s0140-6736(65)91936-7. [DOI] [PubMed] [Google Scholar]
  18. MAKELA O., NOSSAL G. J. Bacterial adherence: a method for detecting antibody production by single cells. J Immunol. 1961 Oct;87:447–456. [PubMed] [Google Scholar]
  19. MATSANIOTIS N. S., TSENGHI C. J. MITOSIS IN LEUCOCYTE CULTURES. Lancet. 1964 May 2;1(7340):989–989. doi: 10.1016/s0140-6736(64)91797-0. [DOI] [PubMed] [Google Scholar]
  20. Mishell R. I., Dutton R. W. Immunization of dissociated spleen cell cultures from normal mice. J Exp Med. 1967 Sep 1;126(3):423–442. doi: 10.1084/jem.126.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Mitchell G. F., Miller J. F. Immunological activity of thymus and thoracic-duct lymphocytes. Proc Natl Acad Sci U S A. 1968 Jan;59(1):296–303. doi: 10.1073/pnas.59.1.296. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Mosier D. E. A requirement for two cell types for antibody formation in vitro. Science. 1967 Dec 22;158(3808):1573–1575. doi: 10.1126/science.158.3808.1573. [DOI] [PubMed] [Google Scholar]
  23. PEARMAIN G., LYCETTE R. R., FITZGERALD P. H. Tuberculin-induced mitosis in peripheral blood leucocytes. Lancet. 1963 Mar 23;1(7282):637–638. doi: 10.1016/s0140-6736(63)91275-3. [DOI] [PubMed] [Google Scholar]
  24. Parenti F., Franceschini P., Forti G., Cepellini R. The effect of phytohaemagglutinin on the metabolism and gamma-globulin synthesis of human lymphocytes. Biochim Biophys Acta. 1966 Jul 20;123(1):181–187. doi: 10.1016/0005-2787(66)90171-7. [DOI] [PubMed] [Google Scholar]
  25. SELL S., GELL P. G. STUDIES ON RABBIT LYMPHOCYTES IN VITRO. I. STIMULATION OF BLAST TRANSFORMATION WITH AN ANTIALLOTYPE SERUM. J Exp Med. 1965 Aug 1;122:423–440. doi: 10.1084/jem.122.2.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Sell S., Rowe D. S., Gell P. G. Studies on rabbit lymphocytes in vitro. 3. Proteins, RNA, and DNA synthesis by lymphocyte cultures after stimulation with phytohaemagglutinin, with staphylococcal filtrate, with antiallotype serum, and with heterologous antiserum to rabbit whole serum. J Exp Med. 1965 Oct 1;122(4):823–839. doi: 10.1084/jem.122.4.823. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Turner K. J., Forbes I. J. Synthesis of proteins by human leukocytes in vitro. II. Chemical characterization. J Immunol. 1966 Jun;96(6):926–935. [PubMed] [Google Scholar]
  28. van Furth R., Schuit H. R., Hijmans W. The formation of immunoglobulins by human tissues in vitro. IV. Circulating lymphocytes in normal and pathological conditions. Immunology. 1966 Jul;11(1):29–40. [PMC free article] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES