Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1969 May;4(5):555–578.

Monoclonal and polyclonal γM rheumatoid factors with anti-di- and anti-trinitrophenyl activity

K Hannestad
PMCID: PMC1579065  PMID: 5797417

Abstract

One M-component is described with anti-γG and anti-dinitrophenyl and anti-trinitrophenyl activity. The anti-hapten activity was shown by precipitation of DNP– and TNP–protein conjugates; this precipitation was specifically inhibited by the DNP–hapten. All of the M-component protein had these anti-hapten activities. The whole M-component also precipitated aggregated γG. DNP inhibited the anti-γG activity in precipitation and agglutination systems. Rheumatoid factors from sera of patients with classical rheumatoid arthritis exhibited similar, although weaker, reactivity with the haptens. The data indicate that rheumatoid factors cross-react with dinitrophenyl and trinitrophenyl groups.

Full text

PDF
555

Images in this article

561FIG. 1
on p.561
564FIG. 4
on p.564
567FIG. 8
on p.567

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BENACERRAF B., LEVINE B. B. Immunological specificity of delayed and immediate hypersensitivity reactions. J Exp Med. 1962 May 1;115:1023–1036. doi: 10.1084/jem.115.5.1023. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Balázs V., Fröhlich M. M. Anti-heavy chain activity of the monoclonal (paraprotein) cryoglobulins with rheumatoid factor effect. Am J Med Sci. 1966 Dec;252(6):668–674. [PubMed] [Google Scholar]
  3. Curtain C. C., Baumgarten A., Pye J. Coprecipitation of some cryomacroglobulins with immunoglobulins and their fragments. Arch Biochem Biophys. 1965 Oct;112(1):37–44. doi: 10.1016/0003-9861(65)90007-x. [DOI] [PubMed] [Google Scholar]
  4. EDELMAN G. M., KUNKEL H. G., FRANKLIN E. C. Interaction of the rheumatoid factor with antigen-antibody complexes and aggregated gamma globulin. J Exp Med. 1958 Jul 1;108(1):105–120. doi: 10.1084/jem.108.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. EISEN H. N., CARSTEN M. E., BELMAN S. Studies of hypersensitivity to low molecular weight substances. III. The 2,4-dinitrophenyl group as a determinant in the preciptin reaction. J Immunol. 1954 Nov;73(5):296–308. [PubMed] [Google Scholar]
  6. EISEN H. N. PREPARATION OF PURIFIED ANTI-2,4-DINITROPHENYL ANTIBODIES. Methods Med Res. 1964;10:94–102. [PubMed] [Google Scholar]
  7. FARAH F. S., KERN M., EISEN H. N. The preparation and some properties of purified antibody specific for the 2,4-dinitrophenyl group. J Exp Med. 1960 Dec 1;112:1195–1210. doi: 10.1084/jem.112.6.1195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. FRANKLIN E. C., HOLMAN H. R., MULLER-EBERHARD H. J., KUNKEL H. G. An unusual protein component of high molecular weight in the serum of certain patients with rheumatoid arthritis. J Exp Med. 1957 May 1;105(5):425–438. doi: 10.1084/jem.105.5.425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. FUDENBERG H. H., KUNKEL H. G. Specificity of the reaction between rheumatoid factors and gamma globulin. J Exp Med. 1961 Aug 1;114:257–278. doi: 10.1084/jem.114.2.257. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Forsgren A., Sjöquist J. "Protein A" from S. aureus. I. Pseudo-immune reaction with human gamma-globulin. J Immunol. 1966 Dec;97(6):822–827. [PubMed] [Google Scholar]
  11. GOODMAN J. W. Reaction of rheumatoid sera with fragments of papain-digested rabbit gamma globulin. Proc Soc Exp Biol Med. 1961 Apr;106:822–825. doi: 10.3181/00379727-106-26488. [DOI] [PubMed] [Google Scholar]
  12. GROSSBERG A., PRESSMAN D. PURIFICATION OF ANTI-P-AZOBENZOATE ANTIBODIES. Methods Med Res. 1964;10:103–105. [PubMed] [Google Scholar]
  13. Hanauer L. B., Christian C. L. Studies of cryoproteins in systemic lupus erythematosus. J Clin Invest. 1967 Mar;46(3):400–408. doi: 10.1172/JCI105541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hannestad K., Mellbye O. J. Rheumatoid factor in synovial effusions: local production and consumption. Clin Exp Immunol. 1967 Jul;2(4):501–509. [PMC free article] [PubMed] [Google Scholar]
  15. Hannestad K. Presence of aggregated gamma-G-globulin in certain rheumatoid synovial effusions. Clin Exp Immunol. 1967 Jul;2(4):511–529. [PMC free article] [PubMed] [Google Scholar]
  16. Hannestad K. Rheumatoid factors reacting with autologous native gamma-G-globulin and joint fluid gamma-G aggregates. Clin Exp Immunol. 1968 Sep;3(7):671–690. [PMC free article] [PubMed] [Google Scholar]
  17. KRITZMAN J., KUNKEL H. G., MCCARTHY J., MELLORS R. C. Studies of a Waldenstrom-type macroglobulin with rheumatoid factor properties. J Lab Clin Med. 1961 Jun;57:905–917. [PubMed] [Google Scholar]
  18. Klein F., van Rood J. J., van Furth R., Radema H. IgM-IgG cryoglobulinaemia with IgM paraprotein component. Clin Exp Immunol. 1968 Sep;3(7):703–716. [PMC free article] [PubMed] [Google Scholar]
  19. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  20. Lakin J. D., Patterson R., Pruzansky J. J. Immunoglobulins of the rhesus monkey (Macaca mulatta). I. Antiserum to bovine serum albumin and the dinitrophenyl antigenic determinant. J Immunol. 1967 Apr;98(4):745–756. [PubMed] [Google Scholar]
  21. Little J. R., Eisen H. N. Preparation and characterization of antibodies specific for the 2,4,6-trinitrophenyl group. Biochemistry. 1966 Nov;5(11):3385–3395. doi: 10.1021/bi00875a001. [DOI] [PubMed] [Google Scholar]
  22. MILGROM F., WITEBSKY E., GOLDSTEIN R., LOZA U. Studies on the rheumatoid and related serum factors. II. Relation of anti-human and anti-rabbit gamma globulin factors in rheumatoid arthritis serums. JAMA. 1962 Aug 11;181:476–484. [PubMed] [Google Scholar]
  23. Meltzer M., Franklin E. C., Elias K., McCluskey R. T., Cooper N. Cryoglobulinemia--a clinical and laboratory study. II. Cryoglobulins with rheumatoid factor activity. Am J Med. 1966 Jun;40(6):837–856. doi: 10.1016/0002-9343(66)90200-2. [DOI] [PubMed] [Google Scholar]
  24. Metzger H. Characterization of a human macroglobulin. V. A Waldenström macroglobulin with antibody activity. Proc Natl Acad Sci U S A. 1967 May;57(5):1490–1497. doi: 10.1073/pnas.57.5.1490. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Morse J. H. The aggregation of gamma-myeloma proteins. J Immunol. 1965 Oct;95(4):722–729. [PubMed] [Google Scholar]
  26. NISONOFF A., WISSLER F. C., LIPMAN L. N., WOERNLEY D. L. Separation of univalent fragments from the bivalent rabbit antibody molecule by reduction of disulfide bonds. Arch Biochem Biophys. 1960 Aug;89:230–244. doi: 10.1016/0003-9861(60)90049-7. [DOI] [PubMed] [Google Scholar]
  28. PORTER R. R. The hydrolysis of rabbit y-globulin and antibodies with crystalline papain. Biochem J. 1959 Sep;73:119–126. doi: 10.1042/bj0730119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Paul W. E., Benacerraf B. Problems encountered in double diffusion analysis in agar of hapten specific immune systems. II. The effect of the relative solubility of the immune complexes formed by cross-reacting systems. J Immunol. 1965 Dec;95(6):1074–1079. [PubMed] [Google Scholar]
  30. Paul W., Benacerraf B. Problems encountered in double diffusion analysis in agar of hapten specific immune systems. I. Complement dependent precipitation. J Immunol. 1965 Dec;95(6):1067–1073. [PubMed] [Google Scholar]
  31. SCHICK A. F., SINGER S. J. On the formation of covalent linkages between two protein molecules. J Biol Chem. 1961 Sep;236:2477–2485. [PubMed] [Google Scholar]
  32. SISKIND G. W. QUANTITATIVE STUDIES ON THE PRECIPITATION OF ANTI-2,4DINITROPHENYL ANTIBODY IN ANTIGEN EXCESS. J Immunol. 1964 May;92:702–708. [PubMed] [Google Scholar]
  33. WADSWORTH C., HANSON L. A. Comparative analysis of immune electrophoretic precipitates employing a modified immune electrophoretic technique. Int Arch Allergy Appl Immunol. 1960;17:165–177. doi: 10.1159/000229122. [DOI] [PubMed] [Google Scholar]
  34. WILLIAMS R. C., Jr, KUNKEL H. G. SEPARATION OF RHEUMATOID FACTORS OF DIFFERENT SPECIFICITIES USING COLUMNS CONJUGATED WITH GAMMA-GLOBULIN. Arthritis Rheum. 1963 Dec;6:665–675. doi: 10.1002/art.1780060602. [DOI] [PubMed] [Google Scholar]
  35. Zinneman H. H., Levi D., Seal U. S. On the nature of cryoglobulins. J Immunol. 1968 Mar;100(3):594–603. [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES