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. 1969 May;4(5):527–536.

Studies on DNA antibodies using DNA labelled with actinomycin-D (3H) or dimethyl (3H) sulphate

R I Carr, D Koffler, V Agnello, H G Kunkel
PMCID: PMC1579078  PMID: 5787344

Abstract

Native calf thymus DNA was labelled in vitro with tritiated actinomycin-D or tritiated dimethyl sulphate. These chemical methods yield DNA of high specific activity, without degradation or detectable alteration in reactivity with antibody to nucleic acid. The labelling procedures are simple, rapid and capable of producing relatively large amounts of radioactive DNA. Furthermore, they are applicable to a wide range of molecules of interest in the study of nucleic acid antibodies.

The labelled DNA was used in the ammonium sulphate precipitation technique to demonstrate DNA antibodies in the sera of patients with systemic lupus erythematosus. Whereas only nineteen of thirty-seven sera studied had demonstrable precipitins, thirty-three had significant binding ability for native DNA with this more sensitive assay. Serial studies on sera from a patient with systemic lupus erythematosus illustrate the value of this assay system for following the course of the disease.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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