Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1969 Aug;5(2):117–127.

On the mechanism of `rosette' formation in human and experimental thyroiditis

A Perrudet-Badoux, P C Frei
PMCID: PMC1579094  PMID: 5370052

Abstract

A method of immunocyto-adherence using circulating lymphocytes and sheep red cells coated with an antigen (rosette-formation) was studied in an attempt to detect lymphocytic antibodies. Human and experimental immune thyroiditis were chosen as a model. Such lymphocytic antibodies correlate better with the presence of immune thyroiditis than do humoral antibodies.

The principal aim of the present study was to try to explain the significance of the rosette-forming lymphocyte. The different experiments carried out for instance with the addition of an immunosuppressive drug or trypsin in vitro suggest that this lymphocyte may be representative of delayed type hypersensitivity. Other experiments allowed us to think that this cell was unable to passively fix circulating antibodies.

Full text

PDF
117

Images in this article

124FIG. 1
on p.124

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ahuja M. M., Tandon H. D., Jayaram B. M. Correlation of thyroid auto-antibodies to histological alterations in thyroid disease. J Assoc Physicians India. 1966 Aug;14(8):469–473. [PubMed] [Google Scholar]
  2. Anderson J. W., McConahey W. M., Alarcón-Segovia D., Emslander R. F., Wakim K. G. Diagnostic value of thyroid antibodies. J Clin Endocrinol Metab. 1967 Jul;27(7):937–944. doi: 10.1210/jcem-27-7-937. [DOI] [PubMed] [Google Scholar]
  3. Biozzi G., Stiffel C., Mouton D., Bouthillier Y., Decreusefond C. A kinetic study of antibody producing cells in the spleen of mice immunized intravenously with sheep erythrocytes. Immunology. 1968 Jan;14(1):7–20. [PMC free article] [PubMed] [Google Scholar]
  4. Biozzi G., Stiffel C., Mouton D., Bouthillier Y., Decreusefond C. Etude des cellules productrices d'anticorps par la méthode de l'immuno-cyto-adhérence. Pathol Biol. 1967 Apr;15(7):402–409. [PubMed] [Google Scholar]
  5. COULSON A. S., CHALMERS D. G. SEPARATION OF VIABLE LYMPHOCYTES FROM HUMAN BLOOD. Lancet. 1964 Feb 29;1(7331):468–469. doi: 10.1016/s0140-6736(64)90799-8. [DOI] [PubMed] [Google Scholar]
  6. Frei P. C., Cruchaud S. Recherche d'anticorps spécifiques dans les lymphocytes circulants au cours de diverses allergies chez l'homme. Helv Med Acta Suppl. 1966 May 15;46:161–162. [PubMed] [Google Scholar]
  7. Frei P. C., Cruchaud S., Vannotti A. Allergie à l'insuline de type cellulaire. Rev Fr Etud Clin Biol. 1965 Dec;10(10):1083–1087. [PubMed] [Google Scholar]
  8. HACKETT E., BEECH M., FORBES I. J. Thyrogloubulin antibodies in patients without clinical disease of the thyroid gland. Lancet. 1960 Aug 20;2(7147):402–404. doi: 10.1016/s0140-6736(60)92842-7. [DOI] [PubMed] [Google Scholar]
  9. HILL O. W. Thyroglobulin antibodies in 1,297 patients without thyroid disease. Br Med J. 1961 Jun 24;1(5242):1793–1796. doi: 10.1136/bmj.1.5242.1793. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. HULLIGER L., SORKIN E. Synthesis of antibodies by blood leucocytes of the rabbit. Nature. 1963 Apr 20;198:299–299. doi: 10.1038/198299a0. [DOI] [PubMed] [Google Scholar]
  11. LANDY M., SANDERSON R. P., BERNSTEIN M. T., JACKSON A. L. ANTIBODY PRODUCTION BY LEUCOCYTES IN PERIPHERAL BLOOD. Nature. 1964 Dec 26;204:1320–1321. doi: 10.1038/2041320a0. [DOI] [PubMed] [Google Scholar]
  12. NOTA N. R., LIACOPOULOS-BRIOT M., STIFFEL C., BIOZZI G. L'IMMUNO-CYTO-ADH'ERENCE: UNE M'ETHODE SIMPLE ET QUANTITATIVE POUR L'ETUDE IN VITRO DES CELLULES PRODUCTRICES D'ANTICORPS. C R Hebd Seances Acad Sci. 1964 Aug 3;259:1277–1280. [PubMed] [Google Scholar]
  13. ROITT I. M., DONIACH D. Human autoimmune thyroiditis: serological studies. Lancet. 1958 Nov 15;2(7055):1027–1033. doi: 10.1016/s0140-6736(58)90530-0. [DOI] [PubMed] [Google Scholar]
  14. UI N., TARUTANI O., KONDO Y., TAMURA H. Chromatographic fractionation of hog thyroglobulin. Nature. 1961 Sep 16;191:1199–1201. doi: 10.1038/1911199a0. [DOI] [PubMed] [Google Scholar]
  15. WITEBSKY E., ROSE N. R. Studies on organ specificity. VII. Production of antibodies to rabbit thyroid by injection of foreign thyroid extracts. J Immunol. 1959 Jul;83(1):41–48. [PubMed] [Google Scholar]
  16. ZAALBERG O. B. A SIMPLE METHOD FOR DETECTING SINGLE ANTIBODY-FORMING CELLS. Nature. 1964 Jun 20;202:1231–1231. doi: 10.1038/2021231a0. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES