Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1969 Nov;5(5):515–524.

Persistence of thyroid microsomal and thyroglobulin antigens in primary cultures of human thyroid epithelium

T Ghose, Mildred Cerini
PMCID: PMC1579136  PMID: 4902605

Abstract

The changes in the amount and distribution of thyroglobulin and thyroid `microsomal' antigens were studied in primary explant and monolayer cultures of human thyroid epithelium which were alive for about 60 days. Both explant and monolayer cultures produced thyroglobulin in vitro at all times during the 60-day period of observation. However, the number of cells containing the thyroid `microsomal' antigen and the amount of antigen in individual cells both diminished though the antigen was detectable in a proportion of viable cells in all cultures at all times during the 60-day period of observation of this report.

Full text

PDF
515

Images in this article

518FIG. 1
on p.518
519FIG. 2
on p.519

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cahn R. D., Cahn M. B. Heritability of cellular differentiation: clonal growth and expression of differentiation in retinal pigment cells in vitro. Proc Natl Acad Sci U S A. 1966 Jan;55(1):106–114. doi: 10.1073/pnas.55.1.106. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cahn R. D., Lasher R. Simultaneous synthesis of DNA and specialized cellular products by differentiating cartilage cells in vitro. Proc Natl Acad Sci U S A. 1967 Sep;58(3):1131–1138. doi: 10.1073/pnas.58.3.1131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Coon H. G., Cahn R. D. Differentiation in vitro: effects of Sephadex fractions of chick embryo extract. Science. 1966 Sep 2;153(3740):1116–1119. doi: 10.1126/science.153.3740.1116. [DOI] [PubMed] [Google Scholar]
  4. Coon H. G. Clonal stability and phenotypic expression of chick cartilage cells in vitro. Proc Natl Acad Sci U S A. 1966 Jan;55(1):66–73. doi: 10.1073/pnas.55.1.66. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Davies L. M., Priest J. H., Priest R. E. Collagen synthesis by cells synchronously replicating DNA. Science. 1968 Jan 5;159(3810):91–93. [PubMed] [Google Scholar]
  6. FORBES I. J., ROITT I. M., DONIACH D., SOLOMON I. L. The thyroid cytotoxic autoantibody. J Clin Invest. 1962 May;41:996–1006. doi: 10.1172/JCI104579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. GOUDIE R. B., MCCALLUM H. M. LOSS OF TISSUE-SPECIFIC AUTOANTIGEN IN THYROID TUMOURS: A DEMONSTRATION BY IMMUNOFLUORESCENCE. Lancet. 1963 Nov 16;2(7316):1035–1038. doi: 10.1016/s0140-6736(63)90008-4. [DOI] [PubMed] [Google Scholar]
  8. GOUDIE R. B., McCALLUM H. M. Loss of tissue-specific autoantigens in thyroid tumours. Lancet. 1962 Feb 17;1(7225):348–352. doi: 10.1016/s0140-6736(62)91302-8. [DOI] [PubMed] [Google Scholar]
  9. Ghose T., Nairn R. C., Cerini M. Persistence of gastrointestinal-specific antigens in primary culture of human colon epithelium. Exp Cell Res. 1968 Mar;49(3):513–519. doi: 10.1016/0014-4827(68)90201-2. [DOI] [PubMed] [Google Scholar]
  10. Irvine W. J. Studies on the Cytotoxic Factor in Thyroid Disease. Br Med J. 1962 May 26;1(5290):1444–1442.4. doi: 10.1136/bmj.1.5290.1444. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kuhns W. J., Bramson S. Variable behaviour of blood group H on HeLa cell populations synchronized with thymidine. Nature. 1968 Aug 31;219(5157):938–939. doi: 10.1038/219938a0. [DOI] [PubMed] [Google Scholar]
  12. Nicol A. G., Beck J. S. Persistence of an organ-specific antigen in organ and tissue cultures of hyperplastic human thyroid gland. Nature. 1966 Jun 18;210(5042):1227–1229. doi: 10.1038/2101227a0. [DOI] [PubMed] [Google Scholar]
  13. Okada T. S. Changes in antigenic constitutions of embryonic chicken kidney cells during in vitro spreading culture. Exp Cell Res. 1965 Sep;39(2):591–603. doi: 10.1016/0014-4827(65)90061-3. [DOI] [PubMed] [Google Scholar]
  14. PULVERTAFT R. J., DONIACH D., ROITT I. M. The cytotoxic factor in Hashimoto's disease and its incidence in other thyroid diseases. Br J Exp Pathol. 1961 Oct;42:496–503. [PMC free article] [PubMed] [Google Scholar]
  15. ROITT I. M., LING N. R., DONIACH D., COUCHMAN K. G. THE CYTOPLASMIC AUTO-ANTIGEN OF THE HUMAN THYROID. I. IMMUNOLOGICAL AND BIOCHEMICAL CHARACTERISTICS. Immunology. 1964 Jul;7:375–393. [PMC free article] [PubMed] [Google Scholar]
  16. Rubin H. A substance in conditioned medium which enhances the growth of small numbers of chick embryo cells. Exp Cell Res. 1966 Jan;41(1):138–148. doi: 10.1016/0014-4827(66)90554-4. [DOI] [PubMed] [Google Scholar]
  17. van Furth R., Schuit R. E., Hijmans W. The formation of immunoglobulins by human tissues in vitro. I. The methods and their specificity. Immunology. 1966 Jul;11(1):1–11. [PMC free article] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES