Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1966 Jan;1(1):45–60.

In vitro cytotoxic effects of lymphoid cells from rats with experimental autoimmune nephrosis

G Holm
PMCID: PMC1579167  PMID: 4958208

Abstract

A nephrotic syndrome was induced in randomly bred Sprague-Dawley rats by repeated intraperitoneal injections of homologous kidney extract and Freund's complete adjuvant. Cell injury in vitro was measured by estimating the release of radioactivity from damaged target cells labelled with 14C-thymidine. Various conditions influencing the reproducibility of the isotope method were investigated.

Blood lymphoid cells, lymph node cells and spleen cells from these rats damaged primary monolayer cultures of rat kidney cells after 18 hr incubation. Lymphoid cells from liver sensitized rats were also cytotoxic to rat kidney cells, whereas lymphoid cells from rats injected with other rat tissue extracts (lung or spleen) or given only adjuvant did not react. Primary cultures of rat lung cells were sensitive while cells of a rat liver cell strain were resistant to the cytotoxic action of lymphoid cells from kidney sensitized rats. Sera from nephrotic rats did not damage rat kidney cells under the experimental conditions used here.

Full text

PDF
45

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BACH F. H., HIRSCHHORN K. THE IN VITRO IMMUNE RESPONSE OF PERIPHERAL BLOOD LYMPHOCYTES. Semin Hematol. 1965 Jan;2:68–89. [PubMed] [Google Scholar]
  2. BERG O., KAELLEN B. EFFECT OF MONONUCLEAR BLOOD CELLS FROM MULTIPLE SCLEROSIS PATIENTS ON NEUROGLIA IN TISSUE CULTURE. J Neuropathol Exp Neurol. 1964 Jul;23:550–559. doi: 10.1097/00005072-196407000-00010. [DOI] [PubMed] [Google Scholar]
  3. BIOERKLUND A. TESTING IN VITRO OF LYMPHOID CELLS FROM RATS WITH EXPERIMENTAL AUTOIMMUNE THYROIDITIS. Lab Invest. 1964 Feb;13:120–127. [PubMed] [Google Scholar]
  4. BRAND K. G., SYVERTON J. T. Results of species-specific hemagglutination tests on "transformed," nontransformed, and primary cell cultures. J Natl Cancer Inst. 1962 Jan;28:147–157. [PubMed] [Google Scholar]
  5. GREEN H., FLEISCHER R. A., BARROW P., GOLDBERG B. The cytotoxic action of immune gamma globulin and complement on Krebs ascites tumor cells. II. Chemical studies. J Exp Med. 1959 May 1;109(5):511–521. doi: 10.1084/jem.109.5.511. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HESS E. V., ASHWORTH C. T., ZIFF M. Transfer of an autoimmune nephrosis in the rat by means of lymph node cells. J Exp Med. 1962 Feb 1;115:421–438. doi: 10.1084/jem.115.2.421. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HEYMANN W., HACKEL D. B., HARWOOD S., WILSON S. G., HUNTER J. L. Production of nephrotic syndrome in rats by Freund's adjuvants and rat kidney suspensions. Proc Soc Exp Biol Med. 1959 Apr;100(4):660–664. doi: 10.3181/00379727-100-24736. [DOI] [PubMed] [Google Scholar]
  8. HEYMANN W., HUNTER J. L., HACKEL D. B., CUPPAGE F. Transfer of experimental auto-immune nephrosis in rats. Proc Soc Exp Biol Med. 1962 Dec;111:568–573. doi: 10.3181/00379727-111-27857. [DOI] [PubMed] [Google Scholar]
  9. HIRATA A. A. CYTOLYTIC ANTIBODY ASSAY BY TRYPTIC DIGESTION OF INJURED CELLS AND ELECTRONIC COUNTING. J Immunol. 1963 Nov;91:625–632. [PubMed] [Google Scholar]
  10. HOLMBERG B. On the permeability to lissamine green and other dyes in the course of cell injury and cell death. Exp Cell Res. 1961 Jan;22:406–414. doi: 10.1016/0014-4827(61)90118-5. [DOI] [PubMed] [Google Scholar]
  11. HOLM G., PERLMANN P., WERNER B. PHYTOHAEMAGGLUTININ-INDUCED CYTOTOXIC ACTION OF NORMAL LYMPHOID CELLS ON CELLS IN TISSUE CULTURE. Nature. 1964 Aug 22;203:841–843. doi: 10.1038/203841a0. [DOI] [PubMed] [Google Scholar]
  12. Holm G., Perlmann P. Phytohaemagglutinin-induced cytotoxic action of unsensitized immunologically competent cells on allogeneic and xenogeneic tissue culture cells. Nature. 1965 Aug 21;207(999):818–821. doi: 10.1038/207818a0. [DOI] [PubMed] [Google Scholar]
  13. KLEIN G., PERLMANN P. IN VITRO CYTOTOXIC EFFECT OF ISOANTIBODY MEASURED AS ISOTOPE RELEASE FROM LABELLED TARGET CELL DNA. Nature. 1963 Aug 3;199:451–453. doi: 10.1038/199451a0. [DOI] [PubMed] [Google Scholar]
  14. KOPROWSKI H., FERNANDES M. V. Autosensitization reaction in vitro. Contactual agglutination of sensitized lymph node cells in brain tissue culture accompanied by destruction of glial elements. J Exp Med. 1962 Oct 1;116:467–476. doi: 10.1084/jem.116.4.467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  16. MOELLER E. ANTAGONISTIC EFFECTS OF HUMORAL ISOANTIBODIES ON THE IN VITRO CYTOTOXICITY OF IMMUNE LYMPHOID CELLS. J Exp Med. 1965 Jul 1;122:11–23. doi: 10.1084/jem.122.1.11. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. MOELLER E. CONTACT-INDUCED CYTOTOXICITY BY LYMPHOID CELLS CONTAINING FOREIGN ISOANTIGENS. Science. 1965 Feb 19;147(3660):873–879. doi: 10.1126/science.147.3660.873. [DOI] [PubMed] [Google Scholar]
  18. MORGAN W. S., PERLMANN P., HULTIN T. Autoradiographic analysis on agar plates of antigens from subcellular fractions of rat liver slices. J Biophys Biochem Cytol. 1961 Jul;10:411–423. doi: 10.1083/jcb.10.3.411. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. OUCHTERLONY O. Diffusion-in-gel methods for immunological analysis. Prog Allergy. 1958;5:1–78. [PubMed] [Google Scholar]
  20. PATERSON P. Y. Transfer of allergic encephalomyelitis in rats by means of lymph node cells. J Exp Med. 1960 Jan 1;111:119–136. doi: 10.1084/jem.111.1.119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. PERLMANN P., BROBERGER O. In vitro studies of ulcerative colitis. II. Cytotoxic action of white blood cells from patients on human fetal colon cells. J Exp Med. 1963 May 1;117:717–733. doi: 10.1084/jem.117.5.717. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. POKORNA Z., VOJTISKOVA M. AUTOIMMUNE DAMAGE OF THE TESTES INDUCED WITH CHEMICALLY MODIFIED ORGAN SPECIFIC ANTIGEN. Folia Biol (Praha) 1964;10:261–267. [PubMed] [Google Scholar]
  23. ROSENAU W., MOON H. D. Lysis of homologous cells by sensitized lymphocytes in tissue culture. J Natl Cancer Inst. 1961 Aug;27:471–483. [PubMed] [Google Scholar]
  24. ROSENAU W., MOON H. D. THE SPECIFICITY OF THE CYTOLYTIC EFFECT OF SENSITIZED LYMPHOID CELLS IN VITRO. J Immunol. 1964 Dec;93:910–914. [PubMed] [Google Scholar]
  25. SCHREK R. Qualitative and quantitative reactions of lymphocytes to x rays. Ann N Y Acad Sci. 1961 Nov 13;95:839–848. doi: 10.1111/j.1749-6632.1961.tb50080.x. [DOI] [PubMed] [Google Scholar]
  26. STAVITSKY A. B. Micromethods for the study of proteins and antibodies. I. Procedure and general applications of hemagglutination and hemagglutination-inhibition reactions with tannic acid and protein-treated red blood cells. J Immunol. 1954 May;72(5):360–367. [PubMed] [Google Scholar]
  27. TROWELL O. A. The culture of mature organs in a synthetic medium. Exp Cell Res. 1959 Jan;16(1):118–147. doi: 10.1016/0014-4827(59)90201-0. [DOI] [PubMed] [Google Scholar]
  28. VAINIO T., KOSKIMIES O., PERLMANN P., PERLMANN H., KLEIN G. IN VITRO CYTOTOXIC EFFECT OF LYMPHOID CELLS FROM MICE IMMUNIZED WITH ALLOGENEIC TISSUE. Nature. 1964 Oct 31;204:453–455. doi: 10.1038/204453a0. [DOI] [PubMed] [Google Scholar]
  29. WEIGLE W. O. THE INDUCTION OF AUTOIMMUNITY IN RABBITS FOLLOWING INJECTION OF HETEROLOGOUS OR ALTERED HOMOLOGOUS THYROGLOBULIN. J Exp Med. 1965 Feb 1;121:289–308. doi: 10.1084/jem.121.2.289. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES