Abstract
An automated technique has been established for the screening and titration of sera for complement-fixing antibody to gastric (parietal) cell and thyroid microsomal antigens. The automated method is of equal sensitivity to the manual complement-fixation technique but slightly less sensitive compared to the indirect fluorescent antibody method. The automated method has the advantage over the manual complement-fixation test of greater accuracy and reproducibility and the element of subjective interpretation is removed. It is less demanding on technician skill. The automated method requires only small amounts of sera but it consumes a considerable amount of antigen. Human antigen has been used in the present study.
The automated method for complement-fixation reactions has some advantages over the manual method for both routine clinical purposes and for the research laboratory.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BUCHANAN W. W., KOUTRAS D. A., CROOKS J., ALEXANDER W. D., BRASS W., ANDERSON J. R., GOUDIE R. B., GRAY K. G. The clinical significance of the complement fixation test in thyrotoxicosis. J Endocrinol. 1962 Apr;24:115–125. doi: 10.1677/joe.0.0240115. [DOI] [PubMed] [Google Scholar]
- IRVINE W. J. GASTRIC ANTIBODIES STUDIED BY FLUORESCENCE MICROSCOPY. Q J Exp Physiol Cogn Med Sci. 1963 Oct;48:427–438. doi: 10.1113/expphysiol.1963.sp001685. [DOI] [PubMed] [Google Scholar]
- IRVINE W. J. IMMUNOLOGIC ASPECTS OF PERNICIOUS ANEMIA. N Engl J Med. 1965 Aug 19;273:432–438. doi: 10.1056/NEJM196508192730807. [DOI] [PubMed] [Google Scholar]
- Irvine W. J., Davies S. H., Teitelbaum S., Delamore I. W., Williams A. W. The clinical and pathological significance of gastric parietal cell antibody. Ann N Y Acad Sci. 1965 Jun 30;124(2):657–691. doi: 10.1111/j.1749-6632.1965.tb18993.x. [DOI] [PubMed] [Google Scholar]
- ROITT I. M., DONIACH D. Thyroid auto-immunity. Br Med Bull. 1960 May;16:152–158. doi: 10.1093/oxfordjournals.bmb.a069816. [DOI] [PubMed] [Google Scholar]
- TAYLOR K. B., ROITT I. M., DONIACH D., COUCHMAN K. G., SHAPLAND C. Autoimmune phenomena in pernicious anaemia: gastric antibodies. Br Med J. 1962 Nov 24;2(5316):1347–1352. doi: 10.1136/bmj.2.5316.1347. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TROTTER W. R., BELYAVIN G., WADDAMS A. Precipitating and complement-fixing antibodies in Hashimoto's disease. Proc R Soc Med. 1957 Nov;50(11):961–962. [PubMed] [Google Scholar]