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. 1996 Sep;112(1):53–59. doi: 10.1104/pp.112.1.53

Influence of Auxin and Gibberellin on in Vivo Protein Synthesis during Early Pea Fruit Growth.

R Van Huizen 1, J A Ozga 1, D M Reinecke 1
PMCID: PMC157922  PMID: 12226372

Abstract

Developing pea fruits (Pisum sativum L.) offer a unique opportunity to study growth and development in a tissue that is responsive to both gibberellins (GAs) and auxin (4-chloroindole-3-acetic acid[4-CI-IAA]). To begin a molecular analysis of the interaction of GAs and auxins in pea fruit development, in vivo labeling with [35S]methionine coupled with two-dimensional gel electrophoresis were used to characterize de novo synthesis of proteins during gibberellic acid (GA3)-, 4-CI-indoleacetic acid-, and seed-induced pea pericarp growth. The most significant and reproducible polypeptide changes were observed between molecular weights of 20 and 60. Comparing about 250 de novo synthesized proteins revealed that seed removal changed the pattern substantially. We identified one class of polypeptides that was uniquely seed induced and five classes that were affected by hormone treatment. The latter included 4-CI-IAA-induced, GA3-induced, GA3- and 4-CI-IAA-induced, 4-CI-IAA-repressed, and GA3- and 4-CI-IAA-repressed polypeptides. Similar patterns of protein expression were associated with both hormone treatments; however, changes unique to GA3 or 4-CI-IAA treatment also indicate that the effects of GA3 and 4-CI-IAA on this process are not equivalent. In general, application of 4-CI-IAA plus GA3 replaced the seed effects on pericarp protein synthesis, supporting our hypothesis that both hormones are involved in pea pericarp development.

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Selected References

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  1. Chory J., Voytas D. F., Olszewski N. E., Ausubel F. M. Gibberellin-Induced Changes in the Populations of Translatable mRNAs and Accumulated Polypeptides in Dwarfs of Maize and Pea. Plant Physiol. 1987 Jan;83(1):15–23. doi: 10.1104/pp.83.1.15. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Gillaspy G., Ben-David H., Gruissem W. Fruits: A Developmental Perspective. Plant Cell. 1993 Oct;5(10):1439–1451. doi: 10.1105/tpc.5.10.1439. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Granell A., Harris N., Pisabarro A. G., Carbonell J. Temporal and spatial expression of a thiolprotease gene during pea ovary senescence, and its regulation by gibberellin. Plant J. 1992 Nov;2(6):907–915. doi: 10.1046/j.1365-313x.1992.t01-5-00999.x. [DOI] [PubMed] [Google Scholar]
  4. Gray J., Picton S., Shabbeer J., Schuch W., Grierson D. Molecular biology of fruit ripening and its manipulation with antisense genes. Plant Mol Biol. 1992 May;19(1):69–87. doi: 10.1007/BF00015607. [DOI] [PubMed] [Google Scholar]
  5. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  6. Ledger S. E., Gardner R. C. Cloning and characterization of five cDNAs for genes differentially expressed during fruit development of kiwifruit (Actinidia deliciosa var. deliciosa). Plant Mol Biol. 1994 Aug;25(5):877–886. doi: 10.1007/BF00028882. [DOI] [PubMed] [Google Scholar]
  7. Marumo S., Hattori H., Abe H., Munakata K. Isolation of 4-chloroindolyl-3-acetic acid from immature seeds of Pisum sativum. Nature. 1968 Aug 31;219(5157):959–960. doi: 10.1038/219959b0. [DOI] [PubMed] [Google Scholar]
  8. Narita J. O., Gruissem W. Tomato hydroxymethylglutaryl-CoA reductase is required early in fruit development but not during ripening. Plant Cell. 1989 Feb;1(2):181–190. doi: 10.1105/tpc.1.2.181. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ozga J. A., Brenner M. L., Reinecke D. M. Seed effects on gibberellin metabolism in pea pericarp. Plant Physiol. 1992 Sep;100(1):88–94. doi: 10.1104/pp.100.1.88. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Reddy A. S., Jena P. K., Mukherjee S. K., Poovaiah B. W. Molecular cloning of cDNAs for auxin-induced mRNAs and developmental expression of the auxin-inducible genes. Plant Mol Biol. 1990 May;14(5):643–653. doi: 10.1007/BF00016498. [DOI] [PubMed] [Google Scholar]
  11. Reddy A. S., Poovaiah B. W. Molecular cloning and sequencing of a cDNA for an auxin-repressed mRNA: correlation between fruit growth and repression of the auxin-regulated gene. Plant Mol Biol. 1990 Feb;14(2):127–136. doi: 10.1007/BF00018554. [DOI] [PubMed] [Google Scholar]
  12. Salts Y., Wachs R., Gruissem W., Barg R. Sequence coding for a novel proline-rich protein preferentially expressed in young tomato fruit. Plant Mol Biol. 1991 Jul;17(1):149–150. doi: 10.1007/BF00036818. [DOI] [PubMed] [Google Scholar]
  13. Shi L., Gast R. T., Gopalraj M., Olszewski N. E. Characterization of a shoot-specific, GA3- and ABA-regulated gene from tomato. Plant J. 1992 Mar;2(2):153–159. [PubMed] [Google Scholar]
  14. Veluthambi K., Poovaiah B. W. Auxin-regulated polypeptide changes at different stages of strawberry fruit development. Plant Physiol. 1984 Jun;75(2):349–353. doi: 10.1104/pp.75.2.349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Xin Z., Li P. H. Alteration of Gene Expression Associated with Abscisic Acid-Induced Chilling Tolerance in Maize Suspension-Cultured Cells. Plant Physiol. 1993 Jan;101(1):277–284. doi: 10.1104/pp.101.1.277. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. van Huizen R., Ozga J. A., Reinecke D. M., Twitchin B., Mander L. N. Seed and 4-chloroindole-3-acetic acid regulation of gibberellin metabolism in pea pericarp. Plant Physiol. 1995 Dec;109(4):1213–1217. doi: 10.1104/pp.109.4.1213. [DOI] [PMC free article] [PubMed] [Google Scholar]

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