Abstract
Alleles of avirulence gene D (avrD) specify the production by bacteria of syringolides that elicit the hypersensitive response in soybean (Glycine max) plants carrying the disease-resistance gene Rpg4, but not rpg4 plants. Syringolide 1 caused extracellular alkalization, K+ efflux, and Ca2+ influx about 30 min after addition to suspension-cultured cells of two Rpg4 cultivars, Harosoy and Flambeau, but not in two rpg4 cultivars, Acme and Merit. All responses were sustained for at least 1.5 h and were inhibited by La3+, which blocks certain Ca2+ channels. These results suggest that syringolide 1 activates a Ca2+ influx-dependent signaling pathway only in Rpg4 soybean cells.
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Selected References
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- Atkinson M. M., Keppler L. D., Orlandi E. W., Baker C. J., Mischke C. F. Involvement of plasma membrane calcium influx in bacterial induction of the k/h and hypersensitive responses in tobacco. Plant Physiol. 1990 Jan;92(1):215–221. doi: 10.1104/pp.92.1.215. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Baker C. J., Orlandi E. W., Mock N. M. Harpin, An Elicitor of the Hypersensitive Response in Tobacco Caused by Erwinia amylovora, Elicits Active Oxygen Production in Suspension Cells. Plant Physiol. 1993 Aug;102(4):1341–1344. doi: 10.1104/pp.102.4.1341. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Culver J. N., Dawson W. O. Tobacco mosaic virus coat protein: an elicitor of the hypersensitive reaction but not required for the development of mosaic symptoms in Nicotiana sylvestris. Virology. 1989 Dec;173(2):755–758. doi: 10.1016/0042-6822(89)90592-8. [DOI] [PubMed] [Google Scholar]
- Gross P., Julius C., Schmelzer E., Hahlbrock K. Translocation of cytoplasm and nucleus to fungal penetration sites is associated with depolymerization of microtubules and defence gene activation in infected, cultured parsley cells. EMBO J. 1993 May;12(5):1735–1744. doi: 10.1002/j.1460-2075.1993.tb05821.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Keen N. T. Gene-for-gene complementarity in plant-pathogen interactions. Annu Rev Genet. 1990;24:447–463. doi: 10.1146/annurev.ge.24.120190.002311. [DOI] [PubMed] [Google Scholar]
- Levine A., Pennell R. I., Alvarez M. E., Palmer R., Lamb C. Calcium-mediated apoptosis in a plant hypersensitive disease resistance response. Curr Biol. 1996 Apr 1;6(4):427–437. doi: 10.1016/s0960-9822(02)00510-9. [DOI] [PubMed] [Google Scholar]
- Martin G. B., Brommonschenkel S. H., Chunwongse J., Frary A., Ganal M. W., Spivey R., Wu T., Earle E. D., Tanksley S. D. Map-based cloning of a protein kinase gene conferring disease resistance in tomato. Science. 1993 Nov 26;262(5138):1432–1436. doi: 10.1126/science.7902614. [DOI] [PubMed] [Google Scholar]
- Renard D. C., Bolton M. M., Rhee S. G., Margolis B. L., Zilberstein A., Schlessinger J., Thomas A. P. Modified kinetics of platelet-derived growth factor-induced Ca2+ increases in NIH-3T3 cells overexpressing phospholipase C gamma 1. Biochem J. 1992 Feb 1;281(Pt 3):775–784. doi: 10.1042/bj2810775. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Staskawicz B. J., Ausubel F. M., Baker B. J., Ellis J. G., Jones J. D. Molecular genetics of plant disease resistance. Science. 1995 May 5;268(5211):661–667. doi: 10.1126/science.7732374. [DOI] [PubMed] [Google Scholar]
- Van den Ackerveken G. F., Van Kan J. A., De Wit P. J. Molecular analysis of the avirulence gene avr9 of the fungal tomato pathogen Cladosporium fulvum fully supports the gene-for-gene hypothesis. Plant J. 1992 May;2(3):359–366. doi: 10.1111/j.1365-313x.1992.00359.x. [DOI] [PubMed] [Google Scholar]
- Yucel I., Midland S. L., Sims J. J., Keen N. T. Class I and class II avrD alleles direct the production of different products in gram-negative bacteria. Mol Plant Microbe Interact. 1994 Jan-Feb;7(1):148–150. doi: 10.1094/mpmi-7-0148. [DOI] [PubMed] [Google Scholar]