Abstract
We have altered the growth and development of a deciduous forest tree by transforming hybrid aspen (Populus tremula x Populus tremuloides) with the Agrobacterium rhizogenes rolC gene expressed under the strong cauliflower mosaic virus 35S promoter. We demonstrate that the genetically manipulated perennial plants, after a period of dormancy, maintain the induced phenotypical changes during the second growing period. Furthermore, mass-spectrometrical quantifications of the free and conjugated forms of indole-3-acetic acid and cytokinins and several gibberellins on one transgenic line correlate the induced developmental alterations such as stem fasciation to changes in plant hormone metabolism. We also show that the presence of the RolC protein increases the levels of the free cytokinins, but not by a process involving hydrolysis of the inactive cytokinin conjugates.
Full Text
The Full Text of this article is available as a PDF (2.1 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Clark S. E., Running M. P., Meyerowitz E. M. CLAVATA1, a regulator of meristem and flower development in Arabidopsis. Development. 1993 Oct;119(2):397–418. doi: 10.1242/dev.119.2.397. [DOI] [PubMed] [Google Scholar]
- Crespi M., Messens E., Caplan A. B., van Montagu M., Desomer J. Fasciation induction by the phytopathogen Rhodococcus fascians depends upon a linear plasmid encoding a cytokinin synthase gene. EMBO J. 1992 Mar;11(3):795–804. doi: 10.1002/j.1460-2075.1992.tb05116.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Croker S. J., Hedden P., Lenton J. R., Stoddart J. L. Comparison of gibberellins in normal and slender barley seedlings. Plant Physiol. 1990 Sep;94(1):194–200. doi: 10.1104/pp.94.1.194. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Estruch J. J., Chriqui D., Grossmann K., Schell J., Spena A. The plant oncogene rolC is responsible for the release of cytokinins from glucoside conjugates. EMBO J. 1991 Oct;10(10):2889–2895. doi: 10.1002/j.1460-2075.1991.tb07838.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Medford J. I., Behringer F. J., Callos J. D., Feldmann K. A. Normal and Abnormal Development in the Arabidopsis Vegetative Shoot Apex. Plant Cell. 1992 Jun;4(6):631–643. doi: 10.1105/tpc.4.6.631. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nilsson O., Moritz T., Imbault N., Sandberg G., Olsson O. Hormonal Characterization of Transgenic Tobacco Plants Expressing the rolC Gene of Agrobacterium rhizogenes TL-DNA. Plant Physiol. 1993 Jun;102(2):363–371. doi: 10.1104/pp.102.2.363. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schmülling T., Schell J., Spena A. Single genes from Agrobacterium rhizogenes influence plant development. EMBO J. 1988 Sep;7(9):2621–2629. doi: 10.1002/j.1460-2075.1988.tb03114.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Talon M., Zeevaart J. A. Gibberellins and Stem Growth as Related to Photoperiod in Silene armeria L. Plant Physiol. 1990 Apr;92(4):1094–1100. doi: 10.1104/pp.92.4.1094. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Weigel D., Nilsson O. A developmental switch sufficient for flower initiation in diverse plants. Nature. 1995 Oct 12;377(6549):495–500. doi: 10.1038/377495a0. [DOI] [PubMed] [Google Scholar]
- White F. F., Taylor B. H., Huffman G. A., Gordon M. P., Nester E. W. Molecular and genetic analysis of the transferred DNA regions of the root-inducing plasmid of Agrobacterium rhizogenes. J Bacteriol. 1985 Oct;164(1):33–44. doi: 10.1128/jb.164.1.33-44.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]