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. 1996 Oct;112(2):821–832. doi: 10.1104/pp.112.2.821

Severity of mutant phenotype in a series of chlorophyll-deficient wheat mutants depends on light intensity and the severity of the block in chlorophyll synthesis.

T G Falbel 1, J B Meehl 1, L A Staehelin 1
PMCID: PMC158007  PMID: 8883392

Abstract

Analyses of a series of allelic chlorina mutants of wheat (Triticum aestivum L.), which have partial blocks in chlorophyll (Chl) synthesis and, therefore, a limited Chl supply, reinforce the principle that Chl is required for the stable accumulation of Chl-binding proteins and that only reaction centers accumulate when the supply of Chl is severely limited. Depending on the rate of Chl accumulation (determined by the severity of the mutation) and on the rate of turnover of Chl and its precursors (determined by the environment in which the plant is grown), the mutants each reach an equilibrium of Chl synthesis and degradation. Together these mutants generate a spectrum of phenotypes. Under the harshest conditions (high illumination), plants with moderate blocks in Chl synthesis have membranes with very little Chl and Chl-proteins and membrane stacks resembling the thylakoids of the lethal xantha mutants of barely grown at low to medium light intensities (which have more severe blocks). In contrast, when grown under low-light conditions the same plants with moderate blocks have thylakoids resembling those of the wild type. The wide range of phenotypes of Chl b-deficient mutants has historically produced more confusion than enlightenment, but incomparable growth conditions can now explain the discrepancies reported in the literature.

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Selected References

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  1. Akoyunoglou A., Akoyunoglou G. Reorganization of Thylakoid Components during Chloroplast Development in Higher Plants after Transfer to Darkness : Changes in Photosystem I Unit Components, and in Cytochromes. Plant Physiol. 1985 Oct;79(2):425–431. doi: 10.1104/pp.79.2.425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bassi R., Simpson D. Chlorophyll-protein complexes of barley photosystem I. Eur J Biochem. 1987 Mar 2;163(2):221–230. doi: 10.1111/j.1432-1033.1987.tb10791.x. [DOI] [PubMed] [Google Scholar]
  3. Bellemare G., Bartlett S. G., Chua N. H. Biosynthesis of chlorophyll a/b-binding polypeptides in wild type and the chlorina f2 mutant of barley. J Biol Chem. 1982 Jul 10;257(13):7762–7767. [PubMed] [Google Scholar]
  4. Bennett J. Biosynthesis of the light-harvesting chlorophyll a/b protein. Polypeptide turnover in darkness. Eur J Biochem. 1981 Aug;118(1):61–70. doi: 10.1111/j.1432-1033.1981.tb05486.x. [DOI] [PubMed] [Google Scholar]
  5. De Greef J., Butler W. L., Roth T. F. Greening of etiolated bean leaves in far red light. Plant Physiol. 1971 Apr;47(4):457–464. doi: 10.1104/pp.47.4.457. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dreyfuss B. W., Thornber J. P. Assembly of the Light-Harvesting Complexes (LHCs) of Photosystem II (Monomeric LHC IIb Complexes Are Intermediates in the Formation of Oligomeric LHC IIb Complexes). Plant Physiol. 1994 Nov;106(3):829–839. doi: 10.1104/pp.106.3.829. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Duysen M. E., Freeman T. P., Williams N. D., Huckle L. L. Chloramphenicol stimulation of light harvesting chlorophyll protein complex accumulation in a chlorophyll B deficient wheat mutant. Plant Physiol. 1985 Jul;78(3):531–536. doi: 10.1104/pp.78.3.531. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Falbel T. G., Staehelin L. A. Characterization of a family of chlorophyll-deficient wheat (Triticum) and barley (Hordeum vulgare) mutants with defects in the magnesium-insertion step of chlorophyll biosynthesis. Plant Physiol. 1994 Feb;104(2):639–648. doi: 10.1104/pp.104.2.639. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Greene B. A., Allred D. R., Morishige D. T., Staehelin L. A. Hierarchical Response of Light Harvesting Chlorophyll-Proteins in a Light-Sensitive Chlorophyll b-Deficient Mutant of Maize. Plant Physiol. 1988 Jun;87(2):357–364. doi: 10.1104/pp.87.2.357. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Homann P. H., Schmid G. H. Photosynthetic reactions of chloroplasts with unusual structures. Plant Physiol. 1967 Nov;42(11):1619–1632. doi: 10.1104/pp.42.11.1619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Król M., Spangfort M. D., Huner N. P., Oquist G., Gustafsson P., Jansson S. Chlorophyll a/b-binding proteins, pigment conversions, and early light-induced proteins in a chlorophyll b-less barley mutant. Plant Physiol. 1995 Mar;107(3):873–883. doi: 10.1104/pp.107.3.873. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kühlbrandt W., Wang D. N., Fujiyoshi Y. Atomic model of plant light-harvesting complex by electron crystallography. Nature. 1994 Feb 17;367(6464):614–621. doi: 10.1038/367614a0. [DOI] [PubMed] [Google Scholar]
  13. Markwell J., Osterman J. C. Occurrence of Temperature-Sensitive Phenotypic Plasticity in Chlorophyll-Deficient Mutants of Arabidopsis thaliana. Plant Physiol. 1992 Jan;98(1):392–394. doi: 10.1104/pp.98.1.392. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mullet J. E., Burke J. J., Arntzen C. J. Chlorophyll proteins of photosystem I. Plant Physiol. 1980 May;65(5):814–822. doi: 10.1104/pp.65.5.814. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Murray D. L., Kohorn B. D. Chloroplasts of Arabidopsis thaliana homozygous for the ch-1 locus lack chlorophyll b, lack stable LHCPII and have stacked thylakoids. Plant Mol Biol. 1991 Jan;16(1):71–79. doi: 10.1007/BF00017918. [DOI] [PubMed] [Google Scholar]
  16. Plumley F. G., Schmidt G. W. Reconstitution of chlorophyll a/b light-harvesting complexes: Xanthophyll-dependent assembly and energy transfer. Proc Natl Acad Sci U S A. 1987 Jan;84(1):146–150. doi: 10.1073/pnas.84.1.146. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Porra R. J., Schäfer W., Cmiel E., Katheder I., Scheer H. The derivation of the formyl-group oxygen of chlorophyll b in higher plants from molecular oxygen. Achievement of high enrichment of the 7-formyl-group oxygen from 18O2 in greening maize leaves. Eur J Biochem. 1994 Jan 15;219(1-2):671–679. doi: 10.1111/j.1432-1033.1994.tb19983.x. [DOI] [PubMed] [Google Scholar]
  18. Sigrist M., Staehelin L. A. Appearance of type 1, 2, and 3 light-harvesting complex II and light-harvesting complex I proteins during light-induced greening of barley (Hordeum vulgare) etioplasts. Plant Physiol. 1994 Jan;104(1):135–145. doi: 10.1104/pp.104.1.135. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sigrist M., Staehelin L. A. Identification of type 1 and type 2 light-harvesting chlorophyll a/b-binding proteins using monospecific antibodies. Biochim Biophys Acta. 1992 Jan 16;1098(2):191–200. doi: 10.1016/s0005-2728(05)80336-6. [DOI] [PubMed] [Google Scholar]
  20. Wyckoff M., Rodbard D., Chrambach A. Polyacrylamide gel electrophoresis in sodium dodecyl sulfate-containing buffers using multiphasic buffer systems: properties of the stack, valid Rf- measurement, and optimized procedure. Anal Biochem. 1977 Apr;78(2):459–482. doi: 10.1016/0003-2697(77)90107-5. [DOI] [PubMed] [Google Scholar]

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