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. 1997 Feb;113(2):657–661. doi: 10.1104/pp.113.2.657

Molecular Genetic Evidence of the Ability of Alternative Oxidase to Support Respiratory Carbon Metabolism.

G C Vanlerberghe 1, A E Vanlerberghe 1, L McIntosh 1
PMCID: PMC158182  PMID: 12223633

Abstract

With the cytochrome pathway inhibited, AOX was able to support considerable growth of cultured tobacco (Nicotiana tabacum cv Petit Havana SR1) cells but the efficiency of carbon utilization decreased dramatically. Antisense cells with decreased AOX protein did not grow, whereas sense cells with elevated AOX protein had higher growth and respiration rates than the wild type. In antisense cells a large accumulation of pyruvate resulted in aerobic ethanolic fermentation.

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Selected References

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  1. Day D. A., Millar A. H., Wiskich J. T., Whelan J. Regulation of Alternative Oxidase Activity by Pyruvate in Soybean Mitochondria. Plant Physiol. 1994 Dec;106(4):1421–1427. doi: 10.1104/pp.106.4.1421. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Jolivet Y., Pireaux J. C., Dizengremel P. Changes in Properties of Barley Leaf Mitochondria Isolated from NaCl-Treated Plants. Plant Physiol. 1990 Oct;94(2):641–646. doi: 10.1104/pp.94.2.641. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Larson E., Howlett B., Jagendorf A. Artificial reductant enhancement of the Lowry method for protein determination. Anal Biochem. 1986 Jun;155(2):243–248. doi: 10.1016/0003-2697(86)90432-x. [DOI] [PubMed] [Google Scholar]
  4. McIntosh L. Molecular biology of the alternative oxidase. Plant Physiol. 1994 Jul;105(3):781–786. doi: 10.1104/pp.105.3.781. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Millar A. H., Wiskich J. T., Whelan J., Day D. A. Organic acid activation of the alternative oxidase of plant mitochondria. FEBS Lett. 1993 Aug 30;329(3):259–262. doi: 10.1016/0014-5793(93)80233-k. [DOI] [PubMed] [Google Scholar]
  6. Palet A., Ribas-Carbó M., Argilés J. M., Azcón-Bieto J. Short-term effects of carbon dioxide on carnation callus cell respiration. Plant Physiol. 1991 Jun;96(2):467–472. doi: 10.1104/pp.96.2.467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Prasad T. K., Anderson M. D., Stewart C. R. Acclimation, Hydrogen Peroxide, and Abscisic Acid Protect Mitochondria against Irreversible Chilling Injury in Maize Seedlings. Plant Physiol. 1994 Jun;105(2):619–627. doi: 10.1104/pp.105.2.619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Schnapp S. R., Bressan R. A., Hasegawa P. M. Carbon Use Efficiency and Cell Expansion of NaCl-Adapted Tobacco Cells. Plant Physiol. 1990 Jun;93(2):384–388. doi: 10.1104/pp.93.2.384. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Siedow J. N., Umbach A. L. Plant Mitochondrial Electron Transfer and Molecular Biology. Plant Cell. 1995 Jul;7(7):821–831. doi: 10.1105/tpc.7.7.821. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Umbach A. L., Siedow J. N. Covalent and Noncovalent Dimers of the Cyanide-Resistant Alternative Oxidase Protein in Higher Plant Mitochondria and Their Relationship to Enzyme Activity. Plant Physiol. 1993 Nov;103(3):845–854. doi: 10.1104/pp.103.3.845. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Vanlerberghe G. C., McIntosh L. Lower growth temperature increases alternative pathway capacity and alternative oxidase protein in tobacco. Plant Physiol. 1992 Sep;100(1):115–119. doi: 10.1104/pp.100.1.115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Vanlerberghe G. C., McIntosh L. Mitochondrial electron transport regulation of nuclear gene expression. Studies with the alternative oxidase gene of tobacco. Plant Physiol. 1994 Jul;105(3):867–874. doi: 10.1104/pp.105.3.867. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Vanlerberghe G. C., Vanlerberghe A. E., McIntosh L. Molecular Genetic Alteration of Plant Respiration (Silencing and Overexpression of Alternative Oxidase in Transgenic Tobacco). Plant Physiol. 1994 Dec;106(4):1503–1510. doi: 10.1104/pp.106.4.1503. [DOI] [PMC free article] [PubMed] [Google Scholar]

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