Abstract
Low-temperature emission spectra and excitation spectra for chlorophyll fluorescence were recorded from leaves of species of the genus Flaveria (Asteraceae) with C3, C3-C4-intermediate, C4-like, and C4 photosynthesis. Among the latter two groups, high chlorophyll b absorption was observed in excitation spectra for photosystem I (PSI) fluorescence. By comparing leaf data with those from isolated chloroplast fractions, the high chlorophyll b absorption was attributed to the specific properties of the bundle-sheath chloroplasts in leaves from C4 plants. The deconvolution of the PSI excitation spectra and the use of a model revealed that the contribution of photosystem II absorption to the functional antenna of PSI was markedly increased in leaves from three of the five C4-like and C4 species investigated in detail. The two other species exhibited normal, C3-like light-harvesting properties of PSI. The former species are known for efficient carbon assimilation, the latter for decreased efficiencies of carbon assimilation. It is concluded that photosystem II becomes a substantial part of the functional PSI antenna late in the evolution of C4 photosynthesis, and that the composite antenna optimizes the light-harvesting of PSI in bundle-sheath chloroplasts to meet the energy requirements of C4 photosynthesis.
Full Text
The Full Text of this article is available as a PDF (865.6 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Ku M. S., Wu J., Dai Z., Scott R. A., Chu C., Edwards G. E. Photosynthetic and photorespiratory characteristics of flaveria species. Plant Physiol. 1991 Jun;96(2):518–528. doi: 10.1104/pp.96.2.518. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pfundel E., Nagel E., Meister A. Analyzing the Light Energy Distribution in the Photosynthetic Apparatus of C4 Plants Using Highly Purified Mesophyll and Bundle-Sheath Thylakoids. Plant Physiol. 1996 Nov;112(3):1055–1070. doi: 10.1104/pp.112.3.1055. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pfündel E., Meister A. Flow cytometry of mesophyll and bundle sheath chloroplast thylakoids of maize (Zea mays L.). Cytometry. 1996 Feb 1;23(2):97–105. doi: 10.1002/(SICI)1097-0320(19960201)23:2<97::AID-CYTO2>3.0.CO;2-I. [DOI] [PubMed] [Google Scholar]
- Rawsthorne S. Towards an understanding of C3-C4 photosynthesis. Essays Biochem. 1992;27:135–146. [PubMed] [Google Scholar]
- Ruban A. V., Horton P., Robert B. Resonance Raman spectroscopy of the photosystem II light-harvesting complex of green plants: a comparison of trimeric and aggregated states. Biochemistry. 1995 Feb 21;34(7):2333–2337. doi: 10.1021/bi00007a029. [DOI] [PubMed] [Google Scholar]
- Staehelin L. A., Arntzen C. J. Regulation of chloroplast membrane function: protein phosphorylation changes the spatial organization of membrane components. J Cell Biol. 1983 Nov;97(5 Pt 1):1327–1337. doi: 10.1083/jcb.97.5.1327. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Trissl H. W., Wilhelm C. Why do thylakoid membranes from higher plants form grana stacks? Trends Biochem Sci. 1993 Nov;18(11):415–419. doi: 10.1016/0968-0004(93)90136-b. [DOI] [PubMed] [Google Scholar]
- Weis E. Short term acclimation of spinach to high temperatures: effect on chlorophyll fluorescence at 293 and 77 Kelvin in intact leaves. Plant Physiol. 1984 Feb;74(2):402–407. doi: 10.1104/pp.74.2.402. [DOI] [PMC free article] [PubMed] [Google Scholar]