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. 1997 May;114(1):373–381. doi: 10.1104/pp.114.1.373

Pectin Modification in Cell Walls of Ripening Tomatoes Occurs in Distinct Domains.

N M Steele 1, M C McCann 1, K Roberts 1
PMCID: PMC158313  PMID: 12223710

Abstract

The class of cell wall polysaccharides that undergoes the most extensive modification during tomato (Lycopersicon esculentum) fruit ripening is pectin. De-esterification of the polygalacturonic acid backbone by pectin methylesterase facilitates the depolymerization of pectins by polygalacturonase II (PGII). To investigate the spatial aspects of the de-esterification of cell wall pectins and the subsequent deposition of PGII, we have used antibodies to relatively methylesterified and nonesterified pectic epitopes and to the PGII protein on thin sections of pericarp tissue at different developmental stages. De-esterification of pectins and deposition of PGII protein occur in block-like domains within the cell wall. The boundaries of these domains are distinct and persistent, implying strict, spatial regulation of enzymic activities. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of proteins strongly associated with cell walls of pericarp tissue at each stage of fruit development show ripening-related changes in this protein population. Western blots of these gels with anti-PGII antiserum demonstrate that PGII expression is ripening-related. The PGII co-extracts with specific pectic fractions extracted with imidazole or with Na2CO3 at 0[deg]C from the walls of red-ripe pericarp tissue, indicating that the strong association between PGII and the cell wall involves binding to particular pectic polysaccharides.

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Selected References

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  1. Biggs M. S., Handa A. K. Temporal regulation of polygalacturonase gene expression in fruits of normal, mutant, and heterozygous tomato genotypes. Plant Physiol. 1989 Jan;89(1):117–125. doi: 10.1104/pp.89.1.117. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dellapenna D., Lashbrook C. C., Toenjes K., Giovannoni J. J., Fischer R. L., Bennett A. B. Polygalacturonase Isozymes and Pectin Depolymerization in Transgenic rin Tomato Fruit. Plant Physiol. 1990 Dec;94(4):1882–1886. doi: 10.1104/pp.94.4.1882. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Huber D. J., Lee J. H. Uronic Acid products release from enzymically active cell wall from tomato fruit and its dependency on enzyme quantity and distribution. Plant Physiol. 1988 Jul;87(3):592–597. doi: 10.1104/pp.87.3.592. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kobayashi M., Matoh T., Azuma Ji. Two Chains of Rhamnogalacturonan II Are Cross-Linked by Borate-Diol Ester Bonds in Higher Plant Cell Walls. Plant Physiol. 1996 Mar;110(3):1017–1020. doi: 10.1104/pp.110.3.1017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Koch J. L., Nevins D. J. Tomato fruit cell wall : I. Use of purified tomato polygalacturonase and pectinmethylesterase to identify developmental changes in pectins. Plant Physiol. 1989 Nov;91(3):816–822. doi: 10.1104/pp.91.3.816. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  7. Mitcham E. J., Gross K. C., Ng T. J. Tomato Fruit Cell Wall Synthesis during Development and Senescence : In Vivo Radiolabeling of Wall Fractions Using [C]Sucrose. Plant Physiol. 1989 Feb;89(2):477–481. doi: 10.1104/pp.89.2.477. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Moore T., Bennett A. B. Tomato Fruit Polygalacturonase Isozyme 1 (Characterization of the [beta] Subunit and Its State of Assembly in Vivo). Plant Physiol. 1994 Dec;106(4):1461–1469. doi: 10.1104/pp.106.4.1461. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Osteryoung K. W., Toenjes K., Hall B., Winkler V., Bennett A. B. Analysis of tomato polygalacturonase expression in transgenic tobacco. Plant Cell. 1990 Dec;2(12):1239–1248. doi: 10.1105/tpc.2.12.1239. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Selvendran R. R. The plant cell wall as a source of dietary fiber: chemistry and structure. Am J Clin Nutr. 1984 Feb;39(2):320–337. doi: 10.1093/ajcn/39.2.320. [DOI] [PubMed] [Google Scholar]
  11. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Tucker G. A., Robertson N. G., Grierson D. Changes in polygalacturonase isoenzymes during the 'ripening' of normal and mutant tomato fruit. Eur J Biochem. 1980 Nov;112(1):119–124. doi: 10.1111/j.1432-1033.1980.tb04993.x. [DOI] [PubMed] [Google Scholar]

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