Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1997 Jun;114(2):405–410. doi: 10.1104/pp.114.2.405

Mechanism of Protein Transport across the Chloroplast Envelope.

B Fuks 1, D J Schnell 1
PMCID: PMC158319  PMID: 12223715

Full Text

The Full Text of this article is available as a PDF (665.0 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cline K., Henry R. Import and routing of nucleus-encoded chloroplast proteins. Annu Rev Cell Dev Biol. 1996;12:1–26. doi: 10.1146/annurev.cellbio.12.1.1. [DOI] [PubMed] [Google Scholar]
  2. Cline K., Werner-Washburne M., Lubben T. H., Keegstra K. Precursors to two nuclear-encoded chloroplast proteins bind to the outer envelope membrane before being imported into chloroplasts. J Biol Chem. 1985 Mar 25;260(6):3691–3696. [PubMed] [Google Scholar]
  3. Dobberstein B., Blobel G., Chua N. H. In vitro synthesis and processing of a putative precursor for the small subunit of ribulose-1,5-bisphosphate carboxylase of Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A. 1977 Mar;74(3):1082–1085. doi: 10.1073/pnas.74.3.1082. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Friedman A. L., Keegstra K. Chloroplast protein import : quantitative analysis of precursor binding. Plant Physiol. 1989 Mar;89(3):993–999. doi: 10.1104/pp.89.3.993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Guéra A., America T., van Waas M., Weisbeek P. J. A strong protein unfolding activity is associated with the binding of precursor chloroplast proteins to chloroplast envelopes. Plant Mol Biol. 1993 Oct;23(2):309–324. doi: 10.1007/BF00029007. [DOI] [PubMed] [Google Scholar]
  6. Hirsch S., Muckel E., Heemeyer F., von Heijne G., Soll J. A receptor component of the chloroplast protein translocation machinery. Science. 1994 Dec 23;266(5193):1989–1992. doi: 10.1126/science.7801125. [DOI] [PubMed] [Google Scholar]
  7. Kessler F., Blobel G. Interaction of the protein import and folding machineries of the chloroplast. Proc Natl Acad Sci U S A. 1996 Jul 23;93(15):7684–7689. doi: 10.1073/pnas.93.15.7684. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kessler F., Blobel G., Patel H. A., Schnell D. J. Identification of two GTP-binding proteins in the chloroplast protein import machinery. Science. 1994 Nov 11;266(5187):1035–1039. doi: 10.1126/science.7973656. [DOI] [PubMed] [Google Scholar]
  9. Ko K., Budd D., Wu C., Seibert F., Kourtz L., Ko Z. W. Isolation and characterization of a cDNA clone encoding a member of the Com44/Cim44 envelope components of the chloroplast protein import apparatus. J Biol Chem. 1995 Dec 1;270(48):28601–28608. doi: 10.1074/jbc.270.48.28601. [DOI] [PubMed] [Google Scholar]
  10. Lübeck J., Soll J., Akita M., Nielsen E., Keegstra K. Topology of IEP110, a component of the chloroplastic protein import machinery present in the inner envelope membrane. EMBO J. 1996 Aug 15;15(16):4230–4238. [PMC free article] [PubMed] [Google Scholar]
  11. Ma Y., Kouranov A., LaSala S. E., Schnell D. J. Two components of the chloroplast protein import apparatus, IAP86 and IAP75, interact with the transit sequence during the recognition and translocation of precursor proteins at the outer envelope. J Cell Biol. 1996 Jul;134(2):315–327. doi: 10.1083/jcb.134.2.315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Marshall J. S., Keegstra K. Isolation and characterization of a cDNA clone encoding the major hsp70 of the pea chloroplastic stroma. Plant Physiol. 1992 Oct;100(2):1048–1054. doi: 10.1104/pp.100.2.1048. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nielsen E., Akita M., Davila-Aponte J., Keegstra K. Stable association of chloroplastic precursors with protein translocation complexes that contain proteins from both envelope membranes and a stromal Hsp100 molecular chaperone. EMBO J. 1997 Mar 3;16(5):935–946. doi: 10.1093/emboj/16.5.935. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Olsen L. J., Keegstra K. The binding of precursor proteins to chloroplasts requires nucleoside triphosphates in the intermembrane space. J Biol Chem. 1992 Jan 5;267(1):433–439. [PubMed] [Google Scholar]
  15. Pain D., Blobel G. Protein import into chloroplasts requires a chloroplast ATPase. Proc Natl Acad Sci U S A. 1987 May;84(10):3288–3292. doi: 10.1073/pnas.84.10.3288. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Perry S. E., Keegstra K. Envelope membrane proteins that interact with chloroplastic precursor proteins. Plant Cell. 1994 Jan;6(1):93–105. doi: 10.1105/tpc.6.1.93. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Pilon M., Rietveld A. G., Weisbeek P. J., de Kruijff B. Secondary structure and folding of a functional chloroplast precursor protein. J Biol Chem. 1992 Oct 5;267(28):19907–19913. [PubMed] [Google Scholar]
  18. Schatz G., Dobberstein B. Common principles of protein translocation across membranes. Science. 1996 Mar 15;271(5255):1519–1526. doi: 10.1126/science.271.5255.1519. [DOI] [PubMed] [Google Scholar]
  19. Schnell D. J., Blobel G. Identification of intermediates in the pathway of protein import into chloroplasts and their localization to envelope contact sites. J Cell Biol. 1993 Jan;120(1):103–115. doi: 10.1083/jcb.120.1.103. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Schnell D. J., Kessler F., Blobel G. Isolation of components of the chloroplast protein import machinery. Science. 1994 Nov 11;266(5187):1007–1012. doi: 10.1126/science.7973649. [DOI] [PubMed] [Google Scholar]
  21. Scott S. V., Theg S. M. A new chloroplast protein import intermediate reveals distinct translocation machineries in the two envelope membranes: energetics and mechanistic implications. J Cell Biol. 1996 Jan;132(1-2):63–75. doi: 10.1083/jcb.132.1.63. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Seedorf M., Waegemann K., Soll J. A constituent of the chloroplast import complex represents a new type of GTP-binding protein. Plant J. 1995 Mar;7(3):401–411. doi: 10.1046/j.1365-313x.1995.7030401.x. [DOI] [PubMed] [Google Scholar]
  23. Theg S. M., Bauerle C., Olsen L. J., Selman B. R., Keegstra K. Internal ATP is the only energy requirement for the translocation of precursor proteins across chloroplastic membranes. J Biol Chem. 1989 Apr 25;264(12):6730–6736. [PubMed] [Google Scholar]
  24. VanderVere P. S., Bennett T. M., Oblong J. E., Lamppa G. K. A chloroplast processing enzyme involved in precursor maturation shares a zinc-binding motif with a recently recognized family of metalloendopeptidases. Proc Natl Acad Sci U S A. 1995 Aug 1;92(16):7177–7181. doi: 10.1073/pnas.92.16.7177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Walker D., Chaddock A. M., Chaddock J. A., Roberts L. M., Lord J. M., Robinson C. Ricin A chain fused to a chloroplast-targeting signal is unfolded on the chloroplast surface prior to import across the envelope membranes. J Biol Chem. 1996 Feb 23;271(8):4082–4085. doi: 10.1074/jbc.271.8.4082. [DOI] [PubMed] [Google Scholar]
  26. Wu C., Seibert F. S., Ko K. Identification of chloroplast envelope proteins in close physical proximity to a partially translocated chimeric precursor protein. J Biol Chem. 1994 Dec 23;269(51):32264–32271. [PubMed] [Google Scholar]
  27. van 't Hof R., de Kruijff B. Transit sequence-dependent binding of the chloroplast precursor protein ferredoxin to lipid vesicles and its implications for membrane stability. FEBS Lett. 1995 Mar 13;361(1):35–40. doi: 10.1016/0014-5793(95)00135-v. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES