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. 1997 Jun;114(2):483–491. doi: 10.1104/pp.114.2.483

Does Decrease in Ribulose-1,5-Bisphosphate Carboxylase by Antisense RbcS Lead to a Higher N-Use Efficiency of Photosynthesis under Conditions of Saturating CO2 and Light in Rice Plants?

A Makino 1, T Shimada 1, S Takumi 1, K Kaneko 1, M Matsuoka 1, K Shimamoto 1, H Nakano 1, M Miyao-Tokutomi 1, T Mae 1, N Yamamoto 1
PMCID: PMC158328  PMID: 12223722

Abstract

Rice (Oryza sativa L.) plants with decreased ribulose-1,5-bisphosphate carboxylase (Rubisco) were obtained by transformation with the rice rbcS antisense gene under the control of the rice rbcS promoter. The primary transformants were screened for the Rubisco to leaf N ratio, and the transformant with 65% wild-type Rubisco was selected as a plant set with optimal Rubisco content at saturating CO2 partial pressures for photosynthesis under conditions of high irradiance and 25[deg]C. This optimal Rubisco content was estimated from the amounts and kinetic constants of Rubisco and the gas-exchange data. The R1 selfed progeny of the selected transformant were grown hydroponically with different N concentrations. Rubisco content in the R1 population was distributed into two groups: 56 plants had about 65% wild-type Rubisco, whereas 23 plants were very similar to the wild type. Although the plants with decreased Rubisco showed 20% lower rates of light-saturated photosynthesis in normal air (36 Pa CO2), they had 5 to 15% higher rates of photosynthesis in elevated partial pressures of CO2, (100-115 Pa CO2) than the wild-type plants for a given leaf N content. We conclude that the rice plants with 65% wild-type Rubisco show a higher N-use efficiency of photosynthesis under conditions of saturating CO2 and high irradiance.

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Selected References

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  1. Furbank R. T., Chitty J. A., Von Caemmerer S., Jenkins CLD. Antisense RNA Inhibition of RbcS Gene Expression Reduces Rubisco Level and Photosynthesis in the C4 Plant Flaveria bidentis. Plant Physiol. 1996 Jul;111(3):725–734. doi: 10.1104/pp.111.3.725. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Furbank R. T., Taylor W. C. Regulation of Photosynthesis in C3 and C4 Plants: A Molecular Approach. Plant Cell. 1995 Jul;7(7):797–807. doi: 10.1105/tpc.7.7.797. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hidema J., Makino A., Mae T., Ojima K. Photosynthetic Characteristics of Rice Leaves Aged under Different Irradiances from Full Expansion through Senescence. Plant Physiol. 1991 Dec;97(4):1287–1293. doi: 10.1104/pp.97.4.1287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hudson G. S., Evans J. R., von Caemmerer S., Arvidsson Y. B., Andrews T. J. Reduction of ribulose-1,5-bisphosphate carboxylase/oxygenase content by antisense RNA reduces photosynthesis in transgenic tobacco plants. Plant Physiol. 1992 Jan;98(1):294–302. doi: 10.1104/pp.98.1.294. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ichihara Y., Kurosawa Y. Construction of new T vectors for direct cloning of PCR products. Gene. 1993 Aug 16;130(1):153–154. doi: 10.1016/0378-1119(93)90361-6. [DOI] [PubMed] [Google Scholar]
  6. Kyozuka J., McElroy D., Hayakawa T., Xie Y., Wu R., Shimamoto K. Light-regulated and cell-specific expression of tomato rbcS-gusA and rice rbcS-gusA fusion genes in transgenic rice. Plant Physiol. 1993 Jul;102(3):991–1000. doi: 10.1104/pp.102.3.991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Makino A., Nakano H., Mae T. Effects of Growth Temperature on the Responses of Ribulose-1,5-Biphosphate Carboxylase, Electron Transport Components, and Sucrose Synthesis Enzymes to Leaf Nitrogen in Rice, and Their Relationships to Photosynthesis. Plant Physiol. 1994 Aug;105(4):1231–1238. doi: 10.1104/pp.105.4.1231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Makino A., Nakano H., Mae T. Responses of Ribulose-1,5-Bisphosphate Carboxylase, Cytochrome f, and Sucrose Synthesis Enzymes in Rice Leaves to Leaf Nitrogen and Their Relationships to Photosynthesis. Plant Physiol. 1994 May;105(1):173–179. doi: 10.1104/pp.105.1.173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Makino A., Sakashita H., Hidema J., Mae T., Ojima K., Osmond B. Distinctive Responses of Ribulose-1,5-Bisphosphate Carboxylase and Carbonic Anhydrase in Wheat Leaves to Nitrogen Nutrition and their Possible Relationships to CO(2)-Transfer Resistance. Plant Physiol. 1992 Dec;100(4):1737–1743. doi: 10.1104/pp.100.4.1737. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Masle J., Hudson G. S., Badger M. R. Effects of Ambient CO2 Concentration on Growth and Nitrogen Use in Tobacco (Nicotiana tabacum) Plants Transformed with an Antisense Gene to the Small Subunit of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase. Plant Physiol. 1993 Dec;103(4):1075–1088. doi: 10.1104/pp.103.4.1075. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mott K. A., Jensen R. G., O'leary J. W., Berry J. A. Photosynthesis and Ribulose 1,5-Bisphosphate Concentrations in Intact Leaves of Xanthium strumarium L. Plant Physiol. 1984 Dec;76(4):968–971. doi: 10.1104/pp.76.4.968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Plumley F. G., Schmidt G. W. Rocket and crossed immunoelectrophoresis of proteins solubilized with sodium dodecyl sulfate. Anal Biochem. 1983 Oct 1;134(1):86–95. doi: 10.1016/0003-2697(83)90267-1. [DOI] [PubMed] [Google Scholar]
  13. Register J. C., 3rd, Peterson D. J., Bell P. J., Bullock W. P., Evans I. J., Frame B., Greenland A. J., Higgs N. S., Jepson I., Jiao S. Structure and function of selectable and non-selectable transgenes in maize after introduction by particle bombardment. Plant Mol Biol. 1994 Sep;25(6):951–961. doi: 10.1007/BF00014669. [DOI] [PubMed] [Google Scholar]
  14. Rodermel S. R., Abbott M. S., Bogorad L. Nuclear-organelle interactions: nuclear antisense gene inhibits ribulose bisphosphate carboxylase enzyme levels in transformed tobacco plants. Cell. 1988 Nov 18;55(4):673–681. doi: 10.1016/0092-8674(88)90226-7. [DOI] [PubMed] [Google Scholar]
  15. Sage R. F., Sharkey T. D., Seemann J. R. Regulation of Ribulose-1,5-Bisphosphate Carboxylase Activity in Response to Light Intensity and CO(2) in the C(3) Annuals Chenopodium album L. and Phaseolus vulgaris L. Plant Physiol. 1990 Dec;94(4):1735–1742. doi: 10.1104/pp.94.4.1735. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sharkey T. D., Stitt M., Heineke D., Gerhardt R., Raschke K., Heldt H. W. Limitation of Photosynthesis by Carbon Metabolism : II. O(2)-Insensitive CO(2) Uptake Results from Limitation Of Triose Phosphate Utilization. Plant Physiol. 1986 Aug;81(4):1123–1129. doi: 10.1104/pp.81.4.1123. [DOI] [PMC free article] [PubMed] [Google Scholar]

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