Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1997 Aug;114(4):1151–1159. doi: 10.1104/pp.114.4.1151

Patch-clamping and other molecular approaches for the study of plasma membrane transporters demystified.

J M Ward 1
PMCID: PMC158407  PMID: 9276944

Full Text

The Full Text of this article is available as a PDF (963.9 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armstrong F., Leung J., Grabov A., Brearley J., Giraudat J., Blatt M. R. Sensitivity to abscisic acid of guard-cell K+ channels is suppressed by abi1-1, a mutant Arabidopsis gene encoding a putative protein phosphatase. Proc Natl Acad Sci U S A. 1995 Oct 10;92(21):9520–9524. doi: 10.1073/pnas.92.21.9520. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boorer K. J., Frommer W. B., Bush D. R., Kreman M., Loo D. D., Wright E. M. Kinetics and specificity of a H+/amino acid transporter from Arabidopsis thaliana. J Biol Chem. 1996 Jan 26;271(4):2213–2220. doi: 10.1074/jbc.271.4.2213. [DOI] [PubMed] [Google Scholar]
  3. Boorer K. J., Loo D. D., Frommer W. B., Wright E. M. Transport mechanism of the cloned potato H+/sucrose cotransporter StSUT1. J Biol Chem. 1996 Oct 11;271(41):25139–25144. doi: 10.1074/jbc.271.41.25139. [DOI] [PubMed] [Google Scholar]
  4. Cho M. H., Spalding E. P. An anion channel in Arabidopsis hypocotyls activated by blue light. Proc Natl Acad Sci U S A. 1996 Jul 23;93(15):8134–8138. doi: 10.1073/pnas.93.15.8134. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gassman W., Rubio F., Schroeder J. I. Alkali cation selectivity of the wheat root high-affinity potassium transporter HKT1. Plant J. 1996 Nov;10(5):869–852. doi: 10.1046/j.1365-313x.1996.10050869.x. [DOI] [PubMed] [Google Scholar]
  6. Hamill O. P., Marty A., Neher E., Sakmann B., Sigworth F. J. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch. 1981 Aug;391(2):85–100. doi: 10.1007/BF00656997. [DOI] [PubMed] [Google Scholar]
  7. Henriksen G. H., Taylor A. R., Brownlee C., Assmann S. M. Laser microsurgery of higher plant cell walls permits patch-clamp access. Plant Physiol. 1996;110:1063–1068. doi: 10.1104/pp.110.4.1063. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hsu L. C., Chiou T. J., Chen L., Bush D. R. Cloning a plant amino acid transporter by functional complementation of a yeast amino acid transport mutant. Proc Natl Acad Sci U S A. 1993 Aug 15;90(16):7441–7445. doi: 10.1073/pnas.90.16.7441. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ketchum K. A., Slayman C. W. Isolation of an ion channel gene from Arabidopsis thaliana using the H5 signature sequence from voltage-dependent K+ channels. FEBS Lett. 1996 Jan 2;378(1):19–26. doi: 10.1016/0014-5793(95)01417-9. [DOI] [PubMed] [Google Scholar]
  10. Kinoshita T., Nishimura M., Shimazaki Ki. Cytosolic Concentration of Ca2+ Regulates the Plasma Membrane H+-ATPase in Guard Cells of Fava Bean. Plant Cell. 1995 Aug;7(8):1333–1342. doi: 10.1105/tpc.7.8.1333. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. McKinney E. C., Ali N., Traut A., Feldmann K. A., Belostotsky D. A., McDowell J. M., Meagher R. B. Sequence-based identification of T-DNA insertion mutations in Arabidopsis: actin mutants act2-1 and act4-1. Plant J. 1995 Oct;8(4):613–622. doi: 10.1046/j.1365-313x.1995.8040613.x. [DOI] [PubMed] [Google Scholar]
  12. Ninnemann O., Jauniaux J. C., Frommer W. B. Identification of a high affinity NH4+ transporter from plants. EMBO J. 1994 Aug 1;13(15):3464–3471. doi: 10.1002/j.1460-2075.1994.tb06652.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pei Z. M., Kuchitsu K., Ward J. M., Schwarz M., Schroeder J. I. Differential abscisic acid regulation of guard cell slow anion channels in Arabidopsis wild-type and abi1 and abi2 mutants. Plant Cell. 1997 Mar;9(3):409–423. doi: 10.1105/tpc.9.3.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pusch M., Neher E. Rates of diffusional exchange between small cells and a measuring patch pipette. Pflugers Arch. 1988 Feb;411(2):204–211. doi: 10.1007/BF00582316. [DOI] [PubMed] [Google Scholar]
  15. Rentsch D., Laloi M., Rouhara I., Schmelzer E., Delrot S., Frommer W. B. NTR1 encodes a high affinity oligopeptide transporter in Arabidopsis. FEBS Lett. 1995 Aug 21;370(3):264–268. doi: 10.1016/0014-5793(95)00853-2. [DOI] [PubMed] [Google Scholar]
  16. Riesmeier J. W., Willmitzer L., Frommer W. B. Evidence for an essential role of the sucrose transporter in phloem loading and assimilate partitioning. EMBO J. 1994 Jan 1;13(1):1–7. doi: 10.1002/j.1460-2075.1994.tb06229.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Riesmeier J. W., Willmitzer L., Frommer W. B. Isolation and characterization of a sucrose carrier cDNA from spinach by functional expression in yeast. EMBO J. 1992 Dec;11(13):4705–4713. doi: 10.1002/j.1460-2075.1992.tb05575.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rubio F., Gassmann W., Schroeder J. I. Sodium-driven potassium uptake by the plant potassium transporter HKT1 and mutations conferring salt tolerance. Science. 1995 Dec 8;270(5242):1660–1663. doi: 10.1126/science.270.5242.1660. [DOI] [PubMed] [Google Scholar]
  19. Schachtman D. P., Schroeder J. I., Lucas W. J., Anderson J. A., Gaber R. F. Expression of an inward-rectifying potassium channel by the Arabidopsis KAT1 cDNA. Science. 1992 Dec 4;258(5088):1654–1658. doi: 10.1126/science.8966547. [DOI] [PubMed] [Google Scholar]
  20. Schachtman D. P., Schroeder J. I. Structure and transport mechanism of a high-affinity potassium uptake transporter from higher plants. Nature. 1994 Aug 25;370(6491):655–658. doi: 10.1038/370655a0. [DOI] [PubMed] [Google Scholar]
  21. Schroeder J. I., Raschke K., Neher E. Voltage dependence of K channels in guard-cell protoplasts. Proc Natl Acad Sci U S A. 1987 Jun;84(12):4108–4112. doi: 10.1073/pnas.84.12.4108. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Schroeder J. I., Ward J. M., Gassmann W. Perspectives on the physiology and structure of inward-rectifying K+ channels in higher plants: biophysical implications for K+ uptake. Annu Rev Biophys Biomol Struct. 1994;23:441–471. doi: 10.1146/annurev.bb.23.060194.002301. [DOI] [PubMed] [Google Scholar]
  23. Sentenac H., Bonneaud N., Minet M., Lacroute F., Salmon J. M., Gaymard F., Grignon C. Cloning and expression in yeast of a plant potassium ion transport system. Science. 1992 May 1;256(5057):663–665. doi: 10.1126/science.1585180. [DOI] [PubMed] [Google Scholar]
  24. Smith F. W., Ealing P. M., Hawkesford M. J., Clarkson D. T. Plant members of a family of sulfate transporters reveal functional subtypes. Proc Natl Acad Sci U S A. 1995 Sep 26;92(20):9373–9377. doi: 10.1073/pnas.92.20.9373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Tsay Y. F., Schroeder J. I., Feldmann K. A., Crawford N. M. The herbicide sensitivity gene CHL1 of Arabidopsis encodes a nitrate-inducible nitrate transporter. Cell. 1993 Mar 12;72(5):705–713. doi: 10.1016/0092-8674(93)90399-b. [DOI] [PubMed] [Google Scholar]
  26. Uozumi N., Gassmann W., Cao Y., Schroeder J. I. Identification of strong modifications in cation selectivity in an Arabidopsis inward rectifying potassium channel by mutant selection in yeast. J Biol Chem. 1995 Oct 13;270(41):24276–24281. doi: 10.1074/jbc.270.41.24276. [DOI] [PubMed] [Google Scholar]
  27. Will A., Caspari T., Tanner W. Km mutants of the Chlorella monosaccharide/H+ cotransporter randomly generated by PCR. Proc Natl Acad Sci U S A. 1994 Oct 11;91(21):10163–10167. doi: 10.1073/pnas.91.21.10163. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES