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. 1997 Aug;114(4):1523–1531. doi: 10.1104/pp.114.4.1523

Cell Wall Metabolism in Ripening Fruit (IX. Synthesis of Pectic and Hemicellulosic Cell Wall Polymers in the Outer Pericarp of Mature Green Tomatoes (cv XMT-22).

M Huysamer 1, L C Greve 1, J M Labavitch 1
PMCID: PMC158446  PMID: 12223785

Abstract

Discs of outer pericarp were excised from mature green tomato (Lycopersicon esculentum Mill.) fruit and kept in sterile tissue culture plates for 4 d, including 2 d of incubation with D-[U-13C]glucose. Cell walls were prepared and the water-soluble, pectic, and hemicellulosic polymers were extracted. Cell wall synthetic capacity was determined by gas chromatography-mass spectrometry analysis of incorporation of the heavy isotope label. The "outer" 2-mm pericarp region, which included the cuticle, had a lower cell wall synthetic capacity than the "inner" 2-mm region immediately below it (closer to the locules), based on the percentage of labeling of the neutral sugars. There were no significant differences in relative abundance of glycosidic linkages in the two tissue regions. Label was incorporated into neutral sugars and linkages typical for each polysaccharide class were identified in the cell wall preparations. Galacturonic acid and glucuronic acid were labeled to an extent similar to that of the neutral sugars in each tissue region.

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Selected References

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  1. Ahmed A. E., Labavitch J. M. Cell Wall Metabolism in Ripening Fruit: I. CELL WALL CHANGES IN RIPENING ;BARTLETT' PEARS. Plant Physiol. 1980 May;65(5):1009–1013. doi: 10.1104/pp.65.5.1009. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baker D. B., Ray P. M. Relation between Effects of Auxin on Cell Wall Synthesis and Cell Elongation. Plant Physiol. 1965 Mar;40(2):360–368. doi: 10.1104/pp.40.2.360. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bhat U. R., Mayer H., Yokota A., Hollingsworth R. I., Carlson R. W. Occurrence of lipid A variants with 27-hydroxyoctacosanoic acid in lipopolysaccharides from members of the family Rhizobiaceae. J Bacteriol. 1991 Apr;173(7):2155–2159. doi: 10.1128/jb.173.7.2155-2159.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Campbell A. D., Huysamer M., Stotz H. U., Greve L. C., Labavitch J. M. Comparison of ripening processes in intact tomato fruit and excised pericarp discs. Plant Physiol. 1990 Dec;94(4):1582–1589. doi: 10.1104/pp.94.4.1582. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Giovannoni J. J., DellaPenna D., Bennett A. B., Fischer R. L. Expression of a chimeric polygalacturonase gene in transgenic rin (ripening inhibitor) tomato fruit results in polyuronide degradation but not fruit softening. Plant Cell. 1989 Jan;1(1):53–63. doi: 10.1105/tpc.1.1.53. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Greve L. C., Labavitch J. M. Cell Wall Metabolism in Ripening Fruit : V. Analysis of Cell Wall Synthesis in Ripening Tomato Pericarp Tissue Using a d-[U-C]Glucose Tracer and Gas Chromatography-Mass Spectrometry. Plant Physiol. 1991 Dec;97(4):1456–1461. doi: 10.1104/pp.97.4.1456. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gross K. C., Wallner S. J. Degradation of Cell Wall Polysaccharides during Tomato Fruit Ripening. Plant Physiol. 1979 Jan;63(1):117–120. doi: 10.1104/pp.63.1.117. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kreuger M., van Holst G. J. Arabinogalactan proteins and plant differentiation. Plant Mol Biol. 1996 Mar;30(6):1077–1086. doi: 10.1007/BF00019543. [DOI] [PubMed] [Google Scholar]
  9. McNeil M., Darvill A. G., Fry S. C., Albersheim P. Structure and function of the primary cell walls of plants. Annu Rev Biochem. 1984;53:625–663. doi: 10.1146/annurev.bi.53.070184.003205. [DOI] [PubMed] [Google Scholar]
  10. Mitcham E. J., Gross K. C., Ng T. J. Tomato Fruit Cell Wall Synthesis during Development and Senescence : In Vivo Radiolabeling of Wall Fractions Using [C]Sucrose. Plant Physiol. 1989 Feb;89(2):477–481. doi: 10.1104/pp.89.2.477. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sheehy R. E., Kramer M., Hiatt W. R. Reduction of polygalacturonase activity in tomato fruit by antisense RNA. Proc Natl Acad Sci U S A. 1988 Dec;85(23):8805–8809. doi: 10.1073/pnas.85.23.8805. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Yelle S., Hewitt J. D., Robinson N. L., Damon S., Bennett A. B. Sink Metabolism in Tomato Fruit : III. Analysis of Carbohydrate Assimilation in a Wild Species. Plant Physiol. 1988 Jul;87(3):737–740. doi: 10.1104/pp.87.3.737. [DOI] [PMC free article] [PubMed] [Google Scholar]

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