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. 1997 Oct;115(2):397–407. doi: 10.1104/pp.115.2.397

A G-Box-Binding Protein from Soybean Binds to the E1 Auxin-Response Element in the Soybean GH3 Promoter and Contains a Proline-Rich Repression Domain.

Z B Liu 1, G Hagen 1, T J Guilfoyle 1
PMCID: PMC158497  PMID: 12223815

Abstract

The E1 promoter fragment (-249 to -203) is one of three auxin-response elements (AuxREs) in the soybean (Glycine max L.) GH3 promoter (Z.-B. Liu, T. Ulmasov, X. Shi, G. Hagen, T.J. Guilfoyle [1994] Plant Cell 6: 645-657). Results presented here further characterize and delimit the AuxRE within the E1 fragment. The E1 fragment functioned as an AuxRE in transgenic tobacco (Nicotiana tabacum L.) plants, as well as in transfected protoplasts. The AuxRE within E1 contains a G-box, and this G-box was used to clone a G-box-binding factor (GBF) from soybean (SGBF-2). This 45-kD GBF contains an N-terminal proline-rich domain and a C-terminal basic/leucine zipper DNA-binding domain. Gel-mobility shift assays were used to characterize the binding specificity of SGBF-2. Antiserum raised against recombinant SGBF-2 was used to further characterize SGBF-2 and antigenically related GBFs in soybean nuclear extracts. Co-transfection assays with effector and reporter plasmids in carrot (Daucus carota L.) protoplasts indicated that the N-terminal proline-rich domain of SGBF-2 functioned as a repression domain in both basal and auxin-inducible transcription.

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Selected References

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  1. Abel S., Theologis A. Early genes and auxin action. Plant Physiol. 1996 May;111(1):9–17. doi: 10.1104/pp.111.1.9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Artandi S. E., Merrell K., Avitahl N., Wong K. K., Calame K. TFE3 contains two activation domains, one acidic and the other proline-rich, that synergistically activate transcription. Nucleic Acids Res. 1995 Oct 11;23(19):3865–3871. doi: 10.1093/nar/23.19.3865. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chern M. S., Bobb A. J., Bustos M. M. The regulator of MAT2 (ROM2) protein binds to early maturation promoters and represses PvALF-activated transcription. Plant Cell. 1996 Feb;8(2):305–321. doi: 10.1105/tpc.8.2.305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chern M. S., Eiben H. G., Bustos M. M. The developmentally regulated bZIP factor ROM1 modulates transcription from lectin and storage protein genes in bean embryos. Plant J. 1996 Jul;10(1):135–148. doi: 10.1046/j.1365-313x.1996.10010135.x. [DOI] [PubMed] [Google Scholar]
  5. Dombroski A. J., Walter W. A., Record M. T., Jr, Siegele D. A., Gross C. A. Polypeptides containing highly conserved regions of transcription initiation factor sigma 70 exhibit specificity of binding to promoter DNA. Cell. 1992 Aug 7;70(3):501–512. doi: 10.1016/0092-8674(92)90174-b. [DOI] [PubMed] [Google Scholar]
  6. Feldbrügge M., Sprenger M., Dinkelbach M., Yazaki K., Harter K., Weisshaar B. Functional analysis of a light-responsive plant bZIP transcriptional regulator. Plant Cell. 1994 Nov;6(11):1607–1621. doi: 10.1105/tpc.6.11.1607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hagen G., Guilfoyle T. J. Rapid induction of selective transcription by auxins. Mol Cell Biol. 1985 Jun;5(6):1197–1203. doi: 10.1128/mcb.5.6.1197. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hagen G., Martin G., Li Y., Guilfoyle T. J. Auxin-induced expression of the soybean GH3 promoter in transgenic tobacco plants. Plant Mol Biol. 1991 Sep;17(3):567–579. doi: 10.1007/BF00040658. [DOI] [PubMed] [Google Scholar]
  9. Izawa T., Foster R., Chua N. H. Plant bZIP protein DNA binding specificity. J Mol Biol. 1993 Apr 20;230(4):1131–1144. doi: 10.1006/jmbi.1993.1230. [DOI] [PubMed] [Google Scholar]
  10. Landschulz W. H., Johnson P. F., McKnight S. L. The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins. Science. 1988 Jun 24;240(4860):1759–1764. doi: 10.1126/science.3289117. [DOI] [PubMed] [Google Scholar]
  11. Laurent B. C., Treitel M. A., Carlson M. The SNF5 protein of Saccharomyces cerevisiae is a glutamine- and proline-rich transcriptional activator that affects expression of a broad spectrum of genes. Mol Cell Biol. 1990 Nov;10(11):5616–5625. doi: 10.1128/mcb.10.11.5616. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Liu Z. B., Ulmasov T., Shi X., Hagen G., Guilfoyle T. J. Soybean GH3 promoter contains multiple auxin-inducible elements. Plant Cell. 1994 May;6(5):645–657. doi: 10.1105/tpc.6.5.645. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Menkens A. E., Schindler U., Cashmore A. R. The G-box: a ubiquitous regulatory DNA element in plants bound by the GBF family of bZIP proteins. Trends Biochem Sci. 1995 Dec;20(12):506–510. doi: 10.1016/s0968-0004(00)89118-5. [DOI] [PubMed] [Google Scholar]
  14. Meshi T., Iwabuchi M. Plant transcription factors. Plant Cell Physiol. 1995 Dec;36(8):1405–1420. [PubMed] [Google Scholar]
  15. Sadowski I., Ptashne M. A vector for expressing GAL4(1-147) fusions in mammalian cells. Nucleic Acids Res. 1989 Sep 25;17(18):7539–7539. doi: 10.1093/nar/17.18.7539. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Singh H., LeBowitz J. H., Baldwin A. S., Jr, Sharp P. A. Molecular cloning of an enhancer binding protein: isolation by screening of an expression library with a recognition site DNA. Cell. 1988 Feb 12;52(3):415–423. doi: 10.1016/s0092-8674(88)80034-5. [DOI] [PubMed] [Google Scholar]
  17. Singh H., Sen R., Baltimore D., Sharp P. A. A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genes. Nature. 1986 Jan 9;319(6049):154–158. doi: 10.1038/319154a0. [DOI] [PubMed] [Google Scholar]
  18. Triezenberg S. J., Kingsbury R. C., McKnight S. L. Functional dissection of VP16, the trans-activator of herpes simplex virus immediate early gene expression. Genes Dev. 1988 Jun;2(6):718–729. doi: 10.1101/gad.2.6.718. [DOI] [PubMed] [Google Scholar]
  19. Ulmasov T., Hagen G., Guilfoyle T. J. ARF1, a transcription factor that binds to auxin response elements. Science. 1997 Jun 20;276(5320):1865–1868. doi: 10.1126/science.276.5320.1865. [DOI] [PubMed] [Google Scholar]
  20. Ulmasov T., Liu Z. B., Hagen G., Guilfoyle T. J. Composite structure of auxin response elements. Plant Cell. 1995 Oct;7(10):1611–1623. doi: 10.1105/tpc.7.10.1611. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Vinson C. R., LaMarco K. L., Johnson P. F., Landschulz W. H., McKnight S. L. In situ detection of sequence-specific DNA binding activity specified by a recombinant bacteriophage. Genes Dev. 1988 Jul;2(7):801–806. doi: 10.1101/gad.2.7.801. [DOI] [PubMed] [Google Scholar]

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