Abstract
Conditioned medium from mesophyll cell-suspension cultures of Zinnia elegans L. has striking effects on cell expansion and tracheary element differentiation when applied to cultures of freshly isolated mesophyll cells. These effects include (a) induction of early cell expansion, (b) delay in differentiation by 48 h or more, (c) reduction in the synchrony of differentiation, and (d) early formation of very large, metaxylem-like tracheary elements. Like reduced osmotic potential and buffering at pH 5.5, conditioned medium appears to have its primary effect on cell expansion. Partial characterization of the expansion-inducing factor indicates that it is heat stable, of low molecular mass, and is resistant to protease. It also binds reversibly to concanavalin A but is not adsorbed by charcoal. We suggest that the secreted factor may be an oligosaccharide involved in the coordination of cell expansion and differentiation and the regulation of the protoxylem-like to metaxylem-like transition in xylogenic suspension cultures.
Full Text
The Full Text of this article is available as a PDF (1.6 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Brower D. L., Hepler P. K. Microtubules and secondary wall deposition in xylem: the effects of isopropyl N-phenylcarbamate. Protoplasma. 1976;87(1-3):91–111. doi: 10.1007/BF01623961. [DOI] [PubMed] [Google Scholar]
- Chen K. H., Miller A. N., Patterson G. W., Cohen J. D. A Rapid and Simple Procedure for Purification of Indole-3-Acetic Acid Prior to GC-SIM-MS Analysis. Plant Physiol. 1988 Mar;86(3):822–825. doi: 10.1104/pp.86.3.822. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Côté F., Hahn M. G. Oligosaccharins: structures and signal transduction. Plant Mol Biol. 1994 Dec;26(5):1379–1411. doi: 10.1007/BF00016481. [DOI] [PubMed] [Google Scholar]
- De Jong A. J., Cordewener J., Lo Schiavo F., Terzi M., Vandekerckhove J., Van Kammen A., De Vries S. C. A carrot somatic embryo mutant is rescued by chitinase. Plant Cell. 1992 Apr;4(4):425–433. doi: 10.1105/tpc.4.4.425. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Eberhard S., Doubrava N., Marfa V., Mohnen D., Southwick A., Darvill A., Albersheim P. Pectic Cell Wall Fragments Regulate Tobacco Thin-Cell-Layer Explant Morphogenesis. Plant Cell. 1989 Aug;1(8):747–755. doi: 10.1105/tpc.1.8.747. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fry S. C., Aldington S., Hetherington P. R., Aitken J. Oligosaccharides as signals and substrates in the plant cell wall. Plant Physiol. 1993 Sep;103(1):1–5. doi: 10.1104/pp.103.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McDougall G. J., Fry S. C. Xyloglucan oligosaccharides promote growth and activate cellulase: evidence for a role of cellulase in cell expansion. Plant Physiol. 1990 Jul;93(3):1042–1048. doi: 10.1104/pp.93.3.1042. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Priem B., Morvan H., Gross K. C. Unconjugated N-glycans as a new class of plant oligosaccharins. Biochem Soc Trans. 1994 May;22(2):398–402. doi: 10.1042/bst0220398. [DOI] [PubMed] [Google Scholar]
- Van Engelen F. A., De Vries S. C. Extracellular proteins in plant embryogenesis. Trends Genet. 1992 Feb;8(2):66–70. doi: 10.1016/0168-9525(92)90352-5. [DOI] [PubMed] [Google Scholar]
- York W. S., Darvill A. G., Albersheim P. Inhibition of 2,4-dichlorophenoxyacetic Acid-stimulated elongation of pea stem segments by a xyloglucan oligosaccharide. Plant Physiol. 1984 Jun;75(2):295–297. doi: 10.1104/pp.75.2.295. [DOI] [PMC free article] [PubMed] [Google Scholar]