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. 1997 Nov;115(3):1009–1020. doi: 10.1104/pp.115.3.1009

Characterization of new gibberellin-responsive semidwarf mutants of arabidopsis.

V M Sponsel 1, F W Schmidt 1, S G Porter 1, M Nakayama 1, S Kohlstruk 1, M Estelle 1
PMCID: PMC158564  PMID: 9390435

Abstract

Chemical mutagenesis of Arabidopsis thaliana (L.) Heynh. yielded four semidwarf mutants, all of which appeared to be gibberellin (GA)-biosynthesis mutants. All four had atypical response profiles to C20-GAs, suggesting that each had impaired 20-oxidation. One mutant, 11.2, was shown to be allelic to ga5 and has been named ga5-2. It had altered metabolism of [14C]GA15 relative to that in wild-type plants and undetectable levels of C19-GAs in young stems, consistent with the known function of GA5 as a stem-expressed GA 20-oxidase. Two mutants (2.1 and 10.3), which had very short inflorescences and siliques, were allelic to each other but not to the known GA-responding mutants, ga1 to ga5. The locus defined by these two mutations is provisionally named GA6 and is purported to encode an inflorescence- and silique-expressed GA 20-oxidase. A double mutant, ga5-2 ga6-2, had an extreme dwarf phenotype with very short siliques. The fourth mutation, 1.1, gave a phenotype like ga5, but was not allelic to any of the known ga mutations. It has not yet been given a gene symbol pending further studies.

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Selected References

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  1. Ait-Ali T., Swain S. M., Reid J. B., Sun T., Kamiya Y. The LS locus of pea encodes the gibberellin biosynthesis enzyme ent-kaurene synthase A. Plant J. 1997 Mar;11(3):443–454. doi: 10.1046/j.1365-313x.1997.11030443.x. [DOI] [PubMed] [Google Scholar]
  2. Chiang H. H., Hwang I., Goodman H. M. Isolation of the Arabidopsis GA4 locus. Plant Cell. 1995 Feb;7(2):195–201. doi: 10.1105/tpc.7.2.195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Finkelstein R. R., Somerville C. R. Three Classes of Abscisic Acid (ABA)-Insensitive Mutations of Arabidopsis Define Genes that Control Overlapping Subsets of ABA Responses. Plant Physiol. 1990 Nov;94(3):1172–1179. doi: 10.1104/pp.94.3.1172. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gilmour S. J., Zeevaart J. A., Schwenen L., Graebe J. E. Gibberellin metabolism in cell-free extracts from spinach leaves in relation to photoperiod. Plant Physiol. 1986 Sep;82(1):190–195. doi: 10.1104/pp.82.1.190. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lincoln C., Britton J. H., Estelle M. Growth and development of the axr1 mutants of Arabidopsis. Plant Cell. 1990 Nov;2(11):1071–1080. doi: 10.1105/tpc.2.11.1071. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Martin D. N., Proebsting W. M., Parks T. D., Dougherty W. G., Lange T., Lewis M. J., Gaskin P., Hedden P. Feed-back regulation of gibberellin biosynthesis and gene expression in Pisum sativum L. Planta. 1996;200(2):159–166. doi: 10.1007/BF00208304. [DOI] [PubMed] [Google Scholar]
  7. Nambara E., Akazawa T., McCourt P. Effects of the gibberellin biosynthetic inhibitor uniconazol on mutants of Arabidopsis. Plant Physiol. 1991 Oct;97(2):736–738. doi: 10.1104/pp.97.2.736. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Phillips A. L., Ward D. A., Uknes S., Appleford N. E., Lange T., Huttly A. K., Gaskin P., Graebe J. E., Hedden P. Isolation and expression of three gibberellin 20-oxidase cDNA clones from Arabidopsis. Plant Physiol. 1995 Jul;108(3):1049–1057. doi: 10.1104/pp.108.3.1049. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sponsel V. M., Ross J. J., Reynolds M. R., Symons G. M., Reid J. B. The Gibberellin Status of lip1, a Mutant of Pea That Exhibits Light-Independent Photomorphogenesis. Plant Physiol. 1996 Sep;112(1):61–66. doi: 10.1104/pp.112.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sun T. P., Kamiya Y. The Arabidopsis GA1 locus encodes the cyclase ent-kaurene synthetase A of gibberellin biosynthesis. Plant Cell. 1994 Oct;6(10):1509–1518. doi: 10.1105/tpc.6.10.1509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sun Tp., Goodman H. M., Ausubel F. M. Cloning the Arabidopsis GA1 Locus by Genomic Subtraction. Plant Cell. 1992 Feb;4(2):119–128. doi: 10.1105/tpc.4.2.119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Talon M., Koornneef M., Zeevaart J. A. Endogenous gibberellins in Arabidopsis thaliana and possible steps blocked in the biosynthetic pathways of the semidwarf ga4 and ga5 mutants. Proc Natl Acad Sci U S A. 1990 Oct;87(20):7983–7987. doi: 10.1073/pnas.87.20.7983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Wu K., Li L., Gage D. A., Zeevaart J. A. Molecular cloning and photoperiod-regulated expression of gibberellin 20-oxidase from the long-day plant spinach. Plant Physiol. 1996 Feb;110(2):547–554. doi: 10.1104/pp.110.2.547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Xu Y. L., Li L., Wu K., Peeters A. J., Gage D. A., Zeevaart J. A. The GA5 locus of Arabidopsis thaliana encodes a multifunctional gibberellin 20-oxidase: molecular cloning and functional expression. Proc Natl Acad Sci U S A. 1995 Jul 3;92(14):6640–6644. doi: 10.1073/pnas.92.14.6640. [DOI] [PMC free article] [PubMed] [Google Scholar]

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