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. 1993 Jan;101(1):97–104. doi: 10.1104/pp.101.1.97

Phytochrome levels in the green alga Mesotaenium caldariorum are light regulated.

L Z Morand 1, D G Kidd 1, J C Lagarias 1
PMCID: PMC158652  PMID: 8278502

Abstract

Experiments undertaken in this investigation examine the influence of light on the levels of phytochrome in the green alga Mesotaenium caldariorum and also provide partial protein sequence of the algal phytochrome. Immunochemical and spectrophotometric measurements reveal that phytochrome levels increase nearly 4-fold upon transfer of light-grown algal cells to total darkness during a 6- to 8-d adaptation period. Within 24 h after return to continuous illumination, the level of phytochrome in dark-adapted cells has decreased to that found in light-grown cells. Red or far-red light experiments show that both effects of light, phytochrome accumulation during dark adaptation and light-dependent decrease of phytochrome, do not depend on the form of the phytochrome photoreceptor (i.e. far-red absorbing or red absorbing) present in the algal cell. The light-dependent reduction of phytochrome in dark-adapted cells is inhibited by the photosynthetic electron transport inhibitor 3-(3,4-dichlorophenyl)-1,1-dimethyl urea, suggesting that this light effect is mediated by photosynthesis. Microsequence analyses of internal peptides indicate that algal phytochrome purified from dark-adapted cells shares the greatest sequence identity with phytochrome from the fern Selaginella (74%). Compared with higher plant photoreceptors, Mesotaenium phytochrome appears to be more closely related to phyB gene products (i.e. 62 and 63% average sequence identity) than to phyA gene products (i.e. 50 and 53% average sequence identity). Because light regulation and the structure of Mesotaenium phytochrome do not conform with either type I (light-labile) or type II (light-stable) phytochromes from higher plants, these results support the hypothesis that the lower green plant photoreceptors represent a distinct class of phytochrome.

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Selected References

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  1. Chow W. S., Melis A., Anderson J. M. Adjustments of photosystem stoichiometry in chloroplasts improve the quantum efficiency of photosynthesis. Proc Natl Acad Sci U S A. 1990 Oct;87(19):7502–7506. doi: 10.1073/pnas.87.19.7502. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cordonnier M. M., Greppin H., Pratt L. H. Identification of a highly conserved domain on phytochrome from angiosperms to algae. Plant Physiol. 1986 Apr;80(4):982–987. doi: 10.1104/pp.80.4.982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dehesh K., Tepperman J., Christensen A. H., Quail P. H. phyB is evolutionarily conserved and constitutively expressed in rice seedling shoots. Mol Gen Genet. 1991 Feb;225(2):305–313. doi: 10.1007/BF00269863. [DOI] [PubMed] [Google Scholar]
  4. Furuya M. Molecular properties and biogenesis of phytochrome I and II. Adv Biophys. 1989;25:133–167. doi: 10.1016/0065-227x(89)90006-3. [DOI] [PubMed] [Google Scholar]
  5. Galloway R. E., Mets L. Non-Mendelian Inheritance of 3-(3,4-Dichlorophenyl)-1,1-dimethylurea-Resistant Thylakoid Membrane Properties in Chlamydomonas. Plant Physiol. 1982 Dec;70(6):1673–1677. doi: 10.1104/pp.70.6.1673. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hershey H. P., Barker R. F., Idler K. B., Lissemore J. L., Quail P. H. Analysis of cloned cDNA and genomic sequences for phytochrome: complete amino acid sequences for two gene products expressed in etiolated Avena. Nucleic Acids Res. 1985 Dec 9;13(23):8543–8559. doi: 10.1093/nar/13.23.8543. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kay S. A., Keith B., Shinozaki K., Chua N. H. The sequence of the rice phytochrome gene. Nucleic Acids Res. 1989 Apr 11;17(7):2865–2866. doi: 10.1093/nar/17.7.2865. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kidd D. G., Lagarias J. C. Phytochrome from the green alga Mesotaenium caldariorum. Purification and preliminary characterization. J Biol Chem. 1990 Apr 25;265(12):7029–7035. [PubMed] [Google Scholar]
  9. Quail P. H. Phytochrome: a light-activated molecular switch that regulates plant gene expression. Annu Rev Genet. 1991;25:389–409. doi: 10.1146/annurev.ge.25.120191.002133. [DOI] [PubMed] [Google Scholar]
  10. Schiff J. A., Zeldin M. H., Rubman J. Chlorophyll Formation and Photosynthetic Competence in Euglena During Light-Induced Chloroplast Development in the Presence of 3, (3,4-dichlorophenyl) 1,1-Dimethyl Urea (DCMU). Plant Physiol. 1967 Dec;42(12):1716–1725. doi: 10.1104/pp.42.12.1716. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sheen J. Metabolic repression of transcription in higher plants. Plant Cell. 1990 Oct;2(10):1027–1038. doi: 10.1105/tpc.2.10.1027. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Somers D. E., Sharrock R. A., Tepperman J. M., Quail P. H. The hy3 Long Hypocotyl Mutant of Arabidopsis Is Deficient in Phytochrome B. Plant Cell. 1991 Dec;3(12):1263–1274. doi: 10.1105/tpc.3.12.1263. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Stewart S. J., Pratt L. H., Cordonnier-Pratt I. M. Phytochrome Levels in Light-Grown Avena Change in Response to End-of-Day Irradiations. Plant Physiol. 1992 Aug;99(4):1708–1710. doi: 10.1104/pp.99.4.1708. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Thümmler F., Beetz A., Rüdiger W. Phytochrome in lower plants. Detection and partial sequence of a phytochrome gene in the moss Ceratodon purpureus using the polymerase chain reaction. FEBS Lett. 1990 Nov 26;275(1-2):125–129. doi: 10.1016/0014-5793(90)81455-w. [DOI] [PubMed] [Google Scholar]

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