Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1993 Jan;101(1):201–208. doi: 10.1104/pp.101.1.201

Abolition of an Inducible Highly Anionic Peroxidase Activity in Transgenic Tomato.

B A Sherf 1, A M Bajar 1, P E Kolattukudy 1
PMCID: PMC158665  PMID: 12231677

Abstract

Locally induced expression of a highly anionic peroxidase has previously been correlated temporally and spatially with suberization of tissues responding to pathogen assault, wounding, or exogenously applied abscisic acid or fungal elicitors. DNA sequences corresponding to the 5[prime] regions of two tomato (Lycopersicon esculentum) genes encoding homologous anionic peroxidases were fused, inserted into a pTi-based plasmid designed to express a composite antisense transcript, and introduced into tomato via Agrobacterium-mediated transformation. RNA gel-blot analyses showed high expression of the antisense transcript in most transgenic plants and no detectable induction of native anionic peroxidase transcripts in wounded or abscisic acid or pathogen-treated tissues. Plants and fruits expressing the antisense transcript appeared normal in all respects. Electrophoretic analysis of anionic proteins from selected transgenic plants showed no detectable anionic peroxidase protein or activity. Depolymerization of polymeric material from the wound periderm of transgenic tomato fruits and analysis of the aliphatic products by gas-liquid chromatography/mass spectrometry showed that the content and composition of C16/C18 [omega]-hydroxy and dicarboxylic acids, characteristic of suberin, were not affected by the absence of the anionic peroxidase. Autofluorescence generated from cell wall phenolics at the wound lesion was also not affected by the absence of the highly anionic peroxidase.

Full Text

The Full Text of this article is available as a PDF (2.4 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bevan M. Binary Agrobacterium vectors for plant transformation. Nucleic Acids Res. 1984 Nov 26;12(22):8711–8721. doi: 10.1093/nar/12.22.8711. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dean B. B., Kolattukudy P. E. Synthesis of Suberin during Wound-healing in Jade Leaves, Tomato Fruit, and Bean Pods. Plant Physiol. 1976 Sep;58(3):411–416. doi: 10.1104/pp.58.3.411. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Espelie K. E., Franceschi V. R., Kolattukudy P. E. Immunocytochemical localization and time course of appearance of an anionic peroxidase associated with suberization in wound-healing potato tuber tissue. Plant Physiol. 1986 Jun;81(2):487–492. doi: 10.1104/pp.81.2.487. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. HINMAN R. L., LANG J. PEROXIDASE-CATALYZED OXIDATION OF INDOLE-3-ACETIC ACID. Biochemistry. 1965 Jan;4:144–158. doi: 10.1021/bi00877a023. [DOI] [PubMed] [Google Scholar]
  5. Lagrimini L. M., Burkhart W., Moyer M., Rothstein S. Molecular cloning of complementary DNA encoding the lignin-forming peroxidase from tobacco: Molecular analysis and tissue-specific expression. Proc Natl Acad Sci U S A. 1987 Nov;84(21):7542–7546. doi: 10.1073/pnas.84.21.7542. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Mohan R., Kolattukudy P. E. Differential Activation of Expression of a Suberization-Associated Anionic Peroxidase Gene in Near-Isogenic Resistant and Susceptible Tomato Lines by Elicitors of Verticillium albo-atratrum. Plant Physiol. 1990 Jan;92(1):276–280. doi: 10.1104/pp.92.1.276. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Oeller P. W., Lu M. W., Taylor L. P., Pike D. A., Theologis A. Reversible inhibition of tomato fruit senescence by antisense RNA. Science. 1991 Oct 18;254(5030):437–439. doi: 10.1126/science.1925603. [DOI] [PubMed] [Google Scholar]
  8. Penarrubia L., Aguilar M., Margossian L., Fischer R. L. An Antisense Gene Stimulates Ethylene Hormone Production during Tomato Fruit Ripening. Plant Cell. 1992 Jun;4(6):681–687. doi: 10.1105/tpc.4.6.681. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Robb J., Lee S. W., Mohan R., Kolattukudy P. E. Chemical characterization of stress-induced vascular coating in tomato. Plant Physiol. 1991 Oct;97(2):528–536. doi: 10.1104/pp.97.2.528. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Roberts E., Kolattukudy P. E. Molecular cloning, nucleotide sequence, and abscisic acid induction of a suberization-associated highly anionic peroxidase. Mol Gen Genet. 1989 Jun;217(2-3):223–232. doi: 10.1007/BF02464885. [DOI] [PubMed] [Google Scholar]
  11. Sijmons P. C., Kolattukudy P. E., Bienfait H. F. Iron Deficiency Decreases Suberization in Bean Roots through a Decrease in Suberin-Specific Peroxidase Activity. Plant Physiol. 1985 May;78(1):115–120. doi: 10.1104/pp.78.1.115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Smith C. J., Watson C. F., Morris P. C., Bird C. R., Seymour G. B., Gray J. E., Arnold C., Tucker G. A., Schuch W., Harding S. Inheritance and effect on ripening of antisense polygalacturonase genes in transgenic tomatoes. Plant Mol Biol. 1990 Mar;14(3):369–379. doi: 10.1007/BF00028773. [DOI] [PubMed] [Google Scholar]
  13. Tieman D. M., Harriman R. W., Ramamohan G., Handa A. K. An Antisense Pectin Methylesterase Gene Alters Pectin Chemistry and Soluble Solids in Tomato Fruit. Plant Cell. 1992 Jun;4(6):667–679. doi: 10.1105/tpc.4.6.667. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES