Abstract
Individual members of the Arabidopsis thaliana ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (rbcS) gene family are differentially regulated by light of different qualities. In 10-d-old etiolated seedlings, the expression of only three of the four genes is under inductive phytochrome control. rbcS mRNA levels reach a maximum (3- to 5-fold higher than the dark level) about 6 h after a red light pulse, but the rate of decay differs among the genes. Moreover, rbcS 2B requires a higher fluence for induction. At early stages of development, rbcS 1A, 2B, and 3B are highly expressed in the dark and cannot be further induced by red light, indicating a developmental component in the overall regulatory mechanism. Continuous light experiments indicate that high-irradiance responses may play a role in the induction of at least three of the four rbcS genes. Under conditions of phytochrome saturation, rbcS 1A is insensitive to blue light pulses, whereas among the three B locus genes, at least rbcS 3B appears to respond to a blue-light photoreceptor. These results add to the data suggesting that individual members of rbcS gene families in higher plants may be subject to a variety of differing regulatory mechanisms.
Full Text
The Full Text of this article is available as a PDF (1.4 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Berry-Lowe S. L., Meagher R. B. Transcriptional regulation of a gene encoding the small subunit of ribulose-1,5-bisphosphate carboxylase in soybean tissue is linked to the phytochrome response. Mol Cell Biol. 1985 Aug;5(8):1910–1917. doi: 10.1128/mcb.5.8.1910. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brusslan J. A., Tobin E. M. Light-independent developmental regulation of cab gene expression in Arabidopsis thaliana seedlings. Proc Natl Acad Sci U S A. 1992 Aug 15;89(16):7791–7795. doi: 10.1073/pnas.89.16.7791. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Clugston C. K., Barnett L. K., Urwin N. A., Jenkins G. I. Photoreceptors controlling transcription of rbcS genes in green leaf tissue of Pisum sativum. Photochem Photobiol. 1990 Jul;52(1):23–28. doi: 10.1111/j.1751-1097.1990.tb01750.x. [DOI] [PubMed] [Google Scholar]
- Coen D. M., Bedbrook J. R., Bogorad L., Rich A. Maize chloroplast DNA fragment encoding the large subunit of ribulosebisphosphate carboxylase. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5487–5491. doi: 10.1073/pnas.74.12.5487. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fluhr R., Chua N. H. Developmental regulation of two genes encoding ribulose-bisphosphate carboxylase small subunit in pea and transgenic petunia plants: Phytochrome response and blue-light induction. Proc Natl Acad Sci U S A. 1986 Apr;83(8):2358–2362. doi: 10.1073/pnas.83.8.2358. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kaufman L. S., Thompson W. F., Briggs W. R. Different Red Light Requirements for Phytochrome-Induced Accumulation of cab RNA and rbcS RNA. Science. 1984 Dec 21;226(4681):1447–1449. doi: 10.1126/science.226.4681.1447. [DOI] [PubMed] [Google Scholar]
- Manzara T., Carrasco P., Gruissem W. Developmental and organ-specific changes in promoter DNA-protein interactions in the tomato rbcS gene family. Plant Cell. 1991 Dec;3(12):1305–1316. doi: 10.1105/tpc.3.12.1305. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schindler U., Terzaghi W., Beckmann H., Kadesch T., Cashmore A. R. DNA binding site preferences and transcriptional activation properties of the Arabidopsis transcription factor GBF1. EMBO J. 1992 Apr;11(4):1275–1289. doi: 10.1002/j.1460-2075.1992.tb05171.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Simpson J., VAN Montagu M., Herrera-Estrella L. Photosynthesis-associated gene families: differences in response to tissue-specific and environmental factors. Science. 1986 Jul 4;233(4759):34–38. doi: 10.1126/science.233.4759.34. [DOI] [PubMed] [Google Scholar]
- Wanner L. A., Gruissem W. Expression dynamics of the tomato rbcS gene family during development. Plant Cell. 1991 Dec;3(12):1289–1303. doi: 10.1105/tpc.3.12.1289. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wehmeyer B., Cashmore A. R., Schäfer E. Photocontrol of the Expression of Genes Encoding Chlorophyll a/b Binding Proteins and Small Subunit of Ribulose-1,5-Bisphosphate Carboxylase in Etiolated Seedlings of Lycopersicon esculentum (L.) and Nicotiana tabacum (L.). Plant Physiol. 1990 Jul;93(3):990–997. doi: 10.1104/pp.93.3.990. [DOI] [PMC free article] [PubMed] [Google Scholar]