Abstract
Triphenyl tetrazolium chloride (TTC) reduction by cucumber (Cucumis sativus L. cv Poinsett 76 and cv Ashley) leaf discs was used as a viability assay to examine the effect of temperature pretreatment on the tissue response to acute hyperthermia. Semi-logarithmic plots of TTC reduction as a function of incubation time at different temperatures from 40 to 60[deg]C resembled the heat survival curves of animal cells. Heat inactivation rates were obtained and subjected to "quasi" Arrhenius analyses by analytical methods derived from the animal studies. The Arrhenius plots of TTC reduction rates for cv Ashley leaf discs preincubated at 25 or 37[deg]C and for cv Poinsett 76 preincubated at 37[deg]C were linear with the same activation energy (Ea) of about 80 kcal mol-1. The Arrhenius plot of cv Poinsett 76 preincubated at 25[deg]C was nonlinear with an Ea of about 80 kcal mol-1 at temperatures below 46[deg]C and an Ea of about 27.5 kcal mol-1 at temperatures above 47[deg]C. The significance of these differences is discussed in terms of the role of protein denaturation in the thermal sensitivity of cucumber disc reduction of TTC and the applicability of these methods to the analysis of plant cellular heat sensitivity.
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Selected References
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- Bauer K. D., Henle K. J. Arrhenius analysis of heat survival curves from normal and thermotolerant CHO cells. Radiat Res. 1979 May;78(2):251–263. [PubMed] [Google Scholar]
- Ferguson R. K., Vlasses P. H., Koplin J. R., Holmes G. I., Huber P., Demetriades J., Abrams W. B. Relationships among timolol doses, plasma concentrations and beta-adrenoceptor blocking activity. Br J Clin Pharmacol. 1982 Nov;14(5):719–725. doi: 10.1111/j.1365-2125.1982.tb04963.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Koval T. M. Gamma-ray- and UV-sensitive strains of a radioresistant cell line: isolation and cross-sensitivity to other agents. Radiat Res. 1991 Jul;127(1):58–63. [PubMed] [Google Scholar]
- Lafuente M. T., Belver A., Guye M. G., Saltveit M. E. Effect of temperature conditioning on chilling injury of cucumber cotyledons: possible role of abscisic Acid and heat shock proteins. Plant Physiol. 1991 Feb;95(2):443–449. doi: 10.1104/pp.95.2.443. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mahan J. R., Burke J. J., Orzech K. A. Thermal Dependence of the Apparent K(m) of Glutathione Reductases from Three Plant Species. Plant Physiol. 1990 Jun;93(2):822–824. doi: 10.1104/pp.93.2.822. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rosenberg B., Kemeny G., Switzer R. C., Hamilton T. C. Quantitative evidence for protein denaturation as the cause of thermal death. Nature. 1971 Aug 13;232(5311):471–473. doi: 10.1038/232471a0. [DOI] [PubMed] [Google Scholar]
- Westra A., Dewey W. C. Variation in sensitivity to heat shock during the cell-cycle of Chinese hamster cells in vitro. Int J Radiat Biol Relat Stud Phys Chem Med. 1971;19(5):467–477. doi: 10.1080/09553007114550601. [DOI] [PubMed] [Google Scholar]