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. 1993 Mar;101(3):1089–1096. doi: 10.1104/pp.101.3.1089

Molecular cloning of abscisic acid-responsive mRNAs expressed during the induction of freezing tolerance in bromegrass (Bromus inermis Leyss) suspension culture.

S P Lee 1, T H Chen 1
PMCID: PMC158729  PMID: 8310047

Abstract

Abscisic acid (ABA) increases the freezing tolerance of bromegrass (Bromus inermis Leyss) cell-suspension cultures at 23 degrees C and elicits many metabolic changes similar to those observed during cold acclimation. Induction and maintenance of freezing tolerance by ABA is accompanied by the expression of novel polypeptides and translatable RNAs. The objective of this study was to isolate and characterize ABA-responsive cDNAs associated with ABA-induced freezing tolerance in bromegrass cell cultures. Among the 16 ABA-responsive cDNA clones isolated, 9 were expressed only with ABA treatment, 7 showed increased transcript level, and 1 was transiently expressed. Cold responsiveness was determined in three clones with increased transcript levels and in the transiently expressed clone. Deacclimation of ABA-hardened cells was a relatively slow process, because all of the novel transcripts persisted for at least 7 d after cells were cultured in ABA-free medium. Preliminary sequencing of cDNAs has identified several clones that share high sequence homology with genes associated with sugar metabolism, osmotic stress, and protease activity. Clone pBGA61 was fully sequenced and tentatively identified as an NADPH-dependent aldose reductase. The predicted amino acid sequence of the coding region shared 92% similarity with that predicted for barley aldose reductase cDNA. It is proposed that expression of genes related to sugar metabolism and osmotic stress may be required for ABA-induced hardening.

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Selected References

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  1. Adams M. D., Kelley J. M., Gocayne J. D., Dubnick M., Polymeropoulos M. H., Xiao H., Merril C. R., Wu A., Olde B., Moreno R. F. Complementary DNA sequencing: expressed sequence tags and human genome project. Science. 1991 Jun 21;252(5013):1651–1656. doi: 10.1126/science.2047873. [DOI] [PubMed] [Google Scholar]
  2. Cattivelli L., Bartels D. Molecular cloning and characterization of cold-regulated genes in barley. Plant Physiol. 1990 Aug;93(4):1504–1510. doi: 10.1104/pp.93.4.1504. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chen H. H., Li P. H., Brenner M. L. Involvement of abscisic Acid in potato cold acclimation. Plant Physiol. 1983 Feb;71(2):362–365. doi: 10.1104/pp.71.2.362. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chen T. H., Gusta L. V. Abscisic Acid-induced freezing resistance in cultured plant cells. Plant Physiol. 1983 Sep;73(1):71–75. doi: 10.1104/pp.73.1.71. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Close T. J., Kortt A. A., Chandler P. M. A cDNA-based comparison of dehydration-induced proteins (dehydrins) in barley and corn. Plant Mol Biol. 1989 Jul;13(1):95–108. doi: 10.1007/BF00027338. [DOI] [PubMed] [Google Scholar]
  6. Crespi M. D., Zabaleta E. J., Pontis H. G., Salerno G. L. Sucrose Synthase Expression during Cold Acclimation in Wheat. Plant Physiol. 1991 Jul;96(3):887–891. doi: 10.1104/pp.96.3.887. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hajela R. K., Horvath D. P., Gilmour S. J., Thomashow M. F. Molecular Cloning and Expression of cor (Cold-Regulated) Genes in Arabidopsis thaliana. Plant Physiol. 1990 Jul;93(3):1246–1252. doi: 10.1104/pp.93.3.1246. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Houde M., Danyluk J., Laliberté J. F., Rassart E., Dhindsa R. S., Sarhan F. Cloning, characterization, and expression of a cDNA encoding a 50-kilodalton protein specifically induced by cold acclimation in wheat. Plant Physiol. 1992 Aug;99(4):1381–1387. doi: 10.1104/pp.99.4.1381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Keith C. N., McKersie B. D. The Effect of Abscisic Acid on the Freezing Tolerance of Callus Cultures of Lotus corniculatus L. Plant Physiol. 1986 Mar;80(3):766–770. doi: 10.1104/pp.80.3.766. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kurkela S., Franck M. Cloning and characterization of a cold- and ABA-inducible Arabidopsis gene. Plant Mol Biol. 1990 Jul;15(1):137–144. doi: 10.1007/BF00017731. [DOI] [PubMed] [Google Scholar]
  11. Lane B. G., Bernier F., Dratewka-Kos E., Shafai R., Kennedy T. D., Pyne C., Munro J. R., Vaughan T., Walters D., Altomare F. Homologies between members of the germin gene family in hexaploid wheat and similarities between these wheat germins and certain Physarum spherulins. J Biol Chem. 1991 Jun 5;266(16):10461–10469. [PubMed] [Google Scholar]
  12. Mohapatra S. S., Poole R. J., Dhindsa R. S. Abscisic Acid-regulated gene expression in relation to freezing tolerance in alfalfa. Plant Physiol. 1988 Jun;87(2):468–473. doi: 10.1104/pp.87.2.468. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mohapatra S. S., Poole R. J., Dhindsa R. S. Changes in Protein Patterns and Translatable Messenger RNA Populations during Cold Acclimation of Alfalfa. Plant Physiol. 1987 Aug;84(4):1172–1176. doi: 10.1104/pp.84.4.1172. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mohapatra S. S., Wolfraim L., Poole R. J., Dhindsa R. S. Molecular cloning and relationship to freezing tolerance of cold-acclimation-specific genes of alfalfa. Plant Physiol. 1989 Jan;89(1):375–380. doi: 10.1104/pp.89.1.375. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Nishimura C., Matsuura Y., Kokai Y., Akera T., Carper D., Morjana N., Lyons C., Flynn T. G. Cloning and expression of human aldose reductase. J Biol Chem. 1990 Jun 15;265(17):9788–9792. [PubMed] [Google Scholar]
  16. Nordin K., Heino P., Palva E. T. Separate signal pathways regulate the expression of a low-temperature-induced gene in Arabidopsis thaliana (L.) Heynh. Plant Mol Biol. 1991 Jun;16(6):1061–1071. doi: 10.1007/BF00016077. [DOI] [PubMed] [Google Scholar]
  17. Ray W. J., Jr, Hermodson M. A., Puvathingal J. M., Mahoney W. C. The complete amino acid sequence of rabbit muscle phosphoglucomutase. J Biol Chem. 1983 Aug 10;258(15):9166–9174. [PubMed] [Google Scholar]
  18. Robertson A. J., Gusta L. V., Reaney M. J., Ishikawa M. Protein Synthesis in Bromegrass (Bromus inermis Leyss) Cultured Cells during the Induction of Frost Tolerance by Abscisic Acid or Low Temperature. Plant Physiol. 1987 Aug;84(4):1331–1336. doi: 10.1104/pp.84.4.1331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Schaffer M. A., Fischer R. L. Analysis of mRNAs that Accumulate in Response to Low Temperature Identifies a Thiol Protease Gene in Tomato. Plant Physiol. 1988 Jun;87(2):431–436. doi: 10.1104/pp.87.2.431. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Skriver K., Mundy J. Gene expression in response to abscisic acid and osmotic stress. Plant Cell. 1990 Jun;2(6):503–512. doi: 10.1105/tpc.2.6.503. [DOI] [PMC free article] [PubMed] [Google Scholar]

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